IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Proteomic analysis of proteins with altered expression induced by phenylephrine in neonatal rat cardiomyocyte
Liu, N; Li, ZJ; Liu, ZQ; Liu, SY; Han, QD; Zhang, YY
关键词Phenylephrine Cardiomyocyte Proteome
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2006-01-10
27期:1页:79-81
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]IDENTIFICATION
英文摘要

In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat car diomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after a-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods, including two dimensional polyacrylamide gel electrophoresis, PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS. The good two-dimensional pattern with a high resolution and reproducibility was obtained. Totally 14 differential protein spots were found. Put of these, 11 proteins were preliminarily identified. Desmin, Txndc5, etc. were found to be up-regulated in PE-treated cardiomyocytes, while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase, etc. were found to be down-regulated. It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.

语种中文
WOS记录号WOS:000234815500021
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53629
Collection北京大学第三临床医学院_心血管内科
作者单位1.Chinese Acad Sci, Changchun Inst Appl Chem, Lab New Drug Res & Mass Spectrometry, Changchun Ctr Mass Spectrometry, Changchun 130022, Peoples R China
2.Peking Univ, Inst Vasc Med, Hosp 3, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Liu, N,Li, ZJ,Liu, ZQ,et al. Proteomic analysis of proteins with altered expression induced by phenylephrine in neonatal rat cardiomyocyte[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2006,27(1):79-81.
APA Liu, N,Li, ZJ,Liu, ZQ,Liu, SY,Han, QD,&Zhang, YY.(2006).Proteomic analysis of proteins with altered expression induced by phenylephrine in neonatal rat cardiomyocyte.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,27(1),79-81.
MLA Liu, N,et al."Proteomic analysis of proteins with altered expression induced by phenylephrine in neonatal rat cardiomyocyte".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 27.1(2006):79-81.
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