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学科主题: 公共卫生
题名:
Study on DNA strand breaks induced by sodium nitroprusside, a nitric oxide donor, in vivo and in vitro
作者: Lin, WC; Wei, XT; Xue, HW; Kelimu, M; Tao, R; Song, YS; Zhou, ZC
关键词: nitric oxide ; sodium nitroprusside ; DNA single-strand breaks ; in vitro ; in vivo
刊名: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
发表日期: 2000-03-23
卷: 466, 期:2, 页:187-195
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
关键词[WOS]: ELECTROPHORESIS COMET ASSAY ; SJL MICE ; IN-VIVO ; PEROXYNITRITE CAUSES ; MEDIATED APOPTOSIS ; REACTIVE OXYGEN ; CELL-LINE ; SUPF GENE ; DAMAGE ; MUTAGENICITY
英文摘要:

Nitric oxide (NO) as well as its donors has been shown to generate mutation and DNA damage in in vitro assays. The objective of this study was to identify that DNA single-strand breaks (SSBs) could be elicited by NO, not only in vitro but also in vivo. The alkaline single-cell gel electrophoresis (SCGE) was performed to examine the DNA damage in g12 cells and the cells isolated from the organs of mice exposed to sodium nitroprusside (SNP). A modified method, in which neither collagenase nor trypsin was necessary, was used to prepare the single-cell suspension isolated from organs of mice. Results showed that the exposure of g12 cells to 0.13-0.5 mu mol/ml SNP with S9 for 1 h induced a concentration-dependent increase in DNA SSBs in g12 cells. The significant increase in DNA migration and comet frequency has appeared in the cells isolated from the spleen, thymus, and peritoneal macrophages of mice after injecting i.p. SNP in the dosage range of 0.67-6.0 mg/kg b.wt for 1 h. However, no obvious increase in DNA strand breaks was observed in the cells isolated from the liver, kidney, lung, brain and heart obtained from the same treated mice. These results suggested that DNA SSBs could be induced by NO in some cells both in vivo and in vitro. There were organ differences in sensitivity in the mice exposed to NO. Spleen, thymus, and macrophages might be the important targets of NO. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000086186500006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53728
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: Beijing Med Univ, Dept Toxicol, Beijing 100083, Peoples R China

Recommended Citation:
Lin, WC,Wei, XT,Xue, HW,et al. Study on DNA strand breaks induced by sodium nitroprusside, a nitric oxide donor, in vivo and in vitro[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2000,466(2):187-195.
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