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学科主题: 基础医学
题名:
Klotho is a target gene of PPAR-gamma
作者: Zhang, Hong1,2; Li, Yuanyuan1; Fan, Yanbo1; Wu, Jing3; Zhao, Beilei1; Guan, Youfei2,3; Chien, Shu4; Wang, Nanping1,2
关键词: gene expression ; transcription factor ; diabetic nephropathy
刊名: KIDNEY INTERNATIONAL
发表日期: 2008-09-01
DOI: 10.1038/ki.2008.244
卷: 74, 期:6, 页:732-739
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: PROLIFERATOR-ACTIVATED RECEPTORS ; VASCULAR ENDOTHELIAL-CELLS ; RENAL-FAILURE ; C-JUN ; KIDNEY ; THIAZOLIDINEDIONES ; EXPRESSION ; MICE ; LINE ; METABOLISM
英文摘要:

Klotho is an anti-aging gene whose expression is regulated by many stimuli. Here we examined the transcriptional regulation of the klotho gene by peroxisome proliferator-activated receptor-gamma (PPAR-gamma). The PPAR-gamma agonists thiazolidinediones increased both klotho mRNA and protein expression in HEK293 cells and several renal epithelial cell lines. The induction was blocked by PPAR-gamma antagonists or small-interfering RNA-mediated gene silencing of PPAR-gamma, suggesting a PPAR-gamma-dependent mechanism. Chromatin immuno-precipitation and gel shift assays found a noncanonical PPAR-responsive element within the 5 ′-flanking region of the human klotho gene with promoter-reporter assays further confirming transcriptional functionality. Moreover, thiazolidinediones or adenovirus-mediated overexpression of PPAR-gamma increased klotho expression in mouse kidneys while renal klotho expression was attenuated in mice treated with PPAR-gamma antagonists. These results demonstrate that klotho is a target gene of PPAR-gamma.

语种: 英语
所属项目编号: 30470810 ; 30670848 ; 30600231 ; 2006CB503900 ; 2006CB943503
项目资助者: Ministry of Education (PCSIRT) ; National Science Foundation of China ; Ministry of Science and Technology
WOS记录号: WOS:000258874600009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53769
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Ctr Diabet, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
4.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA

Recommended Citation:
Zhang, Hong,Li, Yuanyuan,Fan, Yanbo,et al. Klotho is a target gene of PPAR-gamma[J]. KIDNEY INTERNATIONAL,2008,74(6):732-739.
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