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POU homeodomain protein OCT1 modulates islet 1 expression during cardiac differentiation of P19CL6 cells
Liu, Yinan1; Li, Yanming1; Li, Tao3; Lu, Huafei1; Jia, Zhuqing1,2; Wang, Weiping1,2; Chen, Ping1,2; Ma, Kangtao1,2; Zhou, Chunyan1,2
关键词Isl1 Cardiac Progenitors Differentiation P19cl6 Cells Octamer Motif Oct1
刊名CELLULAR AND MOLECULAR LIFE SCIENCES
2011-06-01
DOI10.1007/s00018-010-0544-y
68期:11页:1969-1982
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
资助者National Natural Sciences Foundation of China ; Ministry of Education of China ; National Natural Sciences Foundation of China ; Ministry of Education of China
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]2ND HEART FIELD ; TRANSCRIPTIONAL REPRESSION ; PROGENITOR CELLS ; CARDIOVASCULAR PROGENITORS ; PROMOTER ACTIVATION ; WNT/BETA-CATENIN ; GENE-EXPRESSION ; DOWN-REGULATION ; SMOOTH-MUSCLE ; BINDING
英文摘要

Islet 1 (ISL1), a marker of cardiac progenitors, plays a crucial role in cardiogenesis. However, the precise mechanism underlying the activation of its expression is not fully understood. Using the cardiac differentiation model of P19CL6 cells, we show that POU homeodomain protein, OCT1, modulates Isl1 expression in the process of cardiac differentiation. Oct1 knock-down resulted in reduction of Isl1 expression and downregulated mesodermal, cardiac-specific, and signal pathway gene expression. Additionally, the octamer motif located in the proximal region of Isl1 promoter is essential to Isl1 transcriptional activation. Mutation of this motif remarkably decreased Isl1 transcription. Although both OCT1 and OCT4 bound to this motif, it was OCT1 rather than OCT4 that modulated Isl1 expression. Furthermore, the correlation of OCT1 in regulation of Isl1 was revealed by in situ hybridization in early embryos. Collectively, our data highlight a novel role of OCT1 in the regulation of Isl1 expression.

语种英语
所属项目编号30871253 ; 90919022 ; B07001
资助者National Natural Sciences Foundation of China ; Ministry of Education of China ; National Natural Sciences Foundation of China ; Ministry of Education of China
WOS记录号WOS:000290487100013
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53718
专题基础医学院
作者单位1.Peking Univ, Dept Biochem & Mol Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
3.Zhejiang Normal Univ, Dept Biol, Coll Chem & Life Sci, Jinhua 321004, Zhejiang, Peoples R China
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GB/T 7714
Liu, Yinan,Li, Yanming,Li, Tao,et al. POU homeodomain protein OCT1 modulates islet 1 expression during cardiac differentiation of P19CL6 cells[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2011,68(11):1969-1982.
APA Liu, Yinan.,Li, Yanming.,Li, Tao.,Lu, Huafei.,Jia, Zhuqing.,...&Zhou, Chunyan.(2011).POU homeodomain protein OCT1 modulates islet 1 expression during cardiac differentiation of P19CL6 cells.CELLULAR AND MOLECULAR LIFE SCIENCES,68(11),1969-1982.
MLA Liu, Yinan,et al."POU homeodomain protein OCT1 modulates islet 1 expression during cardiac differentiation of P19CL6 cells".CELLULAR AND MOLECULAR LIFE SCIENCES 68.11(2011):1969-1982.
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