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学科主题: 药学
题名:
Oridonin nanosuspension was more effective than free oridonin on G(2)/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC-1 cell line
作者: Qi, Xiaoli1; Zhang, Dianrui2; Xu, Xia1; Feng, Feifei2; Ren, Guijie1; Chu, Qianqian1; Zhang, Qiang3; Tian, Keli1
关键词: cyclin B1 ; cdc2 ; caspase-3 ; Bcl-2 ; Bax
刊名: INTERNATIONAL JOURNAL OF NANOMEDICINE
发表日期: 2012
DOI: 10.2147/IJN.S29483
卷: 7, 页:1793-1804
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
研究领域[WOS]: Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]: PARTICLE-SIZE REDUCTION ; DRUG-DELIVERY ; IN-VIVO ; INHIBITION ; TUMORIGENESIS ; RUBESCENS ; CASPASES ; GROWTH
英文摘要:

Oridonin, a diterpenoid isolated from Rabdosia rubescencs, has been reported to have antitumor effects. However, low solubility has limited its clinical applications. Preparation of drugs in the form of nanosuspensions is an extensively utilized protocol. In this study, we investigated the anticancer activity of oridonin and oridonin nanosuspension on human pancreatic carcinoma PANC-1 cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to investigate the effect of oridonin on cell growth. Propidium iodide and Hoechst 33342 staining were used to detect morphologic changes. The percentage of apoptosis and cell cycle progression was determined by flow cytometric method staining with propidium iodide. Annexin V-fluorescein isothiocyanate (FITC)/PI staining was used to evaluate cell apoptosis by flow cytometry. Caspase-3 activity was measured by spectrophotometry. The apoptotic and cell cycle protein expression were determined by Western blot analysis. Both oridonin and oridonin nanosuspension induced apoptosis and G(2)/M phase cell cycle arrest, and the latter had a more significant cytotoxic effect. The ratio of Bcl-2/Bax protein expression was decreased and caspase-3 activity was stimulated. The expression of cyclin B1 and p-cdc2 (T161) was suppressed. Our results showed that oridonin nanosuspension was more effective than free oridonin on G(2)/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC-1 cell line.

语种: 英语
所属项目编号: 2009CB930300
项目资助者: National Basic Research Program of China (973 Program)
WOS记录号: WOS:000302735200001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53660
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Shandong Univ, Sch Med, Dept Biochem & Mol Biol, Jinan 250012, Peoples R China
2.Shandong Univ, Coll Pharm, Dept Pharmaceut, Jinan 250012, Peoples R China
3.Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100871, Peoples R China

Recommended Citation:
Qi, Xiaoli,Zhang, Dianrui,Xu, Xia,et al. Oridonin nanosuspension was more effective than free oridonin on G(2)/M cell cycle arrest and apoptosis in the human pancreatic cancer PANC-1 cell line[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2012,7:1793-1804.
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