IR@PKUHSC  > 北京大学第二临床医学院  > 心血管内科
学科主题临床医学
MicroRNA-19b functions as potential anti-thrombotic protector in patients with unstable angina by targeting tissue factor
Li, Sufang1; Ren, Jingyi1; Xu, Ning2; Zhang, Jing1; Geng, Qiang1; Cao, Chengfu1; Lee, Chongyou1; Song, Junxian1; Li, Jingjin1; Chen, Hong1
关键词Unstable Angina Thrombosis Endothelial Cells Microparticles Mirnas Tissue Factor
刊名JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
2014-10-01
DOI10.1016/j.yjmcc.2014.06.017
75页:49-57
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems ; Cell Biology
研究领域[WOS]Cardiovascular System & Cardiology ; Cell Biology
关键词[WOS]CIRCULATING MICRORNAS ; CARDIOVASCULAR-DISEASES ; MYOCARDIAL-INFARCTION ; CORONARY-THROMBOSIS ; FACTOR EXPRESSION ; MECHANISMS ; MICROPARTICLES ; BIOMARKERS ; ATHEROTHROMBOSIS ; RECOGNITION
英文摘要

The activation of a hemostatic system plays a critical role in the incidence of acute coronary events. Hemostatic proteins may be regulated by microRNAs (miRNAs). Microparticles (MPs) are the major carrier of circulating miRNAs. The aim of this study was to determine the potential role of miRNAs in regulating gene expression involved in the hemostatic system in patients with unstable angina (UA). MiRNA expression profiles in the plasma from patients with UA (UA group, n = 9) compared with individuals with clinical suspicion of coronary artery disease (CAD) but negative angiography (control group, n = 9) showed that among 36 differentially expressed miRNAs, miR-19b was the most obvious one. Using real-time PCR, 5 selected miRNA levels in plasma (UA group, n = 20; control group, n = 30) and plasma MPs (UA group n = 6; control group n = 6) were proved to be consistent with the miRNA array. Flow cytometry analysis indicated that the amounts of plasma endothelial microparticles (EMPs) were increased in UA patients (UA group, n = 4) compared to controls (control group, n = 4). In cultured endothelial cells (ECs), TNIF-alpha increased miR-19b release and expression. Tissue factor (TF) was predicted to be the target of miR-19b by bioinformatics analysis. Luciferase reporter assays demonstrated that miR-19b binds to TF mRNA. Overexpression of miR-19b inhibited TF expression and procoagulant activity. This study indicates that in UA patients, the increase of miR-19b wrapped in EMPs due to endothelial dysfunction may partially contribute to the circulating miR-19b elevation and miR-19b may play an anti-thrombotic role by inhibiting the expression of TF in ECs. (C) 2014 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000342271700005
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53750
专题北京大学第二临床医学院_心血管内科
作者单位1.Karolinska Inst, Dept Med, Stockholm, Sweden
2.Peking Univ, Peoples Hosp, Dept Cardiol, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Li, Sufang,Ren, Jingyi,Xu, Ning,et al. MicroRNA-19b functions as potential anti-thrombotic protector in patients with unstable angina by targeting tissue factor[J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY,2014,75:49-57.
APA Li, Sufang.,Ren, Jingyi.,Xu, Ning.,Zhang, Jing.,Geng, Qiang.,...&Chen, Hong.(2014).MicroRNA-19b functions as potential anti-thrombotic protector in patients with unstable angina by targeting tissue factor.JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY,75,49-57.
MLA Li, Sufang,et al."MicroRNA-19b functions as potential anti-thrombotic protector in patients with unstable angina by targeting tissue factor".JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY 75(2014):49-57.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Sufang]的文章
[Ren, Jingyi]的文章
[Xu, Ning]的文章
百度学术
百度学术中相似的文章
[Li, Sufang]的文章
[Ren, Jingyi]的文章
[Xu, Ning]的文章
必应学术
必应学术中相似的文章
[Li, Sufang]的文章
[Ren, Jingyi]的文章
[Xu, Ning]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。