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学科主题: 临床医学
题名:
Complete genome sequence of methicillin-sensitive Staphylococcus aureus containing a heterogeneic staphylococcal cassette chromosome element
作者: Li DeZhi1,2; Chu YaNan3,4; Ren LuFeng3,4; Li XinGang3,4; Yuan Lina3,4; Kang Yu3; Zhang Wei5; Yang Yu6; Wang XuMin3; Baillie, J. Kenneth7,8; Yu Jun3,4; Gao ZhanCheng1
关键词: Staphylococcus aureus ; staphylococcal cassette chromosome mec (SCC-mec) ; genomics ; pyrosequencing
刊名: SCIENCE CHINA-LIFE SCIENCES
发表日期: 2013-03-01
DOI: 10.1007/s11427-013-4453-9
卷: 56, 期:3, 页:268-274
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: RESISTANCE ; EVOLUTION ; MEC ; GENES ; HOST ; INFECTION ; VIRULENCE ; INVASION ; STRAINS
英文摘要:

Staphylococcus aureus is a common human bacterium that sometimes becomes pathogenic, causing serious infections. A key feature of S. aureus is its ability to acquire resistance to antibiotics. The presence of the staphylococcal cassette chromosome (SCC) element in serotypes of S. aureus has been confirmed using multiplex PCR assays. The SCC element is the only vector known to carry the mecA gene, which encodes methicillin resistance in S. aureus infections. Here, we report the genome sequence of a novel methicillin-sensitive S. aureus (MSSA) strain: SCC-like MSSA463. This strain was originally erroneously serotyped as methicillin-resistant S. aureus in a clinical laboratory using multiplex PCR methods. We sequenced the genome of SCC-like MSSA463 using pyrosequencing techniques and compared it with known genome sequences of other S. aureus isolates. An open reading frame (CZ049; AB037671) was identified downstream of attL and attR inverted repeat sequences. Our results suggest that a lateral gene transfer occurred between S. aureus and other organisms, partially changing S. aureus infectivity. We propose that attL and attR inverted repeats in S. aureus serve as frequent insertion sites for exogenous genes.

语种: 英语
所属项目编号: 2006AA02Z4A9 ; 2009ZX10004 ; 2012ZX10004206 ; 30971610 ; 30900053
项目资助者: National High Technology Research and Development Program ; National Science and Technology Major Project of Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Young Scientist Innovation Promotion Association of the Chinese Academy of Sciences
WOS记录号: WOS:000316747000011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53788
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China
2.Shandong Univ, Shandong Prov Hosp, Dept Resp Med, Jinan 250021, Peoples R China
3.Chinese Acad Sci, Beijing Inst Genom, CAS Key Lab Genome Sci & Informat, Beijing 100029, Peoples R China
4.Chinese Acad Sci, Beijing Inst Genom, DNA Sequencing Technol R&D Ctr, Beijing 100029, Peoples R China
5.Nanchang Univ, Affiliated Hosp 1, Dept Resp Med, Nanchang 330006, Peoples R China
6.Chinese Acad Inspect & Quarantine, Beijing 100025, Peoples R China
7.Univ Edinburgh, Roslin Inst, Div Genet & Genom, Roslin EH25 9RG, Midlothian, Scotland
8.Univ Edinburgh, Dept Crit Care Med, Edinburgh EH16 4TJ, Midlothian, Scotland

Recommended Citation:
Li DeZhi,Chu YaNan,Ren LuFeng,et al. Complete genome sequence of methicillin-sensitive Staphylococcus aureus containing a heterogeneic staphylococcal cassette chromosome element[J]. SCIENCE CHINA-LIFE SCIENCES,2013,56(3):268-274.
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