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Enhanced angiogenesis and osteogenesis in critical bone defects by the controlled release of BMP-2 and VEGF: implantation of electron beam melting-fabricated porous Ti6Al4V scaffolds incorporating growth factor-doped fibrin glue
Lv, Jia1; Xiu, Peng1; Tan, Jie1; Jia, Zhaojun2; Cai, Hong1; Liu, Zhongjun1
关键词Porous Titanium Electron Beam Melting (Ebm) Fibrin Glue Bmp-2 Vegf Angiogenesis Osteogenesis
刊名BIOMEDICAL MATERIALS
2015-06-01
DOI10.1088/1748-6041/10/3/035013
10期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]MORPHOGENETIC PROTEINS ; DUAL DELIVERY ; REGENERATION ; MODEL ; BIOMATERIALS ; EXPRESSION ; SEALANT ; BINDING ; SYSTEM ; REPAIR
英文摘要

Electron beam melting (EBM)-fabricated porous titanium implants possessing low elastic moduli and tailored structures are promising biomaterials for orthopedic applications. However, the bio-inert nature of porous titanium makes reinforcement with growth factors (GFs) a promising method to enhance implant in vivo performance. Bone-morphogenic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) are key factors of angiogenesis and osteogenesis. Therefore, the present study is aimed at evaluating EBM-fabricated porous titanium implants incorporating GF-doped fibrin glue (FG) as composite scaffolds providing GFs for improvement of angiogenesis and osteogenesis in rabbit femoral condyle defects. BMP-2 and VEGF were added into the constituent compounds of FG, and then this GF-doped FG was subsequently injected into the porous scaffolds. In five groups of implants, angiogenesis and osteogenesis were evaluated at 4 weeks post-implantation using Microfil perfusion and histological analysis: eTi (empty scaffolds), cTi (containing undoped FG), BMP/cTi (containing 50 mu g rhBMP-2), VEGF/cTi (containing 0.5 mu g VEGF) and Dual/cTi (containing 50 mu g rhBMP-2 and 0.5 mu g VEGF). The results demonstrate that these composite implants are biocompatible and provide the desired gradual release of the bioactive growth factors. Incorporation of GF delivery, whether a single factor or dual factors, significantly enhanced both angiogenesis and osteogenesis inside the porous scaffolds. However, the synergistic effect of the dual factors combination was observable on angiogenesis but absent on osteogenesis. In conclusion, fibrin glue is a biocompatible material that could be employed as a delivery vehicle in EBM-fabricated porous titanium for controlled release of BMP-2 and VEGF. Application of this method for loading a porous titanium scaffold to incorporate growth factors is a convenient and promising strategy for improving osteogenesis of critical-sized bone defects.

语种英语
WOS记录号WOS:000357093000027
项目编号Z121100005312005 ; Z141100002814008
资助机构Beijing AKEC Medical Co., Ltd ; Beijing Key Laboratory of Spinal Diseases of Peking University Third Hospital ; Medical Research Center of Peking University Third Hospital ; Project of Scientific and Technical Plan of Beijing
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53831
专题北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100191, Peoples R China
2.Peking Univ, Ctr Biomed Mat & Tissue Engn, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Lv, Jia,Xiu, Peng,Tan, Jie,et al. Enhanced angiogenesis and osteogenesis in critical bone defects by the controlled release of BMP-2 and VEGF: implantation of electron beam melting-fabricated porous Ti6Al4V scaffolds incorporating growth factor-doped fibrin glue[J]. BIOMEDICAL MATERIALS,2015,10(3).
APA Lv, Jia,Xiu, Peng,Tan, Jie,Jia, Zhaojun,Cai, Hong,&Liu, Zhongjun.(2015).Enhanced angiogenesis and osteogenesis in critical bone defects by the controlled release of BMP-2 and VEGF: implantation of electron beam melting-fabricated porous Ti6Al4V scaffolds incorporating growth factor-doped fibrin glue.BIOMEDICAL MATERIALS,10(3).
MLA Lv, Jia,et al."Enhanced angiogenesis and osteogenesis in critical bone defects by the controlled release of BMP-2 and VEGF: implantation of electron beam melting-fabricated porous Ti6Al4V scaffolds incorporating growth factor-doped fibrin glue".BIOMEDICAL MATERIALS 10.3(2015).
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