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学科主题: 基础医学
题名:
The thymic stromal cell line MTSC4 induced thymocyte apoptosis in a non-MHC-restricted manner
作者: He, XY; Li, J; Qian, XP; Fu, WX; Li, Y; Wu, L; Chen, WF
关键词: MHC ; thymic dendritic cells ; thymic stromal cell line ; thymocyte apoptosis
刊名: CELL RESEARCH
发表日期: 2004-04-01
卷: 14, 期:2, 页:125-133
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: NEGATIVE SELECTION ; DENDRITIC CELLS ; IN-VIVO ; EXPRESSION ; MOLECULES ; REPERTOIRE ; INDUCTION ; PEPTIDE ; FAS ; TCR
英文摘要:

Mouse thymic stromal cell line 4 (MTSC4) is one of the stromal cell lines established in our laboratory. While losing the characteristics of epithelial cells, they express some surface markers shared with thymic dendritic cells (TDCs). To further study the biological functions of these cells, we compared the capability of MTSC4 with TDCs in the induction of thymocyte apoptosis, using thymic reaggregation culture system. Apoptosis of thymocytes induced by MTSC4 and TDCs was measured by Annexin V and PI staining and analyzed by flow cytometry. We found that MTSC4 selectively augmented the apoptosis of CD4(+)8(+) (DP) thymocytes. This effect was Fas/FasL independent and could not be blocked by antibodies to MHC class I and class 11 molecules. In addition, MTSC4 enhanced the apoptosis of DP thymocytes from different strains of mice, which implies that MTSC4-induced thymocyte apoptosis is not mediated by the TCR recognition of self peptide/MHC molecules. In contrast to MTSC4, thymocyte apoptosis induced by TDCs was MHC-restricted. Thus, MHC-independent fashion of stromal-DP thymocyte interaction may be one of the ways to induce thymocyte apoptosis in thymus. Our study has also shown that the interaction of MTSC4 stromal cells and thymocytes is required for the induction of thymocyte apoptosis.

语种: 英语
WOS记录号: WOS:000221250900004
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53790
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Ctr Hlth Sci, Dept Immunol, Beijing 100083, Peoples R China
2.PO Royal Melbourne Hosp, Walter & Eliza Hall Inst Med Res, Melbourne, Vic 3050, Australia

Recommended Citation:
He, XY,Li, J,Qian, XP,et al. The thymic stromal cell line MTSC4 induced thymocyte apoptosis in a non-MHC-restricted manner[J]. CELL RESEARCH,2004,14(2):125-133.
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