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HYALURONAN TETRASACCHARIDE IN THE CEREBROSPINAL FLUID IS ASSOCIATED WITH SELF-REPAIR OF RATS AFTER CHRONIC SPINAL CORD COMPRESSION
Wang, J.; Rong, W.; Hu, X.; Liu, X.; Jiang, L.; Ma, Y.; Dang, G.; Liu, Z.; Wei, F.
关键词Spinal Cord Injury Hyaluronan Tetrasaccharide Neurotrophic Factor Nf-kappa b C-iap(2) Caspase-3
刊名NEUROSCIENCE
2012-05-17
DOI10.1016/j.neuroscience.2012.03.016
210页:467-480
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者College of Chemistry at Beijing Normal University ; Natural Science Foundation of China ; College of Chemistry at Beijing Normal University ; Natural Science Foundation of China
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]CHRONIC MECHANICAL COMPRESSION ; NECROSIS-FACTOR-ALPHA ; CERVICAL MYELOPATHY ; EXTRACELLULAR-MATRIX ; MOUSE MACROPHAGES ; MODEL ; INJURY ; FRAGMENTS ; APOPTOSIS ; TUMOR
英文摘要

The objective of this study was to explore changes in hyaluronan levels in the cerebrospinal fluid (CSF) in a spinal cord compression model, to investigate whether hyaluronan tetrasaccharide was involved in this process, and to test the effects of hyaluronan tetrasaccharide on neuron and oligodendrocyte repair. We developed a chronic spinal cord compression model with various sizes of polymer sheets (1.5 x 0.7 x 0.3 mm(3); 5 x 1.5 x 0.7 mm(3)) that were implanted microsurgically underneath the C5-6 laminae. The rats were divided into three groups: a sham group, a mildly compressed (MC) group, and a widely compressed (WC) group. Locomotor functional evaluations revealed that the behavioral function of the MC and WC groups dropped to their lowest level from the fourth to fifth week and gradually recovered thereafter. The hyaluronan levels in the CSF gradually increased after spinal cord compression. Furthermore, hyaluronan tetrasaccharide was involved in the hyaluronan change. In addition, we found that nuclear factor kappa B (NF-kappa B) and cellular inhibitor-of-apoptosis protein 2 (c-IAP(2)) were co-expressed in neurons and oligodendrocytes, and caspase-3 expression gradually decreased in the compression model. The brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) expression was upregulated in astrocytes at the fourth week post-compression. Hyaluronan tetrasaccharide (HA(4)) induced NF-kappa B and c-IAP(2) to suppress the H2O2-induced apoptosis in primary neuronal cultures and increased BDNF and VEGF expression in astrocytic cultures in vitro. These findings suggest that HA(4) in the CSF may associate with behavioral recovery by increasing the levels of NF-kappa B, c-IAP(2), and neurotrophic factors after chronic spinal cord compression. (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.

语种英语
所属项目编号58441-06
资助者College of Chemistry at Beijing Normal University ; Natural Science Foundation of China ; College of Chemistry at Beijing Normal University ; Natural Science Foundation of China
WOS记录号WOS:000304730000043
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53622
专题北京大学第三临床医学院_骨科
作者单位Peking Univ, Hosp 3, Dept Orthoped, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Wang, J.,Rong, W.,Hu, X.,et al. HYALURONAN TETRASACCHARIDE IN THE CEREBROSPINAL FLUID IS ASSOCIATED WITH SELF-REPAIR OF RATS AFTER CHRONIC SPINAL CORD COMPRESSION[J]. NEUROSCIENCE,2012,210:467-480.
APA Wang, J..,Rong, W..,Hu, X..,Liu, X..,Jiang, L..,...&Wei, F..(2012).HYALURONAN TETRASACCHARIDE IN THE CEREBROSPINAL FLUID IS ASSOCIATED WITH SELF-REPAIR OF RATS AFTER CHRONIC SPINAL CORD COMPRESSION.NEUROSCIENCE,210,467-480.
MLA Wang, J.,et al."HYALURONAN TETRASACCHARIDE IN THE CEREBROSPINAL FLUID IS ASSOCIATED WITH SELF-REPAIR OF RATS AFTER CHRONIC SPINAL CORD COMPRESSION".NEUROSCIENCE 210(2012):467-480.
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