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学科主题: 临床医学
题名:
Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene
作者: Yin, XX; Chen, ZQ; Guo, ZQ; Dang, GT; Ma, QJ; Wang, SW
关键词: telomerase ; osteoblast ; immortalization
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2004-03-12
DOI: 10.1016/j.bbrc.2003.01.102
卷: 315, 期:3, 页:643-651
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: MESENCHYMAL STEM-CELLS ; HUMAN-PAPILLOMAVIRUS TYPE-16 ; HUMAN EPITHELIAL-CELLS ; MARROW STROMAL CELLS ; CATALYTIC SUBUNIT ; BONE-MARROW ; LIFE-SPAN ; IN-VITRO ; MOUSE CALVARIAE ; T-ANTIGEN
英文摘要:

In the current study, in order to establish an immortalized osteoblast cell line, human mesenchymal stem cells (hMSCs) had been inducted into osteoblasts directionally by an osteo-inductive conditioned medium, then the osteoblasts were steadily transduced by a retroviral vector containing human telomerase reverse transcriptase (hTERT) gene. The expression of hTERT, the telomerase activity, the telomere lengths, the tumorigenesis and the osteogenesis characteristics of transduced cells at different population doublings (PDs) and the primary normal human osteoblast (hOB) were identified. The results demonstrated that hTERT gene had been transferred into human osteoblasts successfully; the transduced cell line-clone5 expressed telomerase activity and divided vigorously and now have undergone more than 120 PDs; The telomere length of clone5 elongated and was stable; Different eras of clone5 (PD 40 and PD 88) both expressed bone-specific markers, such as alkaline phosphatase (ALP), collagen type 1, and osteopontin. And the quantitative assay of ALP activity showed that there were no significant differences among untransduced cells, PD 40 and PD 88 clone5 cells. Furthermore, the immortalized cell line was benign in nude mice tumor formation and soft agar colony formation assay. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000189291900018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53598
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Cent Lab, Beijing 100044, Peoples R China

Recommended Citation:
Yin, XX,Chen, ZQ,Guo, ZQ,et al. Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,315(3):643-651.
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