IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
The role of caveolin-1 and syndecan-4 in the internalization of PEGylated PAMAM dendrimer polyplexes into myoblast and hepatic cells
Shen, Wenwen1,2; van Dongen, Mallory A.3; Han, Yingchun1,2; Yu, Maomao1,2; Li, Yanzhi1,2; Liu, George1,2; Holl, Mark M. Banaszak3; Qi, Rong1,2
关键词Peg-pamam Dendrimer Polyplex Hepg2 C2c12 Endocytosis Mechanism Syndecan-4 Caveolin-1
刊名EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
2014-11-01
DOI10.1016/j.ejpb.2014.07.010
88期:3页:658-663
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
资助者National Natural Science Foundation of China ; federal funds from the National Institute of Biomedical Imaging and Bioengineering of the U.S.A. ; National Natural Science Foundation of China ; federal funds from the National Institute of Biomedical Imaging and Bioengineering of the U.S.A.
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]HEPARAN-SULFATE PROTEOGLYCANS ; GENE DELIVERY ; MEDIATED ENDOCYTOSIS ; SIRNA DELIVERY ; FAMILY ; POLYETHYLENIMINE ; ATHEROSCLEROSIS ; CYTOTOXICITY ; POLYMORPHISM ; LIPOPLEXES
英文摘要

To improve gene delivery efficiency of PEGylated poly(amidoamine) dendrimers in livers and muscles, the roles of syndecan-4 receptor and caveolin-1 protein in the endocytosis of PEGylated generation 5 (G5-PEG) or 7 (G7-PEG) dendrimers and plasmid DNA polyplexes were explored in C2C12 and HepG2 cells. Expression levels of syndecan-4 for both cell lines were downregulated by transfection of the cells with syndecan-4 specific siRNA. Caveolin-1 was upregulated by infecting the cells with adenovirus vector expressed caveolin-1 (Ad-CAV-1). The impact of syndecan-4 and caveolin-1 on endocytosis of G5-PEG/DNA or G7-PEG/DNA polyplexes was then measured by flow cytometry. Our results demonstrate that downregulation of syndecan-4 and upregulation of caveolin-1 significantly improved internalization of PEG-PAMAM dendrimer polyplexes in HepG2 cells; however, in C2C12 cells, downregulation of syndecan-4 decreased the internalization of the polyplexes while upregulation of caveolin-1 had no effect on internalization. Gene expression results for G5-PEG/pGFP on the two cell lines exhibited the same trends for syndecan-4 and caveolin-1 as was observed for endocytosis of the polyplexes. This study gives a clue how to take strategies by up- or down-regulation of the expressions of syndecan-4 and caveolin-1 to improve in vivo gene delivery efficiency of the PEG-PAMAM dendrimers in clinical transgenic therapy. (C) 2014 Elsevier B.V. All rights reserved.

语种英语
所属项目编号30971241 ; 81270368 ; RO1-EB005028
资助者National Natural Science Foundation of China ; federal funds from the National Institute of Biomedical Imaging and Bioengineering of the U.S.A. ; National Natural Science Foundation of China ; federal funds from the National Institute of Biomedical Imaging and Bioengineering of the U.S.A.
WOS记录号WOS:000346951200010
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53805
Collection北京大学基础医学院_心血管所
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
3.Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA
Recommended Citation
GB/T 7714
Shen, Wenwen,van Dongen, Mallory A.,Han, Yingchun,et al. The role of caveolin-1 and syndecan-4 in the internalization of PEGylated PAMAM dendrimer polyplexes into myoblast and hepatic cells[J]. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,2014,88(3):658-663.
APA Shen, Wenwen.,van Dongen, Mallory A..,Han, Yingchun.,Yu, Maomao.,Li, Yanzhi.,...&Qi, Rong.(2014).The role of caveolin-1 and syndecan-4 in the internalization of PEGylated PAMAM dendrimer polyplexes into myoblast and hepatic cells.EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS,88(3),658-663.
MLA Shen, Wenwen,et al."The role of caveolin-1 and syndecan-4 in the internalization of PEGylated PAMAM dendrimer polyplexes into myoblast and hepatic cells".EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS 88.3(2014):658-663.
Files in This Item: Download All
File Name/Size DocType Version Access License
1-s2.0-S093964111400(995KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Shen, Wenwen]'s Articles
[van Dongen, Mallory A.]'s Articles
[Han, Yingchun]'s Articles
百度学术
百度学术Similar articles in
[Shen, Wenwen]'s Articles
[van Dongen, Mallory A.]'s Articles
[Han, Yingchun]'s Articles
必应学术
必应学术Similar articles in
[Shen, Wenwen]'s Articles
[van Dongen, Mallory A.]'s Articles
[Han, Yingchun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 1-s2.0-S0939641114002355-main.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.