IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
The role of caveolin-1 and syndecan-4 in the internalization of PEGylated PAMAM dendrimer polyplexes into myoblast and hepatic cells
Shen, Wenwen1,2; van Dongen, Mallory A.3; Han, Yingchun1,2; Yu, Maomao1,2; Li, Yanzhi1,2; Liu, George1,2; Holl, Mark M. Banaszak3; Qi, Rong1,2
关键词Peg-pamam Dendrimer Polyplex Hepg2 C2c12 Endocytosis Mechanism Syndecan-4 Caveolin-1
刊名EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
2014-11-01
DOI10.1016/j.ejpb.2014.07.010
88期:3页:658-663
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]HEPARAN-SULFATE PROTEOGLYCANS ; GENE DELIVERY ; MEDIATED ENDOCYTOSIS ; SIRNA DELIVERY ; FAMILY ; POLYETHYLENIMINE ; ATHEROSCLEROSIS ; CYTOTOXICITY ; POLYMORPHISM ; LIPOPLEXES
英文摘要

To improve gene delivery efficiency of PEGylated poly(amidoamine) dendrimers in livers and muscles, the roles of syndecan-4 receptor and caveolin-1 protein in the endocytosis of PEGylated generation 5 (G5-PEG) or 7 (G7-PEG) dendrimers and plasmid DNA polyplexes were explored in C2C12 and HepG2 cells. Expression levels of syndecan-4 for both cell lines were downregulated by transfection of the cells with syndecan-4 specific siRNA. Caveolin-1 was upregulated by infecting the cells with adenovirus vector expressed caveolin-1 (Ad-CAV-1). The impact of syndecan-4 and caveolin-1 on endocytosis of G5-PEG/DNA or G7-PEG/DNA polyplexes was then measured by flow cytometry. Our results demonstrate that downregulation of syndecan-4 and upregulation of caveolin-1 significantly improved internalization of PEG-PAMAM dendrimer polyplexes in HepG2 cells; however, in C2C12 cells, downregulation of syndecan-4 decreased the internalization of the polyplexes while upregulation of caveolin-1 had no effect on internalization. Gene expression results for G5-PEG/pGFP on the two cell lines exhibited the same trends for syndecan-4 and caveolin-1 as was observed for endocytosis of the polyplexes. This study gives a clue how to take strategies by up- or down-regulation of the expressions of syndecan-4 and caveolin-1 to improve in vivo gene delivery efficiency of the PEG-PAMAM dendrimers in clinical transgenic therapy. (C) 2014 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000346951200010
项目编号30971241 ; 81270368 ; RO1-EB005028
资助机构National Natural Science Foundation of China ; federal funds from the National Institute of Biomedical Imaging and Bioengineering of the U.S.A.
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53805
专题北京大学基础医学院_心血管所
北京大学基础医学院
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
3.Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA
推荐引用方式
GB/T 7714
Shen, Wenwen,van Dongen, Mallory A.,Han, Yingchun,et al. The role of caveolin-1 and syndecan-4 in the internalization of PEGylated PAMAM dendrimer polyplexes into myoblast and hepatic cells[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2014,88(3):658-663.
APA Shen, Wenwen.,van Dongen, Mallory A..,Han, Yingchun.,Yu, Maomao.,Li, Yanzhi.,...&Qi, Rong.(2014).The role of caveolin-1 and syndecan-4 in the internalization of PEGylated PAMAM dendrimer polyplexes into myoblast and hepatic cells.EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,88(3),658-663.
MLA Shen, Wenwen,et al."The role of caveolin-1 and syndecan-4 in the internalization of PEGylated PAMAM dendrimer polyplexes into myoblast and hepatic cells".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 88.3(2014):658-663.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
1-s2.0-S093964111400(995KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Shen, Wenwen]的文章
[van Dongen, Mallory A.]的文章
[Han, Yingchun]的文章
百度学术
百度学术中相似的文章
[Shen, Wenwen]的文章
[van Dongen, Mallory A.]的文章
[Han, Yingchun]的文章
必应学术
必应学术中相似的文章
[Shen, Wenwen]的文章
[van Dongen, Mallory A.]的文章
[Han, Yingchun]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1-s2.0-S0939641114002355-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。