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学科主题: 临床医学
题名:
Genomic amplification of the human telomerase RNA gene for differential diagnosis of cervical disorders
作者: Tu, Zheng1; Zhang, Aipeng2; Wu, Ruifang3; Jiang, Jing1; Li, Yali2; Na Wulan3; Li, Jingran1; Zhang, Yongmei2; Li, Yibing3; Chen, Zhong4,5,6; Wei, Lihui1
刊名: CANCER GENETICS AND CYTOGENETICS
发表日期: 2009-05-01
DOI: 10.1016/j.cancergencyto.2009.01.004
卷: 191, 期:1, 页:10-16
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Genetics & Heredity
研究领域[WOS]: Oncology ; Genetics & Heredity
关键词[WOS]: SQUAMOUS INTRAEPITHELIAL LESIONS ; HUMAN-PAPILLOMAVIRUS INFECTION ; RISK HUMAN-PAPILLOMAVIRUS ; REVERSE-TRANSCRIPTASE ; UTERINE CERVIX ; HTERT GENE ; HIGH EXPRESSION ; CELL CARCINOMA ; CANCER ; HPV
英文摘要:

To evaluate genomic amplification of the human telomerase RNA gene (TERC) as a supportive approach to cytopathology or histopathology in diagnosis of low-grade and high-grade uterine cervical lesions, 1,033 Chinese women at three medical centers had liquid-based thin-layer cytopathologic examination and TERC detection by fluorescence in situ hybridization (FISH). Human papillomavirus DNA testing, colposcopy with or without biopsy, and histopathologic examination were conducted as needed. In cytopathologic examination, genomic amplification of TERC was found in 30 of 659 (4.6%) normal or benign cellular changes; in 23 of 170 (13.5%) atypical squamous cells of undetermined significance (ASCUS); in 8 of 28 (28.6%) atypical squamous cells with high-grade squamous intraepithelial lesion possible (ASC-H); and in 26 of 103 (25.2%) low-grade (LSIL) and 64 of 73 (87.7%) high-grade (HSIL) squamous intraepithelial lesions; with pairwise significant difference (P < 0.05) in each, except ASC-H and LSIL (chi(2) = 0.127, P = 0.72). In histopathologic examination, TERC was amplified in 28 of 671 (4.2%) normal, inflammatory, or wart cases; in 17 of 233 (7.3%) cervical intraepithelial neoplasia grade 1 cases (CIN 1); in 27 of 39 (69.2%) CIN 2 cases; in 57 of 67 (85.1%) CIN 3 cases; and in 22 of 23 (95.7%) cervical cancer cases; with pairwise significant difference in each (P < 0.05). The number of cells with abnormal signals increased and the abnormal signal patterns were diversified with increasing severity of cervical dysplasia. FISH detection of TERC amplification may provide an effective, noninvasive approach in conjunction with cytopathologic or histopathologic evaluation for differential diagnosis of low- and high-grade cervical disorders. (C) 2009 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: WKJ 2007-3-001
项目资助者: Ministry of Health of the People&prime ; s Republic of China
WOS记录号: WOS:000266023700002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53627
Appears in Collections:北京大学第二临床医学院_妇科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Gynecol, Beijing 100044, Peoples R China
2.Gen Hosp Peoples Liberty Army, Dept Obstet & Gynecol, Beijing, Peoples R China
3.Peking Univ, Dept Obstet & Gynecol, Shenzhen Hosp, Beijing 100044, Peoples R China
4.Univ Utah, Sch Med, Div Med Genet, Salt Lake City, UT 84112 USA
5.Univ Utah, Sch Med, ARUP Labs, Salt Lake City, UT 84112 USA
6.China Med Technol, Beijing, Peoples R China

Recommended Citation:
Tu, Zheng,Zhang, Aipeng,Wu, Ruifang,et al. Genomic amplification of the human telomerase RNA gene for differential diagnosis of cervical disorders[J]. CANCER GENETICS AND CYTOGENETICS,2009,191(1):10-16.
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