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Influence of myeloperoxidase-catalyzing reaction on the binding between myeloperoxidase and anti-myeloperoxidase antibodies
Xu, Peng-Cheng; Hao, Jian; Chen, Min; Cui, Zhao; Zhao, Ming-Hui
关键词Antineutrophil Cytoplasmic Antibodies Myeloperoxidase Ceruloplasmin Catalysis
刊名HUMAN IMMUNOLOGY
2012-04-01
DOI10.1016/j.humimm.2012.02.004
73期:4页:364-369
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]MICROSCOPIC POLYANGIITIS ; HUMAN-NEUTROPHILS ; REDOX PROPERTIES ; CATALYSIS ; AUTOANTIBODIES ; PROPYLTHIOURACIL ; INACTIVATION ; INHIBITION ; VASCULITIS ; ENZYME
英文摘要

In the current study, whether myeloperoxidase (MPO)-catalyzing reaction could influence the antigenicity of MPO was investigated. Hypochlorite acid, the main product of the catalytic reaction, could lower the binding between MPO-antineutrophil cytoplasmic antibodies (ANCA) and MPO when the available chlorine was higher than 0.031 x 10(-3) g/l. After MPO-catalyzing reaction with H2O2 lower than 0.469 g/l, the binding level between MPO-ANCAcontaining plasma and MPO increased slightly. The peak binding level was 1.135 +/- 0.205 (expressed by the absorbance value at 405 nm). However, with the existence of hydrogen donor (o-phenylenediamine) in the reaction system, the peak binding level between MPO-ANCA-containing plasma and post-catalyzing MPO was significantly higher (1.367 +/- 0.321 vs 1.135 +/- 0.205, p = 0.023). Moreover, at the approximately physical concentration of H2O2 (0.02 g/l), MPO-ANCA exhibited higher titer to post-catalyzing MPO than to pre-catalyzing MPO (3.91 +/- 0.84 vs 3.57 +/- 0.84, p < 0.001, expressed as the IgT). These data demonstrated that MPO-catalyzing reaction could potentially increase the antigenicity of MPO. (C) 2012 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000302986200007
项目编号2012CB517702 ; 30972733 ; 81021004
资助机构Chinese 973 project ; National Natural Science Fund
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53791
专题北京大学第一临床医学院_肾脏内科
作者单位Peking Univ, Key Lab Renal Dis, Minist Hlth China, Div Renal,Dept Med,Inst Nephrol,Hosp 1, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Xu, Peng-Cheng,Hao, Jian,Chen, Min,et al. Influence of myeloperoxidase-catalyzing reaction on the binding between myeloperoxidase and anti-myeloperoxidase antibodies[J]. HUMAN IMMUNOLOGY,2012,73(4):364-369.
APA Xu, Peng-Cheng,Hao, Jian,Chen, Min,Cui, Zhao,&Zhao, Ming-Hui.(2012).Influence of myeloperoxidase-catalyzing reaction on the binding between myeloperoxidase and anti-myeloperoxidase antibodies.HUMAN IMMUNOLOGY,73(4),364-369.
MLA Xu, Peng-Cheng,et al."Influence of myeloperoxidase-catalyzing reaction on the binding between myeloperoxidase and anti-myeloperoxidase antibodies".HUMAN IMMUNOLOGY 73.4(2012):364-369.
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