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学科主题口腔医学
Inhibition of mechanical stress-induced NF-kappa B promotes bone formation
Zhou, S.1; Zhang, J.1; Zheng, H.1; Zhou, Y.1; Chen, F.2; Lin, J.1
关键词Nuclear Factor Kappab Compressive Force Osteoblast Bone Formation
刊名ORAL DISEASES
2013
DOI10.1111/j.1601-0825.2012.01949.x
19期:1页:59-64
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]MESENCHYMAL STEM-CELLS ; EXPRESSION ; PHOSPHORYLATION ; DIFFERENTIATION ; OSTEOBLASTS ; DISEASE
英文摘要

OBJECTIVE: Nuclear factor kappaB (NF-?B) plays an important role in osteogenesis. This study was performed to investigate the effects of mechanical force on NF-?B activity in osteoblast-like cells.

Materials and methods: U2OS cells were harvested at specific time points after mechanical loading. U2OS nuclear extracts were prepared for Western blot assay and electrophoretic mobility shift assay electrophoretic mobility shift assay (EMSA). Total RNA was isolated using TRIzol for real-time PCR.

Results: P-p65 (Ser536) expression in the nucleus was significantly higher after loading force on U2OS cells. The amount of nuclear NF-?B also increased. Ammonium pyrrolidinedithiocarbamate(PDCT) inhibited compressive force-induced NF-?B activity in EMSA. Further, PDTC attenuated the transcriptional inhibition of alkaline phosphatase (ALP), RUNX-2 and Osteocalcin by down-regulating NF-?B activity.

Conclusions: Mechanical force enhances NF-?B activity in osteoblast-like cells, and compressive force affects the downstream bone marker genes through NF-?B. Oral Diseases (2012) 19, 5964

语种英语
WOS记录号WOS:000312247100005
项目编号PKUSS20110301
资助机构Peking University School of Stomatology
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53789
专题北京大学口腔医学院_牙周科
北京大学临床肿瘤学院_妇科肿瘤科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100871, Peoples R China
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Zhou, S.,Zhang, J.,Zheng, H.,et al. Inhibition of mechanical stress-induced NF-kappa B promotes bone formation[J]. ORAL DISEASES,2013,19(1):59-64.
APA Zhou, S.,Zhang, J.,Zheng, H.,Zhou, Y.,Chen, F.,&Lin, J..(2013).Inhibition of mechanical stress-induced NF-kappa B promotes bone formation.ORAL DISEASES,19(1),59-64.
MLA Zhou, S.,et al."Inhibition of mechanical stress-induced NF-kappa B promotes bone formation".ORAL DISEASES 19.1(2013):59-64.
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