IR@PKUHSC  > 北京大学第一临床医学院
学科主题临床医学
Rapamycin promoted thrombosis and platelet adhesion to endothelial cells by inducing membrane remodeling
Jiang, Ping1,2; Lan, Yong2,3; Luo, Jun1,2; Ren, Ya-Li4; Liu, Dong-Ge2,5; Pang, Jian-Xin2,5; Liu, Jin6; Li, Jian1,2; Wang, Chen2,7; Cai, Jian-Ping1,2
关键词Thrombosis Membrane Remodeling Endothelial Cell Platelet
刊名BMC CELL BIOLOGY
2014-02-24
DOI10.1186/1471-2121-15-7
15
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]ELUTING STENTS ; AUTOPHAGY ; MECHANISM ; MODEL
英文摘要

Background: Recently, evidence indicated that the rapamycin-eluting stent which was used worldwide may contribute to an increased risk for thrombosis. On the contrary, other researchers found it was safe. Thus, it is necessary to clarify the effect of rapamycin on thrombosis and the corresponding mechanisms.

Results: The effects of rapamycin in vivo were evaluated by modified deep vein thrombosis animal model. The platelets were from healthy volunteers and the platelet-endothelium (purchased from ATCC) adhesion in cultured endothelial cells was assessed. Membrane rufflings in endothelial cells were examined by confocal and electron microscope. Thrombus formation increased in rats that were injected with rapamycin. Electron microscope analysis exhibited microvilli on the rapamycin-treated endothelium in rats. Rapamycin enhanced membrane ruffling in human umbilical vein endothelial cells (HUVECs) and adhesion of platelets to HUVECs. The platelet-HUVECs adhesion was attenuated when cells were treated with cytochalacin B. Inhibition of autophagy by 3-methyladenine led to suppression of membrane ruffles in HUVECs and augmentation of platelet-endothelial adhesion.

Conclusions: In conclusion, we found that endothelial membrane remodeling induced by rapamycin is crucial for the adhesion of platelets to endothelial cells and thereby for thrombosis in vivo, and that the endothelial membrane remodeling is autophagy dependent.

语种英语
WOS记录号WOS:000333055200001
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53619
专题北京大学第一临床医学院
北京大学第二临床医学院_放射科
作者单位1.Beijing Hosp, Dept Resp Med, Beijing 100730, Peoples R China
2.Beijing Hosp, Key Lab Geriatr, Beijing 100730, Peoples R China
3.Beijing Inst Geriatr, Minist Hlth, Beijing 100730, Peoples R China
4.Beijing Hosp, Dept Vasc Surg, Beijing 100730, Peoples R China
5.Beijing Hosp, Dept Pathol, Beijing 100730, Peoples R China
6.Peking Univ, Lab Electron Microscopy, Hosp 1, Beijing 100034, Peoples R China
7.Beijing Normal Univ, Coll Lifesci, Beijing 100875, Peoples R China
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GB/T 7714
Jiang, Ping,Lan, Yong,Luo, Jun,et al. Rapamycin promoted thrombosis and platelet adhesion to endothelial cells by inducing membrane remodeling[J]. BMC CELL BIOLOGY,2014,15.
APA Jiang, Ping.,Lan, Yong.,Luo, Jun.,Ren, Ya-Li.,Liu, Dong-Ge.,...&Cai, Jian-Ping.(2014).Rapamycin promoted thrombosis and platelet adhesion to endothelial cells by inducing membrane remodeling.BMC CELL BIOLOGY,15.
MLA Jiang, Ping,et al."Rapamycin promoted thrombosis and platelet adhesion to endothelial cells by inducing membrane remodeling".BMC CELL BIOLOGY 15(2014).
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