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学科主题: 基础医学
题名:
14-3-3 gamma is upregulated by in vitro ischemia and binds to protein kinase Raf in primary cultures of astrocytes
作者: Chen, XQ; Chen, JQ; Zhang, Y; Hsiao, WWL; Yu, ACH
关键词: RNA arbitrarily primed PCR ; differential gene expression ; injury ; LY294002 ; U0126
刊名: GLIA
发表日期: 2003-06-01
DOI: 10.1002/glia.10185
卷: 42, 期:4, 页:315-324
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: NECROSIS-FACTOR-ALPHA ; GENE-EXPRESSION ; CELL-DIVISION ; APOPTOSIS ; 14-3-3-PROTEINS ; INJURY ; NEURONS ; IDENTIFICATION ; INTERLEUKIN-6 ; ASSOCIATION
英文摘要:

The 14-3-3 protein family comprises critical regulatory molecules involved in signaling during cell division, proliferation, and apoptosis. Despite extensive study, the functions of the 14-3-3 proteins in brain remain unclear. 14-3-3gamma, a subtype of the 14-3-3 family of proteins, was thought to be brain- and neuron-specific. Using RNA arbitrarily primed PCR, we identified an upregulated cDNA fragment of the 14-3-3gamma gene in primary cultures of astrocytes. Using Northern blot. analysis, we confirmed this fragment was brain-specific. In cultures of astrocytes, 14-3-3gamma genes and proteins were differentially expressed at different ages and the proteins were distributed only in the cytoplasm. These results indicated that 14-3-3gamma was not neuron-specific but also expressed in astrocytes. The function of this protein in brain is unclear. Northern and Western blot analyses demonstrated that 14-3-3gamma mRNA and protein were upregulated in cultured astrocytes in an anaerobic chamber-induced ischemia model. The induction of 14-3-3gamma proteins was neither suppressed by an MAP kinase inhibitor (U0126) nor a PI-3 kinase inhibitor (LY294002). These data indicated that induction of 14-3-3gamma might not involve PI-3 and MAP kinase-dependent pathways. Using coimmunoprecipitation, we demonstrated that endogenous 14-3-3gamma bound to c-Raf-1 and p-Raf 259. As Raf is one of the critical serine/threonine kinases controlling cell growth, differentiation, and death, the binding of 14-3-3gamma to Raf indicates the critical role of this protein in ischemia-induced apoptosis and the changes in signal transduction in astrocytes in culture. (C) 2003 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000183272100001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53644
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100871, Peoples R China
4.Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China
5.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China

Recommended Citation:
Chen, XQ,Chen, JQ,Zhang, Y,et al. 14-3-3 gamma is upregulated by in vitro ischemia and binds to protein kinase Raf in primary cultures of astrocytes[J]. GLIA,2003,42(4):315-324.
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