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学科主题临床医学
Adoptive transfer of tumor-reactive transforming growth factor-beta-1 insensitive cytolytic T cells for treatment of established mouse Renca tumors
Qin, Weijun; Tian, Feng; Wang, Fuli; Song, Bin; Wang, He; Zhang, Qiang; Jovanovic, Borko; Liang, Lili; Guo, Yinglu; Smith, Norm; Lee, Chung
刊名UROLOGY
2008-10-01
DOI10.1016/j.urology.2008.04.017
72期:4页:943-947
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]TGF-BETA ; CANCER ; CARCINOMA ; EXPRESSION ; ANTIGENS
英文摘要

OBJECTIVES To test, using a mouse renal cancer, Renca, whether adoptive transfer of tumor-reactive transforming growth factor (TOF)-beta-insensitive cytolytic T cells can inhibit tumor progression.

METHODS Cytolytic T cells were isolated from the spleen of male Balb/c mice repeatedly primed with irradiated Renca cells. They were expanded ex vivo and were rendered TGF-beta-insensitive by infecting with a retrovirus containing dominant negative TGF-beta type II receptor.

RESULTS These tumor reactive TGF-beta-insensitive cytolytic T cells showed a specific and robust tumor killing activity against Renca cells, but not irrelevant cells, using an in vitro cytotoxic assay. Adoptive transfer of cytolytic T cells was performed in mice 10 days after they were challenged with Renca cells (5 X 10(5)) by tail vein injection. At 30 days after the adoptive transfer, the pulmonary tumor Counts in mice who had received TGF-beta-insensitive cytolytic T cells (mean +/- standard deviation 130 140) was significantly less than those in mice that had received TGF-beta-sensitive cytolytic T cells (305 +/- 60) or in mice had received naive cytolytic T cells (375 +/- 50; P <.01). Kaplan-Meier survival analysis indicated that mice that had received adoptive transfer of TGF-beta-insensitive cytolytic T cells had a significantly greater rate of survival (75%) compared with mice that had received TGF-beta-sensitive cytolytic T cells (35%) or naive cytolytic T cells (15%), respectively (P <.05).

CONCLUSIONS These results suggest that adoptive transfer of tumor- re active TGF-beta-insensitive cytolytic T cells can warrant consideration for renal cell cancer immunotherapy.

语种英语
WOS记录号WOS:000259845600054
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53675
专题北京大学第一临床医学院_泌尿外科
作者单位1.Fourth Mil Med Univ, Xijing Hosp, Dept Urol, Xian 710032, Shanxi Province, Peoples R China
2.Peking Univ, Hosp 1, Dept Urol, Beijing 100871, Peoples R China
3.NW Univ Feinberg, Sch Med, H Lurie Comprehens Canc Ctr, Dept Urol, Chicago, IL USA
4.NW Univ Feinberg, Sch Med, H Lurie Comprehens Canc Ctr, Dept Prevent Med, Chicago, IL USA
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GB/T 7714
Qin, Weijun,Tian, Feng,Wang, Fuli,et al. Adoptive transfer of tumor-reactive transforming growth factor-beta-1 insensitive cytolytic T cells for treatment of established mouse Renca tumors[J]. UROLOGY,2008,72(4):943-947.
APA Qin, Weijun.,Tian, Feng.,Wang, Fuli.,Song, Bin.,Wang, He.,...&Lee, Chung.(2008).Adoptive transfer of tumor-reactive transforming growth factor-beta-1 insensitive cytolytic T cells for treatment of established mouse Renca tumors.UROLOGY,72(4),943-947.
MLA Qin, Weijun,et al."Adoptive transfer of tumor-reactive transforming growth factor-beta-1 insensitive cytolytic T cells for treatment of established mouse Renca tumors".UROLOGY 72.4(2008):943-947.
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