北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科  > 期刊论文
学科主题: 临床医学
题名:
Influence of myeloperoxidase-catalyzing reaction on the binding between myeloperoxidase and anti-myeloperoxidase antibodies
作者: Xu, Peng-Cheng; Hao, Jian; Chen, Min; Cui, Zhao; Zhao, Ming-Hui
关键词: Antineutrophil cytoplasmic antibodies ; Myeloperoxidase ; Ceruloplasmin ; Catalysis
刊名: HUMAN IMMUNOLOGY
发表日期: 2012-04-01
DOI: 10.1016/j.humimm.2012.02.004
卷: 73, 期:4, 页:364-369
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: MICROSCOPIC POLYANGIITIS ; HUMAN-NEUTROPHILS ; REDOX PROPERTIES ; CATALYSIS ; AUTOANTIBODIES ; PROPYLTHIOURACIL ; INACTIVATION ; INHIBITION ; VASCULITIS ; ENZYME
英文摘要:

In the current study, whether myeloperoxidase (MPO)-catalyzing reaction could influence the antigenicity of MPO was investigated. Hypochlorite acid, the main product of the catalytic reaction, could lower the binding between MPO-antineutrophil cytoplasmic antibodies (ANCA) and MPO when the available chlorine was higher than 0.031 x 10(-3) g/l. After MPO-catalyzing reaction with H2O2 lower than 0.469 g/l, the binding level between MPO-ANCAcontaining plasma and MPO increased slightly. The peak binding level was 1.135 +/- 0.205 (expressed by the absorbance value at 405 nm). However, with the existence of hydrogen donor (o-phenylenediamine) in the reaction system, the peak binding level between MPO-ANCA-containing plasma and post-catalyzing MPO was significantly higher (1.367 +/- 0.321 vs 1.135 +/- 0.205, p = 0.023). Moreover, at the approximately physical concentration of H2O2 (0.02 g/l), MPO-ANCA exhibited higher titer to post-catalyzing MPO than to pre-catalyzing MPO (3.91 +/- 0.84 vs 3.57 +/- 0.84, p < 0.001, expressed as the IgT). These data demonstrated that MPO-catalyzing reaction could potentially increase the antigenicity of MPO. (C) 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 2012CB517702 ; 30972733 ; 81021004
项目资助者: Chinese 973 project ; National Natural Science Fund
WOS记录号: WOS:000302986200007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53791
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Key Lab Renal Dis, Minist Hlth China, Div Renal,Dept Med,Inst Nephrol,Hosp 1, Beijing 100871, Peoples R China

Recommended Citation:
Xu, Peng-Cheng,Hao, Jian,Chen, Min,et al. Influence of myeloperoxidase-catalyzing reaction on the binding between myeloperoxidase and anti-myeloperoxidase antibodies[J]. HUMAN IMMUNOLOGY,2012,73(4):364-369.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xu, Peng-Cheng]'s Articles
[Hao, Jian]'s Articles
[Chen, Min]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xu, Peng-Cheng]‘s Articles
[Hao, Jian]‘s Articles
[Chen, Min]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace