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学科主题: 临床医学
题名:
Saturated Fatty Acids Up-regulate COX-2 Expression in Prostate Epithelial Cells via Toll-like Receptor 4/NF-kappa B Signaling
作者: Liu, Jie1,2; Hu, Shuai1,2; Cui, Yun1,2; Sun, Meng-Kui1,2; Xie, Feng1,2; Zhang, Qian1,2; Jin, Jie1,2
关键词: saturated fatty acid ; prostate ; COX-2 ; NF-kappa B ; TLR4
刊名: INFLAMMATION
发表日期: 2014-04-01
DOI: 10.1007/s10753-013-9760-6
卷: 37, 期:2, 页:467-477
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Immunology
研究领域[WOS]: Cell Biology ; Immunology
关键词[WOS]: CHRONIC INFLAMMATION ; INSULIN-RESISTANCE ; DIETARY-FAT ; MOUSE MODEL ; CANCER ; CYCLOOXYGENASE-2 ; ADENOCARCINOMA ; GROWTH ; PROLIFERATION ; HYPERPLASIA
英文摘要:

Cyclooxygenase-2 (COX-2) has been implicated in prostate carcinogenesis, and recently it has been confirmed to be a molecular target of saturated fatty acids (SFAs). In the present study, we investigated the effect of stearic acid (SA) and palmitic acid (PA), two of the most abundant SFAs contained in dietary fat, on COX-2 expression in prostate epithelial cells and the signaling transduction pathway involved. First, we demonstrated that both SA and PA increased the mRNA and protein expression of COX-2, and consistently induced the activation of NF-kappa B in RWPE-1, BPH-1 and PC-3 prostate epithelial cell lines. The effect of SA and PA on COX-2 over-expression and NF-kappa B activation was in a dose-dependent manner, and PA was more potent than SA at the same concentration. Then, we demonstrated inhibition of NF-kappa B using its specific inhibitor strikingly attenuated PA-induced COX-2 expression. Toll-like receptor 4 (TLR4) was revealed to be expressed on RWPE-1, BPH-1 and PC-3 cell lines by PCR and immunofluorescence staining, and blocking its signaling significantly inhibited PA induced COX-2 over-expression and NF-kappa B activation. Taken together, we demonstrated that SFAs can up-regulate COX-2 expression in prostate epithelial cells, and this effect was mediated mainly through the TLR4/NF-kappa B signaling pathway.

语种: 英语
所属项目编号: 30973007
项目资助者: National Nature Science Foundation of China
WOS记录号: WOS:000332715000021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53716< E lass="metadataFieldLabel">Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China

Recommended Citation:
Liu, Jie,Hu, Shuai,Cui, Yun,et al. Saturated Fatty Acids Up-regulate COX-2 Expression in Prostate Epithelial Cells via Toll-like Receptor 4/NF-kappa B Signaling[J]. INFLAMMATION,2014,37(2):467-477.
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