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学科主题: 精神卫生
题名:
Tenuifolin, an extract derived from tenuigenin, inhibits amyloid-beta secretion in vitro
作者: Lv, J.1; Jia, H.2; Jiang, Y.3; Ruan, Y.1; Liu, Z.1; Yue, W.1; Beyreuther, K.4; Tu, P.3; Zhang, D.1
关键词: Alzheimer&prime ; s disease ; amyloid beta-protein ; amyloid precursor protein ; tenuifolin ; tenuigenin
刊名: ACTA PHYSIOLOGICA
发表日期: 2009-08-01
DOI: 10.1111/j.1748-1716.2009.01961.x
卷: 196, 期:4, 页:419-425
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Physiology
研究领域[WOS]: Physiology
关键词[WOS]: ALZHEIMERS-DISEASE ; POLYGALA-TENUIFOLIA ; A-BETA ; PROTEIN ; BACE1 ; GENERATION ; SITE ; PEPTIDE ; NEURONS ; CELLS
英文摘要:

Aim: Previous studies have shown that tenuigenin, a crude extract of Polygala tenuifolia Willd. that is commonly used in traditional Chinese herbal medicine for memory loss, can reduce the secretion of Ab from cultured cells. However, the mechanism underlying this effect and the active compound derived from tenuigenin is unknown. In this study, a purified component of tenuigenin, tenuifolin, was examined and revealed to be an effective compound in vitro.

Methods: A beta secretion from three sets of COS-7 cells, each carrying a plasmid expressing a different form of APP was examined following the treatment with tenuifolin. Initially, tenuifolin was determined to have no inherent toxicity to either the transfected or wild type cells at the effective concentrations. Cells were then treated with 0.5-2.0 mu g mL(-1) tenuifolin for 12 h and their media were examined via an ELISA for A beta 1-40 and A beta-42.

Results: We found that treatment with 2.0 mu g mL(-1) tenuifolin significantly decreased Ab secretion from COS-7 cells without altering the ratio of A beta 1-40 and A beta-42. This effect is most probably due to inhibition of the beta-site APP cleaving enzyme as A beta secretion was not inhibited from cells expressing the C99 fragment.

Conclusion: Tenuifolin is an effective compound from tenuigenin. We believe that this finding should lead the way for future experiments to determine the exact mechanism for tenuifolin′s effect on A beta secretion.

语种: 英语
所属项目编号: 39970930 ; G1999064007
项目资助者: National Natural Sciences Foundation of China ; Major State Basic Research Development program ; People&prime ; s Republic of China
WOS记录号: WOS:000267948300007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53838
Appears in Collections:北京大学精神卫生研究所_期刊论文

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作者单位: 1.Peking Univ, Inst Mental Hlth, Beijing 100083, Peoples R China
2.Capital Univ, Beijing Anding Hosp Affiliated, Beijing, Peoples R China
3.Peking Univ, Coll Pharm, Beijing 100083, Peoples R China
4.Univ Heidelberg, Ctr Mol Biol, D-6900 Heidelberg, Germany

Recommended Citation:
Lv, J.,Jia, H.,Jiang, Y.,et al. Tenuifolin, an extract derived from tenuigenin, inhibits amyloid-beta secretion in vitro[J]. ACTA PHYSIOLOGICA,2009,196(4):419-425.
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