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Saturated Fatty Acids Up-regulate COX-2 Expression in Prostate Epithelial Cells via Toll-like Receptor 4/NF-kappa B Signaling
Liu, Jie1,2; Hu, Shuai1,2; Cui, Yun1,2; Sun, Meng-Kui1,2; Xie, Feng1,2; Zhang, Qian1,2; Jin, Jie1,2
关键词Saturated Fatty Acid Prostate Cox-2 Nf-kappa b Tlr4
刊名INFLAMMATION
2014-04-01
DOI10.1007/s10753-013-9760-6
37期:2页:467-477
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Immunology
研究领域[WOS]Cell Biology ; Immunology
关键词[WOS]CHRONIC INFLAMMATION ; INSULIN-RESISTANCE ; DIETARY-FAT ; MOUSE MODEL ; CANCER ; CYCLOOXYGENASE-2 ; ADENOCARCINOMA ; GROWTH ; PROLIFERATION ; HYPERPLASIA
英文摘要

Cyclooxygenase-2 (COX-2) has been implicated in prostate carcinogenesis, and recently it has been confirmed to be a molecular target of saturated fatty acids (SFAs). In the present study, we investigated the effect of stearic acid (SA) and palmitic acid (PA), two of the most abundant SFAs contained in dietary fat, on COX-2 expression in prostate epithelial cells and the signaling transduction pathway involved. First, we demonstrated that both SA and PA increased the mRNA and protein expression of COX-2, and consistently induced the activation of NF-kappa B in RWPE-1, BPH-1 and PC-3 prostate epithelial cell lines. The effect of SA and PA on COX-2 over-expression and NF-kappa B activation was in a dose-dependent manner, and PA was more potent than SA at the same concentration. Then, we demonstrated inhibition of NF-kappa B using its specific inhibitor strikingly attenuated PA-induced COX-2 expression. Toll-like receptor 4 (TLR4) was revealed to be expressed on RWPE-1, BPH-1 and PC-3 cell lines by PCR and immunofluorescence staining, and blocking its signaling significantly inhibited PA induced COX-2 over-expression and NF-kappa B activation. Taken together, we demonstrated that SFAs can up-regulate COX-2 expression in prostate epithelial cells, and this effect was mediated mainly through the TLR4/NF-kappa B signaling pathway.

语种英语
WOS记录号WOS:000332715000021
项目编号30973007
资助机构National Nature Science Foundation of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53716
专题北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Liu, Jie,Hu, Shuai,Cui, Yun,et al. Saturated Fatty Acids Up-regulate COX-2 Expression in Prostate Epithelial Cells via Toll-like Receptor 4/NF-kappa B Signaling[J]. INFLAMMATION,2014,37(2):467-477.
APA Liu, Jie.,Hu, Shuai.,Cui, Yun.,Sun, Meng-Kui.,Xie, Feng.,...&Jin, Jie.(2014).Saturated Fatty Acids Up-regulate COX-2 Expression in Prostate Epithelial Cells via Toll-like Receptor 4/NF-kappa B Signaling.INFLAMMATION,37(2),467-477.
MLA Liu, Jie,et al."Saturated Fatty Acids Up-regulate COX-2 Expression in Prostate Epithelial Cells via Toll-like Receptor 4/NF-kappa B Signaling".INFLAMMATION 37.2(2014):467-477.
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