IR@PKUHSC  > 北京大学药学院
学科主题药学
Oridonin nanosuspension was more effective than free oridonin on G(2)/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC-1 cell line
Qi, Xiaoli1; Zhang, Dianrui2; Xu, Xia1; Feng, Feifei2; Ren, Guijie1; Chu, Qianqian1; Zhang, Qiang3; Tian, Keli1
关键词Cyclin B1 Cdc2 Caspase-3 Bcl-2 Bax
刊名INTERNATIONAL JOURNAL OF NANOMEDICINE
2012
DOI10.2147/IJN.S29483
7页:1793-1804
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
研究领域[WOS]Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]PARTICLE-SIZE REDUCTION ; DRUG-DELIVERY ; IN-VIVO ; INHIBITION ; TUMORIGENESIS ; RUBESCENS ; CASPASES ; GROWTH
英文摘要

Oridonin, a diterpenoid isolated from Rabdosia rubescencs, has been reported to have antitumor effects. However, low solubility has limited its clinical applications. Preparation of drugs in the form of nanosuspensions is an extensively utilized protocol. In this study, we investigated the anticancer activity of oridonin and oridonin nanosuspension on human pancreatic carcinoma PANC-1 cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to investigate the effect of oridonin on cell growth. Propidium iodide and Hoechst 33342 staining were used to detect morphologic changes. The percentage of apoptosis and cell cycle progression was determined by flow cytometric method staining with propidium iodide. Annexin V-fluorescein isothiocyanate (FITC)/PI staining was used to evaluate cell apoptosis by flow cytometry. Caspase-3 activity was measured by spectrophotometry. The apoptotic and cell cycle protein expression were determined by Western blot analysis. Both oridonin and oridonin nanosuspension induced apoptosis and G(2)/M phase cell cycle arrest, and the latter had a more significant cytotoxic effect. The ratio of Bcl-2/Bax protein expression was decreased and caspase-3 activity was stimulated. The expression of cyclin B1 and p-cdc2 (T161) was suppressed. Our results showed that oridonin nanosuspension was more effective than free oridonin on G(2)/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC-1 cell line.

语种英语
WOS记录号WOS:000302735200001
项目编号2009CB930300
资助机构National Basic Research Program of China (973 Program)
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53660
专题北京大学药学院
北京大学药学院_药剂学系
作者单位1.Shandong Univ, Sch Med, Dept Biochem & Mol Biol, Jinan 250012, Peoples R China
2.Shandong Univ, Coll Pharm, Dept Pharmaceut, Jinan 250012, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Qi, Xiaoli,Zhang, Dianrui,Xu, Xia,et al. Oridonin nanosuspension was more effective than free oridonin on G(2)/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC-1 cell line[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2012,7:1793-1804.
APA Qi, Xiaoli.,Zhang, Dianrui.,Xu, Xia.,Feng, Feifei.,Ren, Guijie.,...&Tian, Keli.(2012).Oridonin nanosuspension was more effective than free oridonin on G(2)/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC-1 cell line.INTERNATIONAL JOURNAL OF NANOMEDICINE,7,1793-1804.
MLA Qi, Xiaoli,et al."Oridonin nanosuspension was more effective than free oridonin on G(2)/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC-1 cell line".INTERNATIONAL JOURNAL OF NANOMEDICINE 7(2012):1793-1804.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qi, Xiaoli]的文章
[Zhang, Dianrui]的文章
[Xu, Xia]的文章
百度学术
百度学术中相似的文章
[Qi, Xiaoli]的文章
[Zhang, Dianrui]的文章
[Xu, Xia]的文章
必应学术
必应学术中相似的文章
[Qi, Xiaoli]的文章
[Zhang, Dianrui]的文章
[Xu, Xia]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。