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学科主题: 口腔医学
题名:
Inhibition of mechanical stress-induced NF-kappa B promotes bone formation
作者: Zhou, S.1; Zhang, J.1; Zheng, H.1; Zhou, Y.1; Chen, F.2; Lin, J.1
关键词: nuclear factor kappaB ; compressive force ; osteoblast ; bone formation
刊名: ORAL DISEASES
发表日期: 2013
DOI: 10.1111/j.1601-0825.2012.01949.x
卷: 19, 期:1, 页:59-64
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: MESENCHYMAL STEM-CELLS ; EXPRESSION ; PHOSPHORYLATION ; DIFFERENTIATION ; OSTEOBLASTS ; DISEASE
英文摘要:

OBJECTIVE: Nuclear factor kappaB (NF-?B) plays an important role in osteogenesis. This study was performed to investigate the effects of mechanical force on NF-?B activity in osteoblast-like cells.

Materials and methods: U2OS cells were harvested at specific time points after mechanical loading. U2OS nuclear extracts were prepared for Western blot assay and electrophoretic mobility shift assay electrophoretic mobility shift assay (EMSA). Total RNA was isolated using TRIzol for real-time PCR.

Results: P-p65 (Ser536) expression in the nucleus was significantly higher after loading force on U2OS cells. The amount of nuclear NF-?B also increased. Ammonium pyrrolidinedithiocarbamate(PDCT) inhibited compressive force-induced NF-?B activity in EMSA. Further, PDTC attenuated the transcriptional inhibition of alkaline phosphatase (ALP), RUNX-2 and Osteocalcin by down-regulating NF-?B activity.

Conclusions: Mechanical force enhances NF-?B activity in osteoblast-like cells, and compressive force affects the downstream bone marker genes through NF-?B. Oral Diseases (2012) 19, 5964

语种: 英语
所属项目编号: PKUSS20110301
项目资助者: Peking University School of Stomatology
WOS记录号: WOS:000312247100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53789
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100871, Peoples R China

Recommended Citation:
Zhou, S.,Zhang, J.,Zheng, H.,et al. Inhibition of mechanical stress-induced NF-kappa B promotes bone formation[J]. ORAL DISEASES,2013,19(1):59-64.
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