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IR@PKUHSC  > 北京大学第二临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
MicroRNA-19b functions as potential anti-thrombotic protector in patients with unstable angina by targeting tissue factor
作者: Li, Sufang1; Ren, Jingyi1; Xu, Ning2; Zhang, Jing1; Geng, Qiang1; Cao, Chengfu1; Lee, Chongyou1; Song, Junxian1; Li, Jingjin1; Chen, Hong1
关键词: Unstable angina ; Thrombosis ; Endothelial cells ; Microparticles ; miRNAs ; Tissue factor
刊名: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
发表日期: 2014-10-01
DOI: 10.1016/j.yjmcc.2014.06.017
卷: 75, 页:49-57
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cardiac & Cardiovascular Systems ; Cell Biology
研究领域[WOS]: Cardiovascular System & Cardiology ; Cell Biology
关键词[WOS]: CIRCULATING MICRORNAS ; CARDIOVASCULAR-DISEASES ; MYOCARDIAL-INFARCTION ; CORONARY-THROMBOSIS ; FACTOR EXPRESSION ; MECHANISMS ; MICROPARTICLES ; BIOMARKERS ; ATHEROTHROMBOSIS ; RECOGNITION
英文摘要:

The activation of a hemostatic system plays a critical role in the incidence of acute coronary events. Hemostatic proteins may be regulated by microRNAs (miRNAs). Microparticles (MPs) are the major carrier of circulating miRNAs. The aim of this study was to determine the potential role of miRNAs in regulating gene expression involved in the hemostatic system in patients with unstable angina (UA). MiRNA expression profiles in the plasma from patients with UA (UA group, n = 9) compared with individuals with clinical suspicion of coronary artery disease (CAD) but negative angiography (control group, n = 9) showed that among 36 differentially expressed miRNAs, miR-19b was the most obvious one. Using real-time PCR, 5 selected miRNA levels in plasma (UA group, n = 20; control group, n = 30) and plasma MPs (UA group n = 6; control group n = 6) were proved to be consistent with the miRNA array. Flow cytometry analysis indicated that the amounts of plasma endothelial microparticles (EMPs) were increased in UA patients (UA group, n = 4) compared to controls (control group, n = 4). In cultured endothelial cells (ECs), TNIF-alpha increased miR-19b release and expression. Tissue factor (TF) was predicted to be the target of miR-19b by bioinformatics analysis. Luciferase reporter assays demonstrated that miR-19b binds to TF mRNA. Overexpression of miR-19b inhibited TF expression and procoagulant activity. This study indicates that in UA patients, the increase of miR-19b wrapped in EMPs due to endothelial dysfunction may partially contribute to the circulating miR-19b elevation and miR-19b may play an anti-thrombotic role by inhibiting the expression of TF in ECs. (C) 2014 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2012ZX09303019 ; 81070179 ; 81270274 ; 7132225 ; 7122198 ; 2011-4022-03
项目资助者: National Science and Technology "Creation of Major New Dugs" ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Capital Health Research and Development of Special
WOS记录号: WOS:000342271700005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53750
Appears in Collections:北京大学第二临床医学院_心血管内科_期刊论文

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作者单位: 1.Karolinska Inst, Dept Med, Stockholm, Sweden
2.Peking Univ, Peoples Hosp, Dept Cardiol, Beijing 100044, Peoples R China

Recommended Citation:
Li, Sufang,Ren, Jingyi,Xu, Ning,et al. MicroRNA-19b functions as potential anti-thrombotic protector in patients with unstable angina by targeting tissue factor[J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY,2014,75:49-57.
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