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学科主题: 临床医学
题名:
Cytotoxic effects of mono-(2-ethylhexyl) phthalate on human embryonic stem cells
作者: Shi Cheng1; Chen Xi1; Cai Xiao-hui3; Yu Wei-dong2; Liang Rong1; Lu Qun1; Shen Huan1
关键词: human embryonic stem cell lines ; mono-(2-ethylhexyl) phthalate ; cytotoxicity ; development ; cell differentiation
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2013-05-05
DOI: 10.3760/cma.j.issn.0366-6999.20121707
卷: 126, 期:9, 页:1714-1719
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: N-BUTYL PHTHALATE ; DI(2-ETHYLHEXYL) PHTHALATE ; EXPOSURE ; DIFFERENTIATION ; DEHP ; RAT ; TESTOSTERONE ; METABOLITES ; PREGNANCY ; TOXICITY
英文摘要:

Background Mono-(2-ethylhexyl) phthalate (MEHP), the metabolite of di-(2-ethylhexyl) phthalate (DEHP), was suspected to be toxic to human embryos. This study contributes to investigating its toxic effects by an embryonic stem cell test (EST) based on two human embryonic stem cell (hESCs) lines.

Methods CH1 established in our own lab and H1, a federally registered cell line were two human embryonic stem cell lines used in this test. Four endpoint measurements were performed consisting of cell viability, proliferation ability, apoptosis as well as changes of gene expression patterns after spontaneous differentiation were determined. For measuring effects on the first three endpoints, the cells were treated with various concentrations of MEHP dissolved in dimethyl sulfoxide (DMSO) and only with DMSO which served as control and harvested after 5 days. For measuring effects during spontaneous differentiation, the RNA of embryoid bodies (EBs) formed after 8 days′ MEHP exposure was collected and changes in differentiation specific gene expression patterns were analyzed by quantitative real time RT-PCR.

Results As a result the viability and proliferation ability of both cell lines decreased significantly at 1000 mu mol/L MEHP, while there was no effect on apoptosis or cell morphology. In addition MEHP also changed the gene expression pattern in the EBs of both cell lines.

Conclusion MEHP in a high dose was cytotoxic and affected the development of hESCs, which indicates its embryo toxicity in human embryos.

语种: 英语
所属项目编号: 7112141
项目资助者: Beijing Natural Science Foundation
WOS记录号: WOS:000319529100021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53667
Appears in Collections:北京大学第二临床医学院_计划生育与生殖医学科_期刊论文

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作者单位: 1.Peking Univ, Reprod Med Ctr, Peoples Hosp, Beijing 100044, Peoples R China
2.Peking Univ, Inst Clin Mol Biol, Peoples Hosp, Beijing 100044, Peoples R China
3.Capital Med Univ, Beijing Friendship Hosp, Dept Obstet & Gynecol, Beijing 100050, Peoples R China

Recommended Citation:
Shi Cheng,Chen Xi,Cai Xiao-hui,et al. Cytotoxic effects of mono-(2-ethylhexyl) phthalate on human embryonic stem cells[J]. CHINESE MEDICAL JOURNAL,2013,126(9):1714-1719.
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