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学科主题: 临床医学
题名:
Enhanced angiogenesis and osteogenesis in critical bone defects by the controlled release of BMP-2 and VEGF: implantation of electron beam melting-fabricated porous Ti6Al4V scaffolds incorporating growth factor-doped fibrin glue
作者: Lv, Jia1; Xiu, Peng1; Tan, Jie1; Jia, Zhaojun2; Cai, Hong1; Liu, Zhongjun1
关键词: porous titanium ; electron beam melting (EBM) ; fibrin glue ; BMP-2 ; VEGF ; angiogenesis ; osteogenesis
刊名: BIOMEDICAL MATERIALS
发表日期: 2015-06-01
DOI: 10.1088/1748-6041/10/3/035013
卷: 10, 期:3
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: MORPHOGENETIC PROTEINS ; DUAL DELIVERY ; REGENERATION ; MODEL ; BIOMATERIALS ; EXPRESSION ; SEALANT ; BINDING ; SYSTEM ; REPAIR
英文摘要:

Electron beam melting (EBM)-fabricated porous titanium implants possessing low elastic moduli and tailored structures are promising biomaterials for orthopedic applications. However, the bio-inert nature of porous titanium makes reinforcement with growth factors (GFs) a promising method to enhance implant in vivo performance. Bone-morphogenic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) are key factors of angiogenesis and osteogenesis. Therefore, the present study is aimed at evaluating EBM-fabricated porous titanium implants incorporating GF-doped fibrin glue (FG) as composite scaffolds providing GFs for improvement of angiogenesis and osteogenesis in rabbit femoral condyle defects. BMP-2 and VEGF were added into the constituent compounds of FG, and then this GF-doped FG was subsequently injected into the porous scaffolds. In five groups of implants, angiogenesis and osteogenesis were evaluated at 4 weeks post-implantation using Microfil perfusion and histological analysis: eTi (empty scaffolds), cTi (containing undoped FG), BMP/cTi (containing 50 mu g rhBMP-2), VEGF/cTi (containing 0.5 mu g VEGF) and Dual/cTi (containing 50 mu g rhBMP-2 and 0.5 mu g VEGF). The results demonstrate that these composite implants are biocompatible and provide the desired gradual release of the bioactive growth factors. Incorporation of GF delivery, whether a single factor or dual factors, significantly enhanced both angiogenesis and osteogenesis inside the porous scaffolds. However, the synergistic effect of the dual factors combination was observable on angiogenesis but absent on osteogenesis. In conclusion, fibrin glue is a biocompatible material that could be employed as a delivery vehicle in EBM-fabricated porous titanium for controlled release of BMP-2 and VEGF. Application of this method for loading a porous titanium scaffold to incorporate growth factors is a convenient and promising strategy for improving osteogenesis of critical-sized bone defects.

语种: 英语
所属项目编号: Z121100005312005 ; Z141100002814008
项目资助者: Beijing AKEC Medical Co., Ltd ; Beijing Key Laboratory of Spinal Diseases of Peking University Third Hospital ; Medical Research Center of Peking University Third Hospital ; Project of Scientific and Technical Plan of Beijing
WOS记录号: WOS:000357093000027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53831
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100191, Peoples R China
2.Peking Univ, Ctr Biomed Mat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China

Recommended Citation:
Lv, Jia,Xiu, Peng,Tan, Jie,et al. Enhanced angiogenesis and osteogenesis in critical bone defects by the controlled release of BMP-2 and VEGF: implantation of electron beam melting-fabricated porous Ti6Al4V scaffolds incorporating growth factor-doped fibrin glue[J]. BIOMEDICAL MATERIALS,2015,10(3).
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