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Pattern-based diagnosis and screening of differentially expressed serum proteins for rheumatoid arthritis by proteomic fingerprinting
Long, Li1; Li, Ru1; Li, Yongzhe2,3; Hu, Chaojun2,3; Li, Zhanguo1
关键词Rheumatoid Arthritis Maldi-tof Mass Spectrometry Magnetic Beads
刊名RHEUMATOLOGY INTERNATIONAL
2011-08-01
DOI10.1007/s00296-010-1407-4
31期:8页:1069-1074
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
资助者National Key Technology R&amp ; D Program of china ; Medical Research and Development Capital Funds of China ; National Key Technology R&amp ; D Program of china ; Medical Research and Development Capital Funds of China
研究领域[WOS]Rheumatology
关键词[WOS]FLIGHT MASS-SPECTROMETRY ; CLASSIFICATION CRITERIA ; REVISED CRITERIA ; DISCOVERY ; CANCER ; BIOMARKERS ; SEPARATION
英文摘要

The objective of this study was to search for proteomic patterns distinguishing patients with rheumatoid arthritis (RA) from healthy controls, biomarker candidates specific for early RA, and proteins reflecting disease activity, by profiling of serum proteins using magnetic bead-based (MB) separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Magnetic chemical affinity beads were used to differentially capture serum proteins prior to MALDI-TOF analysis. Seventy serum samples from patients with RA and 50 from healthy controls were analyzed. The samples were randomly allocated to the training set or test set to develop a pattern by means of decision tree algorithm. ANOVA test was utilized to search for biomarkers for early RA. Pearson correlation analysis was employed to estimate the overexpressed peaks in relation to Disease Activity Score (DAS)28. The algorithm identified a pattern based on 3 peaks (m/z 2,490, 5,910.07, 6,436.73) that, in the training set, separated patients with RA from healthy controls with a sensitivity and specificity of 87.5 and 96.7%, respectively. Blind test data indicated a sensitivity of 86.7% and specificity of 90%. The peaks of m/z 1,014.92 and 1,061.38 were raised significantly in early RA group (disease duration < 12 months) compared with those in non-early RA group (disease duration a parts per thousand yen12 months) and healthy controls. A positive correlation was found between the intensity of peak 9,591.47 and DAS28 scores. Using MB separation followed by MALDI-TOF-MS enabled rapid diagnosis of RA according to fingerprint pattern, a method which might also help to assess disease activity and identify early RA.

语种英语
所属项目编号2008BA159B01
资助者National Key Technology R&amp ; D Program of china ; Medical Research and Development Capital Funds of China ; National Key Technology R&amp ; D Program of china ; Medical Research and Development Capital Funds of China
WOS记录号WOS:000293024300013
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53624
专题北京大学第二临床医学院
作者单位1.Peking Univ, Dept Rheumatol & Immunol, Peoples Hosp, Beijing 100044, Peoples R China
2.Beijing Union Med Coll Hosp, Dept Med Rheumatol, Beijing 100730, Peoples R China
3.Beijing Union Med Coll Hosp, Div Clin Immunol, Beijing 100730, Peoples R China
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GB/T 7714
Long, Li,Li, Ru,Li, Yongzhe,et al. Pattern-based diagnosis and screening of differentially expressed serum proteins for rheumatoid arthritis by proteomic fingerprinting[J]. RHEUMATOLOGY INTERNATIONAL,2011,31(8):1069-1074.
APA Long, Li,Li, Ru,Li, Yongzhe,Hu, Chaojun,&Li, Zhanguo.(2011).Pattern-based diagnosis and screening of differentially expressed serum proteins for rheumatoid arthritis by proteomic fingerprinting.RHEUMATOLOGY INTERNATIONAL,31(8),1069-1074.
MLA Long, Li,et al."Pattern-based diagnosis and screening of differentially expressed serum proteins for rheumatoid arthritis by proteomic fingerprinting".RHEUMATOLOGY INTERNATIONAL 31.8(2011):1069-1074.
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