北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 药理学系  > 期刊论文
学科主题: 基础医学
题名:
Establishment of a T-Ag and NF-kappa B binding elements gene co-transfected stable HUVECs cell line
作者: Liu, YX; Cao, WS; Irani, K; Li, XJ
关键词: cell line ; co-transfection ; T-Ag ; NF-kappa B ; reporter gene activity assay
刊名: JOURNAL OF CELLULAR BIOCHEMISTRY
发表日期: 2003-08-15
DOI: 10.1002/jcb.10588
卷: 89, 期:6, 页:1285-1290
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
英文摘要:

To set up a T-Ag gene transfected stable human umbilical venous endothelial cells (HUVECs) cell line and a T-Ag, nuclear transcriptional factor kappa B (NF-kappaB) binding elements linked with luciferase reporter gene co-transfected stable HUVECs cell line. Cultured HUVECs were transfected with pCl-neo-T-Ag and pRSV-luc-3XkappaB by lipofectin. The G418 selected monoclones were subcultured. The expression of marker protein, vWF and the characteristic of uptake of lipids were compared by Western blotting and immunocytochemistry in non-transfected and transfected HUVECs. The reporter gene assay was done in the presence of TNF-alpha. A T-Ag gene transfected stable HUVECs cell line and a T-Ag and NF-kappaB binding elements linked with luciferase reporter gene co-transfected stable HUVECs cell lines were set up. The expression of vWF of these cell lines was similar with those in non-transformed HUVECs. The function of uptaking of lipids was preserved as well in transfected cell lines. Furthermore, TNF-alpha, a typical cytokine increasing the activity of NF-kappaB was used to treat the transfected cells O/N. The higher luciferase reporter gene activity was seen. A pCl-neo-T-Ag and pRSV-luc-3X kappaB co-transfected stable HUVECs cell line might be used to check reporter gene activity directly. It might be a useful tool to screen drugs acting on transcription level. (C) 2003 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000184609400018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53672
Appears in Collections:基础医学院_药理学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Johns Hopkins Univ Hosp, Sch Med, Div Cardiol, Baltimore, MD 21205 USA

Recommended Citation:
Liu, YX,Cao, WS,Irani, K,et al. Establishment of a T-Ag and NF-kappa B binding elements gene co-transfected stable HUVECs cell line[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2003,89(6):1285-1290.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, YX]'s Articles
[Cao, WS]'s Articles
[Irani, K]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, YX]‘s Articles
[Cao, WS]‘s Articles
[Irani, K]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace