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学科主题临床医学
Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene
Yin, XX; Chen, ZQ; Guo, ZQ; Dang, GT; Ma, QJ; Wang, SW
关键词Telomerase Osteoblast Immortalization
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2004-03-12
DOI10.1016/j.bbrc.2003.01.102
315期:3页:643-651
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]MESENCHYMAL STEM-CELLS ; HUMAN-PAPILLOMAVIRUS TYPE-16 ; HUMAN EPITHELIAL-CELLS ; MARROW STROMAL CELLS ; CATALYTIC SUBUNIT ; BONE-MARROW ; LIFE-SPAN ; IN-VITRO ; MOUSE CALVARIAE ; T-ANTIGEN
英文摘要

In the current study, in order to establish an immortalized osteoblast cell line, human mesenchymal stem cells (hMSCs) had been inducted into osteoblasts directionally by an osteo-inductive conditioned medium, then the osteoblasts were steadily transduced by a retroviral vector containing human telomerase reverse transcriptase (hTERT) gene. The expression of hTERT, the telomerase activity, the telomere lengths, the tumorigenesis and the osteogenesis characteristics of transduced cells at different population doublings (PDs) and the primary normal human osteoblast (hOB) were identified. The results demonstrated that hTERT gene had been transferred into human osteoblasts successfully; the transduced cell line-clone5 expressed telomerase activity and divided vigorously and now have undergone more than 120 PDs; The telomere length of clone5 elongated and was stable; Different eras of clone5 (PD 40 and PD 88) both expressed bone-specific markers, such as alkaline phosphatase (ALP), collagen type 1, and osteopontin. And the quantitative assay of ALP activity showed that there were no significant differences among untransduced cells, PD 40 and PD 88 clone5 cells. Furthermore, the immortalized cell line was benign in nude mice tumor formation and soft agar colony formation assay. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000189291900018
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53598
专题北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Cent Lab, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Yin, XX,Chen, ZQ,Guo, ZQ,et al. Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,315(3):643-651.
APA Yin, XX,Chen, ZQ,Guo, ZQ,Dang, GT,Ma, QJ,&Wang, SW.(2004).Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,315(3),643-651.
MLA Yin, XX,et al."Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 315.3(2004):643-651.
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