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学科主题: 临床医学
题名:
Inhibition of human gastric carcinoma cell growth in vitro by a polysaccharide from Aster tataricus
作者: Zhang, Yunxin1; Wang, Qiusheng1; Wang, Tie2; Zhang, Haikui3; Tian, Ying4; Luo, Hong5,6; Yang, Shen1; Wang, Yuan1; Huang, Xun1
关键词: Aster tataricus ; Polysaccharide ; Purification ; Apoptosis ; Human gastric cancer
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发表日期: 2012-11-01
DOI: 10.1016/j.ijbiomac.2012.06.019
卷: 51, 期:4, 页:509-513
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: TRITERPENOID SAPONINS ; CYTO-TOXICITY ; CANCER ; QUANTITATION ; LINGULATUS ; APOPTOSIS ; SUGARS
英文摘要:

A water-soluble polysaccharide (WATP), with a molecular weight of 6.3 x 10(4) Da, was isolated from Aster tataricus. According to gas chromatography (GC) analysis, WATP was composed of galactose, glucose, fucose, rhamnose, arabinose and mannose with molar ratios of 2.1:1.3:0.9:0.5:0.3:0.6. The effects of WATP on cell proliferation and apoptosis in human gastric cancer SGC-7901 cells were examined. MTT assay showed that WATP had a perfectly tumor growth inhibitory activity on SGC-7901 cells, but no cytotoxicity on SGC-7901 and primary human polymorphonuclear (PMN) cells analyzed using LDH assay. Flow cytometry analysis indicated that WATP could significantly induce apoptosis of SGC-7901 cells. Furthermore using Rh123 and Fluo-3 as fluorescent probes, respectively, it was found that mitochondrial transmembrane potential (Delta Psi(m)) of treatment groups was significantly lower than that in un-treatment group and the concentration of calcium in cells exposed to WATP for 24 h was increased in a dose dependent manner compared with unexposed group. These results suggest that WATP induces apoptosis of SGC-7901 cells through calcium- and Delta Psi(m)-dependent pathways, indicating that it is potentially useful as a natural anti-cancer agent. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000309310500024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53704
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Xingtai Peoples Hosp, Dept Anesthesiol, Xingtai 054031, Peoples R China
2.Beijing Anorectal Hosp, Dept Surg, Beijing 100032, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Gen Surg, Beijing 100044, Peoples R China
4.Cangzhou Hosp Integrated Tradit Chinese & Western, Dept Surg, Cangzhou 061000, Peoples R China
5.Capital Med Univ, Dept Neurobiol, Beijing 100069, Peoples R China
6.Capital Med Univ, Beijing Inst Neurosci, Beijing 100069, Peoples R China

Recommended Citation:
Zhang, Yunxin,Wang, Qiusheng,Wang, Tie,et al. Inhibition of human gastric carcinoma cell growth in vitro by a polysaccharide from Aster tataricus[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2012,51(4):509-513.
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