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Production and secretion of calcitonin gene-related peptide from human lymphocytes
Wang, HC; Xing, LY; Li, WJ; Hou, LF; Guo, JX; Wang, X
关键词Cgrp Lymphocyte Pha Neuroimmune Modulation
刊名JOURNAL OF NEUROIMMUNOLOGY
2002-09-01
130期:1-2页:155-162
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Neurosciences
研究领域[WOS]Immunology ; Neurosciences & Neurology
关键词[WOS]CGRP MESSENGER-RNAS ; ALPHA-CGRP ; BETA-CGRP ; DIFFERENTIAL REGULATION ; LEUKOCYTE INTERFERON ; SPINAL-CORD ; EXPRESSION ; RAT ; NEUROPEPTIDES ; CELLS
英文摘要

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide, which is mainly present in primary sensory nerves. Although our previous study has shown that rat lymphocytes can synthesize beta-CGRP, there is no evidence demonstrating whether CGRP can be synthesized by human lymphocytes. In this study, the production of CGRP from human lymphocytes from spleen and blood were investigated by using CGRP-specific radioimmunoassay (RIA), and RNase protection assay (RPA). The results showed that human T lymphocyte mitogen, such as phytohemagglutinin (PHA), could time- and dose-dependently induce hCGRP secretion; rhIL-2 alone did not effect hCGRP secretion, but it could potentiate PHA-evoked hCGRP secretion from human spleen lymphocytes. RPA showed that alpha- and beta-CGRP mRNA were both constitutively expressed in unstimulated human peripheral blood mononuclear cells (PBMC). PHA could cause beta-hCGRP but not alpha-hCGRP mRNA increase in a time-dependent manner. In addition, hCGRP(8-37), a CGRP(1) receptor antagonist, enhanced PHA or human interleukin-2 (rhIL-2), induced the proliferation of splenocytes and PBMC. These results suggest that hCGRP is produced and secreted by human lymphocyte. Lymphocyte mitogen can induce the elevation of beta-CGRP synthesis and secretion. The lymphocyte-derived beta-CGRP may inhibit, at least in part, lymphocytes proliferation, which are then involved in the modulation of human T lymphocyte function in response to immune stimulation. (C) 2002 Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000178329200015
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被引频次:40[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53737
专题北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
2.Peking Univ, Minist Mol Cardiol, Reference Lab Educ, Beijing 100083, Peoples R China
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Wang, HC,Xing, LY,Li, WJ,et al. Production and secretion of calcitonin gene-related peptide from human lymphocytes[J]. JOURNAL OF NEUROIMMUNOLOGY,2002,130(1-2):155-162.
APA Wang, HC,Xing, LY,Li, WJ,Hou, LF,Guo, JX,&Wang, X.(2002).Production and secretion of calcitonin gene-related peptide from human lymphocytes.JOURNAL OF NEUROIMMUNOLOGY,130(1-2),155-162.
MLA Wang, HC,et al."Production and secretion of calcitonin gene-related peptide from human lymphocytes".JOURNAL OF NEUROIMMUNOLOGY 130.1-2(2002):155-162.
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