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Tenuifolin, an extract derived from tenuigenin, inhibits amyloid-beta secretion in vitro
Lv, J.1; Jia, H.2; Jiang, Y.3; Ruan, Y.1; Liu, Z.1; Yue, W.1; Beyreuther, K.4; Tu, P.3; Zhang, D.1
关键词Alzheimer&Prime s Disease Amyloid Beta-protein Amyloid Precursor Protein Tenuifolin Tenuigenin
刊名ACTA PHYSIOLOGICA
2009-08-01
DOI10.1111/j.1748-1716.2009.01961.x
196期:4页:419-425
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Physiology
研究领域[WOS]Physiology
关键词[WOS]ALZHEIMERS-DISEASE ; POLYGALA-TENUIFOLIA ; A-BETA ; PROTEIN ; BACE1 ; GENERATION ; SITE ; PEPTIDE ; NEURONS ; CELLS
英文摘要

Aim: Previous studies have shown that tenuigenin, a crude extract of Polygala tenuifolia Willd. that is commonly used in traditional Chinese herbal medicine for memory loss, can reduce the secretion of Ab from cultured cells. However, the mechanism underlying this effect and the active compound derived from tenuigenin is unknown. In this study, a purified component of tenuigenin, tenuifolin, was examined and revealed to be an effective compound in vitro.

Methods: A beta secretion from three sets of COS-7 cells, each carrying a plasmid expressing a different form of APP was examined following the treatment with tenuifolin. Initially, tenuifolin was determined to have no inherent toxicity to either the transfected or wild type cells at the effective concentrations. Cells were then treated with 0.5-2.0 mu g mL(-1) tenuifolin for 12 h and their media were examined via an ELISA for A beta 1-40 and A beta-42.

Results: We found that treatment with 2.0 mu g mL(-1) tenuifolin significantly decreased Ab secretion from COS-7 cells without altering the ratio of A beta 1-40 and A beta-42. This effect is most probably due to inhibition of the beta-site APP cleaving enzyme as A beta secretion was not inhibited from cells expressing the C99 fragment.

Conclusion: Tenuifolin is an effective compound from tenuigenin. We believe that this finding should lead the way for future experiments to determine the exact mechanism for tenuifolin′s effect on A beta secretion.

语种英语
WOS记录号WOS:000267948300007
项目编号39970930 ; G1999064007
资助机构National Natural Sciences Foundation of China ; Major State Basic Research Development program ; People&prime ; s Republic of China
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53838
专题北京大学精神卫生研究所
北京大学第一临床医学院_妇产科
北京大学第一临床医学院_皮肤性病科
作者单位1.Peking Univ, Inst Mental Hlth, Beijing 100083, Peoples R China
2.Capital Univ, Beijing Anding Hosp Affiliated, Beijing, Peoples R China
3.Peking Univ, Coll Pharm, Beijing 100083, Peoples R China
4.Univ Heidelberg, Ctr Mol Biol, D-6900 Heidelberg, Germany
推荐引用方式
GB/T 7714
Lv, J.,Jia, H.,Jiang, Y.,et al. Tenuifolin, an extract derived from tenuigenin, inhibits amyloid-beta secretion in vitro[J]. ACTA PHYSIOLOGICA,2009,196(4):419-425.
APA Lv, J..,Jia, H..,Jiang, Y..,Ruan, Y..,Liu, Z..,...&Zhang, D..(2009).Tenuifolin, an extract derived from tenuigenin, inhibits amyloid-beta secretion in vitro.ACTA PHYSIOLOGICA,196(4),419-425.
MLA Lv, J.,et al."Tenuifolin, an extract derived from tenuigenin, inhibits amyloid-beta secretion in vitro".ACTA PHYSIOLOGICA 196.4(2009):419-425.
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