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学科主题: 临床医学
题名:
Identification of matrix metalloproteinase 11 as a predictive tumor marker in serum based on gene expression profiling
作者: Yang, Yan-Hong1; Deng, Hua1; Li, Wen-Mei1; Zhang, Qing-Yun2; Hu, Xiao-Ting2; Xiao, Bai3; Zhu, Hai-Hong4; Geng, Pai-Li4; Lu, You-Yong1
刊名: CLINICAL CANCER RESEARCH
发表日期: 2008
DOI: 10.1158/1078-0432.CCR-07-1179
卷: 14, 期:1, 页:74-81
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: GASTRIC-CANCER CELLS ; MATRIX-METALLOPROTEINASE ; BREAST-CARCINOMA ; STROMELYSIN-3 ; METASTASIS ; INVASION ; DEATH ; MMP-9
英文摘要:

Purpose: Prognostic markers discovery is a strategy for early diagnosis and individualization therapy for human cancer. In this study, we focus to integrate different methods to identify specific biomarker and elucidate its clinical significance.

Experimental Design: A powerful tool named Digital Gene Expression Display online was applied to isolate differentially expressed genes correlated with gastric cancer. Matrix metalloproteinase 11 (MMP11) was selected and confirmed at both mRNA and protein level in 10 cell lines, 123 cases of tumor tissues, and 305 cases of gastric cancer serum specimen by serniquantitative PCR, immunohistochemistry staining, and ELISA techniques, respectively.

Results: Our data showed that overexpression of MMP11 at mRNA and protein level was consistently detected in cell lines and primary tumors compared with matched normal tissues. Importantly, serum MMP11 levels were also significantly elevated in gastric cancer patients compared with those of the control subjects (P < 0.001), and the positive expression was well correlated with metastasis in gastric cancer patients (P = 0.009).. Furthermore, we have shown that overexpression of MMP11 was associated with the malignant proliferation of AGS cells.

Conclusions: Combination of gene expression profiling and specific clinical resource is a promising approach to validate gene expression patterns associated with malignant phenotype. As a secreted protein, MMP11 may play an important role in carcinogenesis and has potential implication as a biomarker for the diagnosis and prognosis of human cancers including gastric cancer.

语种: 英语
WOS记录号: WOS:000252138800013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53724
Appears in Collections:北京大学临床肿瘤学院_分子肿瘤学研究室_期刊论文

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作者单位: 1.Peking Univ, Mol Oncol Lab, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China
2.Peking Univ, Dept Clin Lab, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100871, Peoples R China
3.Capital Med Univ, Beijing Chao Yang Hosp, Basic Med Res Ctr, Beijing, Peoples R China
4.Qinghai Med Coll, Dept Immunol, Xining, Peoples R China

Recommended Citation:
Yang, Yan-Hong,Deng, Hua,Li, Wen-Mei,et al. Identification of matrix metalloproteinase 11 as a predictive tumor marker in serum based on gene expression profiling[J]. CLINICAL CANCER RESEARCH,2008,14(1):74-81.
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