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学科主题: 临床医学
题名:
Rapamycin promoted thrombosis and platelet adhesion to endothelial cells by inducing membrane remodeling
作者: Jiang, Ping1,2; Lan, Yong2,3; Luo, Jun1,2; Ren, Ya-Li4; Liu, Dong-Ge2,5; Pang, Jian-Xin2,5; Liu, Jin6; Li, Jian1,2; Wang, Chen2,7; Cai, Jian-Ping1,2
关键词: Thrombosis ; Membrane remodeling ; Endothelial cell ; Platelet
刊名: BMC CELL BIOLOGY
发表日期: 2014-02-24
DOI: 10.1186/1471-2121-15-7
卷: 15
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: ELUTING STENTS ; AUTOPHAGY ; MECHANISM ; MODEL
英文摘要:

Background: Recently, evidence indicated that the rapamycin-eluting stent which was used worldwide may contribute to an increased risk for thrombosis. On the contrary, other researchers found it was safe. Thus, it is necessary to clarify the effect of rapamycin on thrombosis and the corresponding mechanisms.

Results: The effects of rapamycin in vivo were evaluated by modified deep vein thrombosis animal model. The platelets were from healthy volunteers and the platelet-endothelium (purchased from ATCC) adhesion in cultured endothelial cells was assessed. Membrane rufflings in endothelial cells were examined by confocal and electron microscope. Thrombus formation increased in rats that were injected with rapamycin. Electron microscope analysis exhibited microvilli on the rapamycin-treated endothelium in rats. Rapamycin enhanced membrane ruffling in human umbilical vein endothelial cells (HUVECs) and adhesion of platelets to HUVECs. The platelet-HUVECs adhesion was attenuated when cells were treated with cytochalacin B. Inhibition of autophagy by 3-methyladenine led to suppression of membrane ruffles in HUVECs and augmentation of platelet-endothelial adhesion.

Conclusions: In conclusion, we found that endothelial membrane remodeling induced by rapamycin is crucial for the adhesion of platelets to endothelial cells and thereby for thrombosis in vivo, and that the endothelial membrane remodeling is autophagy dependent.

语种: 英语
所属项目编号: 31371160 ; 201104043 ; jws1433
项目资助者: National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Scientific research foundation of the Education Ministry for returned Chinese scholars
WOS记录号: WOS:000333055200001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53619
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Beijing Hosp, Dept Resp Med, Beijing 100730, Peoples R China
2.Beijing Hosp, Key Lab Geriatr, Beijing 100730, Peoples R China
3.Beijing Inst Geriatr, Minist Hlth, Beijing 100730, Peoples R China
4.Beijing Hosp, Dept Vasc Surg, Beijing 100730, Peoples R China
5.Beijing Hosp, Dept Pathol, Beijing 100730, Peoples R China
6.Peking Univ, Lab Electron Microscopy, Hosp 1, Beijing 100034, Peoples R China
7.Beijing Normal Univ, Coll Lifesci, Beijing 100875, Peoples R China

Recommended Citation:
Jiang, Ping,Lan, Yong,Luo, Jun,et al. Rapamycin promoted thrombosis and platelet adhesion to endothelial cells by inducing membrane remodeling[J]. BMC CELL BIOLOGY,2014,15.
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