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Study on DNA strand breaks induced by sodium nitroprusside, a nitric oxide donor, in vivo and in vitro
Lin, WC; Wei, XT; Xue, HW; Kelimu, M; Tao, R; Song, YS; Zhou, ZC
关键词Nitric Oxide Sodium Nitroprusside Dna Single-strand Breaks In Vitro In Vivo
刊名MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
2000-03-23
466期:2页:187-195
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
关键词[WOS]ELECTROPHORESIS COMET ASSAY ; SJL MICE ; IN-VIVO ; PEROXYNITRITE CAUSES ; MEDIATED APOPTOSIS ; REACTIVE OXYGEN ; CELL-LINE ; SUPF GENE ; DAMAGE ; MUTAGENICITY
英文摘要

Nitric oxide (NO) as well as its donors has been shown to generate mutation and DNA damage in in vitro assays. The objective of this study was to identify that DNA single-strand breaks (SSBs) could be elicited by NO, not only in vitro but also in vivo. The alkaline single-cell gel electrophoresis (SCGE) was performed to examine the DNA damage in g12 cells and the cells isolated from the organs of mice exposed to sodium nitroprusside (SNP). A modified method, in which neither collagenase nor trypsin was necessary, was used to prepare the single-cell suspension isolated from organs of mice. Results showed that the exposure of g12 cells to 0.13-0.5 mu mol/ml SNP with S9 for 1 h induced a concentration-dependent increase in DNA SSBs in g12 cells. The significant increase in DNA migration and comet frequency has appeared in the cells isolated from the spleen, thymus, and peritoneal macrophages of mice after injecting i.p. SNP in the dosage range of 0.67-6.0 mg/kg b.wt for 1 h. However, no obvious increase in DNA strand breaks was observed in the cells isolated from the liver, kidney, lung, brain and heart obtained from the same treated mice. These results suggested that DNA SSBs could be induced by NO in some cells both in vivo and in vitro. There were organ differences in sensitivity in the mice exposed to NO. Spleen, thymus, and macrophages might be the important targets of NO. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000086186500006
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53728
专题北京大学公共卫生学院_毒理学系
作者单位Beijing Med Univ, Dept Toxicol, Beijing 100083, Peoples R China
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GB/T 7714
Lin, WC,Wei, XT,Xue, HW,et al. Study on DNA strand breaks induced by sodium nitroprusside, a nitric oxide donor, in vivo and in vitro[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2000,466(2):187-195.
APA Lin, WC.,Wei, XT.,Xue, HW.,Kelimu, M.,Tao, R.,...&Zhou, ZC.(2000).Study on DNA strand breaks induced by sodium nitroprusside, a nitric oxide donor, in vivo and in vitro.MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,466(2),187-195.
MLA Lin, WC,et al."Study on DNA strand breaks induced by sodium nitroprusside, a nitric oxide donor, in vivo and in vitro".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 466.2(2000):187-195.
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