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学科主题: 口腔医学
题名:
Adenoviral vector-mediated overexpression of osteoprotegerin accelerates osteointegration of titanium implants in ovariectomized rats
作者: Yin, G.1; Chen, J.2; Wei, S.3; Wang, H.1; Chen, Q.1; Lin, Y.1; Hu, J.1; Luo, E.1,2
刊名: GENE THERAPY
发表日期: 2015-08-01
DOI: 10.1038/gt.2015.34
卷: 22, 期:8, 页:636-644
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]: RECEPTOR ACTIVATOR ; GENE-THERAPY ; RECOMBINANT ADENOVIRUS ; OSTEOGENIC RESPONSE ; BONE-RESORPTION ; IMMUNE-RESPONSE ; RANKL ; OSTEOCLASTOGENESIS ; OSTEOPOROSIS ; OSTEOPENIA
英文摘要:

This study investigated the efficacy of human osteoprotegerin (hOPG) transgene to accelerate osteointegration of titanium implant in ovariectomized (OVX) rats. Bone marrow stromal cells transduced with Ad-hOPG-EGFP could sustainedly express hOPG. Osteoclast precursor RAW264.7 cells treated by the hOPG were examined by tartrate-resistant acid phosphatase (TRAP) staining and bone slice resorption assay. The results showed differentiation and function of osteoclasts were significantly suppressed by hOPG in vitro. Ad-hOPG-EGFP was locally administered to the bone defect prior to implant placement in OVX and sham rats. After 3, 7, 28 days of implantation, the femurs were harvested for molecular and histological analyses. Successful transgene expression was confirmed by western blot and cryosectioning. A significant reduction in TRAP+ numbers was detected in Ad-hOPG-EGFP group. Real-time reverse transcriptase-PCR examination revealed that hOPG transgene markedly diminished the expression of cathepsin K and receptor activator for nuclear factor-. B ligand in vivo. The transgene hOPG modification revealed a marked increasing osteointegration and restored implant stability in OVX rats (P < 0.01), compared with the control groups (Ad-EGFP or sterilized phosphate-buffered saline) 28 days after implantation. In conclusion, hOPG via direct adenovirus-mediated gene transfer could accelerate osteointegration of titanium implants in OVX rats. Osteoprotegerin gene therapy may be an effective strategy to osteointegration of implants under osteoporotic conditions.

语种: 英语
所属项目编号: 81321002 ; 2014SZ0019 ; 2012CB933902
项目资助者: NSFC ; Key Technologies Research and Development Program of Science and Technology Department of Sichuan Province ; National Basic Research Program of China (973 Program)
WOS记录号: WOS:000359265000005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53678
Appears in Collections:北京大学口腔医学院_口腔颌面外科_期刊论文

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作者单位: 1.Tufts Univ Sch Dent Med, Dept Gen Dent, Div Oral Biol, Boston, MA USA
2.Sichuan Univ, State Key Lab Oral Dis, West China Hosp Stomatol, Chengdu 610041, Sichuan Provinc, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Lab Interdisciplinary Studies, Dept Oral & Maxillofacial Surg, Beijing 100871, Peoples R China

Recommended Citation:
Yin, G.,Chen, J.,Wei, S.,et al. Adenoviral vector-mediated overexpression of osteoprotegerin accelerates osteointegration of titanium implants in ovariectomized rats[J]. GENE THERAPY,2015,22(8):636-644.
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