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学科主题: 临床医学
题名:
Anti-Proliferative Efficacy of Icariin on HepG2 Hepatoma and Its Possible Mechanism of Action
作者: Yang, Jin-Xia1,2; Fichtner, Iduna3; Becker, Monika3; Lemm, Margit3; Wang, Xue-Mei1,2
关键词: Icariin ; HepG2 ; CD31 ; Ki67 ; Flow Cytometry ; MTT Assay ; Immunohistochemical Assay
刊名: AMERICAN JOURNAL OF CHINESE MEDICINE
发表日期: 2009
DOI: 10.1142/S0192415X09007569
卷: 37, 期:6, 页:1153-1165
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Integrative & Complementary Medicine ; Medicine, General & Internal
研究领域[WOS]: Integrative & Complementary Medicine ; General & Internal Medicine
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; ENDOTHELIAL-CELLS ; IN-VITRO ; DIFFERENTIATION ; ANGIOGENESIS ; EXPRESSION ; GLYCOSIDES ; GROWTH ; TUMORS ; RATS
英文摘要:

The aim of the present work was to explore the anti-hepatoma effects of icariin both in vitro and in vivo and to elucidate its potential mechanism of action. The MTT assay was applied to test the anti-proliferative effects of icariin in vitro. HepG2 bearing NMRI nu/nu mice were used to test the anticancer effects of icariin in vivo. Immunohistochemical assay and flow cytometry assay (FACS) were applied to detect the possible mechanisms of action of icariin. MTT assay illustrated that icariin inhibited the proliferation of HepG2 cells in a concentration dependent manner; meanwhile, icariin inhibited the tumor growth in HepG2 bearing NMRI nu/nu mice. The tumor weight was inhibited by 55.6% and tumor volume was inhibited by 47.2%. Icariin did not influence the spleen and body weights or blood parameters. Immunohistochemical analysis indicated that the expressions of both CD31 and Ki67 in the icariin treated group were significantly lower than those in the control group (p < 0.01). FACS assay showed that icariin dramatically decreased the percentage of CD4+ and CD8+ cells in bone marrow and CD19+ cells in blood on day 8. On day 17, the percentage of CD8+ cells in blood was lower than those in the control group. CD4/CD8 ratio in icariin group was significantly elevated in bone marrow on day 17. Icariin showed anticancer efficacy both in vitro and in vivo. The possible mechanism of action could be related to its anti-angiogenesis and anti-proliferative effects in tumors.

语种: 英语
项目资助者: Experimental Pharmacology and Oncology GmbH (EPO)
WOS记录号: WOS:000272093600012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53767
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Integrated Lab TCM & Western Med, Beijing 100034, Peoples R China
2.Peking Univ, Integrated Inst TCM & Western Med, Beijing 100034, Peoples R China
3.Expt Pharmacol Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany

Recommended Citation:
Yang, Jin-Xia,Fichtner, Iduna,Becker, Monika,et al. Anti-Proliferative Efficacy of Icariin on HepG2 Hepatoma and Its Possible Mechanism of Action[J]. AMERICAN JOURNAL OF CHINESE MEDICINE,2009,37(6):1153-1165.
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