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学科主题: 药学
题名:
Chromatographic Fingerprint Analysis of Semen Ziziphi spinosae by HPLC-DAD Method
作者: Zeng, Wenzhi; Bai, Yanjing; Zhang, Qingying1; Zhao, Yuying
关键词: Chromatographic fingerprint ; HPLC ; Semen Ziziphi spinosae ; Ziziphus ; Ziziphus jujuba var ; spinosa
刊名: ANALYTICAL LETTERS
发表日期: 2009
DOI: 10.1080/00032710802585642
卷: 42, 期:2, 页:205-215
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要:

Reliable, reproducible and valid fingerprint analysis methods using high-performance liquid chromatography-photodiode array detection (HPLC-DAD) for characteristic bioactive flavonoids and saponins of Semen Ziziphi spinosae (SZS) were developed and validated. HPLC separation of the chemical constituents of SZS was performed on an YMC-PACK ODS-A RP-18 column and detected at 270 and 204nm for flavonoids and saponins, respectively. A mobile phase consisted of acetonitrile and 0.1% phosphoric acid aqueous solution was used with linear gradient elution. Using spinosin and jujuboside B as the reference markers of flavonoids and saponins respectively, 9 common fingerprint peaks of flavonoids and 10 common fingerprint peaks of saponins were specified based on the fingerprint analysis of 10 batches of SZS from different regions in China. The fingerprint analysis methods developed are reliable, reproducible and valid, and might be used as a more convenient approach for the species identification and quality monitoring and assessment of SZS.

语种: 英语
所属项目编号: 985-2-063-112 ; 20432030 ; 20742005
项目资助者: Program for Changjiang Scholar ; Innovative Team in University ; National Natural Science Foundation of China
WOS记录号: WOS:000263553400001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53676
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing, Peoples R China

Recommended Citation:
Zeng, Wenzhi,Bai, Yanjing,Zhang, Qingying,et al. Chromatographic Fingerprint Analysis of Semen Ziziphi spinosae by HPLC-DAD Method[J]. ANALYTICAL LETTERS,2009,42(2):205-215.
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