北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Over-expression of IL-33 leads to spontaneous pulmonary inflammation in mIL-33 transgenic mice
作者: Xiang, Zhiguang1; Chen, Wei1; Ravary, Steven1; Dong, Wei1; Zhang, Wei1; Mu, Rong2; Li, Zhanguo2; Zhang, Lianfeng1
关键词: IL-33 ; sST2 ; Transgenic mice ; Cleavage ; Pulmonary inflammation
刊名: IMMUNOLOGY LETTERS
发表日期: 2010-07-08
DOI: 10.1016/j.imlet.2010.04.005
卷: 131, 期:2, 页:159-165
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: HUMAN MAST-CELLS ; AIRWAY INFLAMMATION ; CYTOKINE PRODUCTION ; HUMAN EOSINOPHILS ; ST2 RECEPTOR ; IN-VIVO ; INTERLEUKIN-33 ; PROTEIN ; MATURATION ; CASPASE-1
英文摘要:

IL-33 plays an important role in inflammatory diseases including hypersensitive diseases like asthma, autoimmune diseases like rheumatoid arthritis, cardiovascular diseases like heart failure and neurodegenerative diseases like Alzheimer′s disease. Here we reported the generation of an IL-33 transgenic mouse, in which mouse IL-33 full-length cDNA was controlled under the CMV promoter. The transgenic IL-33 was released as a cleaved form with molecular weight of 18 kDa in pulmonary, nephritic, cardiac and pancreatic tissues in transgenic mice and the pI of 18 kDa peptide was about pH 3-5 on the 2D PAGE which was similar with the activated peptide of IL-33. Histological analysis showed massive airway inflammation with infiltration of eosinophils around bronchi and small blood vessels, hyperplasia of goblet cells and accumulation of mucus-like material in pulmonary tissue of transgenic mice. An increase of IL-5, IL-8, IL-13 and IgE was detected in bronchoalveolar lavage fluid (BALF) of transgenic mice, which are inflammatory factors. These findings suggest transgenic IL-33 could be cleaved and secreted in an activated form and play an important role in the pathogenesis of pulmonary inflammation. (C) 2010 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 200802036 ; 2009ZX09501-026 ; 2010CB529100
项目资助者: Ministry of Health, China ; National Science and Technology ; 973 projects
WOS记录号: WOS:000279703800008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53683
Appears in Collections:北京大学第二临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Med Sci, Peking Union Med Coll, Inst Lab Anim Sci, Key Lab Human Dis Comparat Med,Minist Hlth, Beijing 100021, Peoples R China
2.Peking Univ, Dept Rheumatol & Immunol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Xiang, Zhiguang,Chen, Wei,Ravary, Steven,et al. Over-expression of IL-33 leads to spontaneous pulmonary inflammation in mIL-33 transgenic mice[J]. IMMUNOLOGY LETTERS,2010,131(2):159-165.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xiang, Zhiguang]'s Articles
[Chen, Wei]'s Articles
[Ravary, Steven]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xiang, Zhiguang]‘s Articles
[Chen, Wei]‘s Articles
[Ravary, Steven]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace