IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities
Liu, Xiaochen2; Zhao, Minzhi2; Lu, Jingxiong3; Ma, Jian4; Wei, Jie1,3; Wei, Shicheng1,2
关键词Nanohydroxyapatite Osteoblast-like Cells Cell Viability Cell Differentiation
刊名INTERNATIONAL JOURNAL OF NANOMEDICINE
2012
DOI10.2147/IJN.S28098
7页:1239-1250
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
资助者State Key Development Program for Basic Research of China ; Fundamental Research Funds for the Central Universities ; Peking University ; National Natural Science Foundation of China ; Key Medical Program of Science and Technology Development of Shanghai ; State Key Development Program for Basic Research of China ; Fundamental Research Funds for the Central Universities ; Peking University ; National Natural Science Foundation of China ; Key Medical Program of Science and Technology Development of Shanghai
研究领域[WOS]Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]IN-VITRO ; HYDROTHERMAL METHOD ; OSTEOBLAST PHENOTYPE ; HYDROXYAPATITE ; APOPTOSIS ; PROLIFERATION ; BIOCERAMICS ; TEMPERATURE ; DISSOLUTION ; COATINGS
英文摘要

Introduction: Hydroxyapatite (HA) is the principal inorganic constituent of human bone. Due to its good biocompatibility and osteoconductivity, all kinds of HA particles were prepared by different methods. Numerous reports demonstrated that the properties of HA affected its biological effects.

Methods: Two kinds of nanohydroxyapatite with different sizes and crystallinities were obtained via a hydrothermal treatment method under different temperatures. It was found that at a temperature of 140 C, a rod-like crystal (n-HA1) with a diameter of 23 +/- 5 nm, a length of 47 +/- 14 nm, and crystallinity of 85% +/- 5% was produced, while at a temperature of 80 degrees C, a -rod-like crystal (n-HA2) with a diameter of 16 +/- 3 nm, a length of 40 +/- 10 nm, and -crystallinity of 65% +/- 3% was produced. The influence of nanohydroxyapatite size and crystallinity on osteoblast viability was studied by MTT, scanning electron microscopy, and flow cytometry.

Results: n-HA1 gave a better biological response than n-HA2 in promoting cell growth and inhibiting cell apoptosis, and also exhibited much more active cell morphology. Alkaline phosphatase activity for both n-HA2 and n-HA1 was obviously higher than for the control, and no significant difference was found between n-HA1 and n-HA2. The same trend was observed on Western blotting for expression of type I collagen and osteopontin. In addition, it was found by transmission electron microscopy that large quantities of n-HA2 entered into the cell and damaged the cellular morphology. Release of tumor necrosis factor alpha from n-HA2 was markedly higher than from n-HA1, indicating that n-HA2 might trigger a severe inflammatory response.

Conclusion: This work indicates that not all nanohydroxyapatite should be considered a good biomaterial in future clinical applications.

语种英语
所属项目编号2007CB936103 ; 985 ; 30970720 ; 09411954900
资助者State Key Development Program for Basic Research of China ; Fundamental Research Funds for the Central Universities ; Peking University ; National Natural Science Foundation of China ; Key Medical Program of Science and Technology Development of Shanghai ; State Key Development Program for Basic Research of China ; Fundamental Research Funds for the Central Universities ; Peking University ; National Natural Science Foundation of China ; Key Medical Program of Science and Technology Development of Shanghai
WOS记录号WOS:000302724200001
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53729
Collection北京大学口腔医学院
作者单位1.Tongji Univ, Hosp Stomatol, Shanghai 200092, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
3.Peking Univ, Ctr Biomed Mat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100081, Peoples R China
4.E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xiaochen,Zhao, Minzhi,Lu, Jingxiong,et al. Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2012,7:1239-1250.
APA Liu, Xiaochen,Zhao, Minzhi,Lu, Jingxiong,Ma, Jian,Wei, Jie,&Wei, Shicheng.(2012).Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities.INTERNATIONAL JOURNAL OF NANOMEDICINE,7,1239-1250.
MLA Liu, Xiaochen,et al."Cell responses to two kinds of nanohydroxyapatite with different sizes and crystallinities".INTERNATIONAL JOURNAL OF NANOMEDICINE 7(2012):1239-1250.
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