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学科主题: 基础医学
题名:
POU homeodomain protein OCT1 modulates islet 1 expression during cardiac differentiation of P19CL6 cells
作者: Liu, Yinan1; Li, Yanming1; Li, Tao3; Lu, Huafei1; Jia, Zhuqing1,2; Wang, Weiping1,2; Chen, Ping1,2; Ma, Kangtao1,2; Zhou, Chunyan1,2
关键词: Isl1 ; Cardiac progenitors ; Differentiation ; P19CL6 cells ; Octamer motif ; OCT1
刊名: CELLULAR AND MOLECULAR LIFE SCIENCES
发表日期: 2011-06-01
DOI: 10.1007/s00018-010-0544-y
卷: 68, 期:11, 页:1969-1982
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: 2ND HEART FIELD ; TRANSCRIPTIONAL REPRESSION ; PROGENITOR CELLS ; CARDIOVASCULAR PROGENITORS ; PROMOTER ACTIVATION ; WNT/BETA-CATENIN ; GENE-EXPRESSION ; DOWN-REGULATION ; SMOOTH-MUSCLE ; BINDING
英文摘要:

Islet 1 (ISL1), a marker of cardiac progenitors, plays a crucial role in cardiogenesis. However, the precise mechanism underlying the activation of its expression is not fully understood. Using the cardiac differentiation model of P19CL6 cells, we show that POU homeodomain protein, OCT1, modulates Isl1 expression in the process of cardiac differentiation. Oct1 knock-down resulted in reduction of Isl1 expression and downregulated mesodermal, cardiac-specific, and signal pathway gene expression. Additionally, the octamer motif located in the proximal region of Isl1 promoter is essential to Isl1 transcriptional activation. Mutation of this motif remarkably decreased Isl1 transcription. Although both OCT1 and OCT4 bound to this motif, it was OCT1 rather than OCT4 that modulated Isl1 expression. Furthermore, the correlation of OCT1 in regulation of Isl1 was revealed by in situ hybridization in early embryos. Collectively, our data highlight a novel role of OCT1 in the regulation of Isl1 expression.

语种: 英语
所属项目编号: 30871253 ; 90919022 ; B07001
项目资助者: National Natural Sciences Foundation of China ; Ministry of Education of China
WOS记录号: WOS:000290487100013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53718
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
3.Zhejiang Normal Univ, Dept Biol, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China

Recommended Citation:
Liu, Yinan,Li, Yanming,Li, Tao,et al. POU homeodomain protein OCT1 modulates islet 1 expression during cardiac differentiation of P19CL6 cells[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2011,68(11):1969-1982.
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