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Human Epididymis Protein 4 Inhibits Proliferation of Human Ovarian Cancer Cells Via the Mitogen-Activated Protein Kinase and Phosphoinositide 3-Kinase/AKT Pathways
Kong, Xiuli; Chang, Xiaohong; Cheng, Hongyan; Ma, RuiQiong; Ye, Xue; Cui, Heng
关键词Epithelial Ovarian Cancer Human Epididymis Protein 4 Proliferation Apoptosis
刊名INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
2014-03-01
DOI10.1097/IGC.0000000000000078
24期:3页:427-436
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Obstetrics & Gynecology
研究领域[WOS]Oncology ; Obstetrics & Gynecology
关键词[WOS]ENDOMETRIOTIC CYSTS ; 4-DISULFIDE CORE ; CYTOCHROME-C ; SERUM HE-4 ; EXPRESSION ; APOPTOSIS ; GROWTH ; CARCINOMA ; BIOMARKER ; GENES
英文摘要

Objectives Human epididymis protein 4 (HE4) is a promising novel biomarker for the detection of epithelial ovarian cancer (EOC). The role of HE4 in EOC tumorigenesis is unclear. This study investigated the cellular and molecular mechanisms of HE4 in ovarian cancer cell proliferation.

Methods We generated HE4-overexpressing SKOV3 cells and silenced HE4 gene expression in SKOV3.ip1 cells. We used the cell counting kit 8 assay to evaluate cell proliferation and Western blotting to analyze the expression of proliferation- and apoptosis-associated proteins such as Bax, Bcl-2, and caspase 3.

Results Overexpression of HE4 in SKOV3, an ovarian carcinoma cell line, inhibited cell proliferation, In contrast, HE4 silencing in SKOV3.ip1 cells promoted cell proliferation; however, conditioned medium containing HE4 and human recombinant HE4 protein had no effect on proliferation in both SKOV3 and SKOV3.ip1 cells. Human epididymis protein 4 inhibited MEK, extracellular signal-regulating kinase 1/2, and AKT phosphorylation but promoted c-Jun N-terminal protein kinase 1/2/3 and c-JUN phosphorylation; however, p38 phosphorylation was impaired in HE4-overexpressing and silenced cells. Human epididymis protein 4 had no effect on epidermal growth factor receptor phosphorylation or on the apoptosis-associated proteins Bax, Bcl-2, and caspase 3.

Conclusions Human epididymis protein 4 might play a protective role in the progression of EOC by inhibiting cell proliferation. Antiproliferative activity was mediated by intracellular HE4 and not the secreted protein. Human epididymis protein 4 might inhibit cell proliferation by regulating the mitogen-activated protein kinase and phosphoinositide 3-kinase/AKT signal transduction pathways in vitro.

语种英语
WOS记录号WOS:000332519000009
项目编号81172454 ; 20100001110079
资助机构National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53842
专题北京大学第二临床医学院_妇科肿瘤中心
作者单位Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Kong, Xiuli,Chang, Xiaohong,Cheng, Hongyan,et al. Human Epididymis Protein 4 Inhibits Proliferation of Human Ovarian Cancer Cells Via the Mitogen-Activated Protein Kinase and Phosphoinositide 3-Kinase/AKT Pathways[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2014,24(3):427-436.
APA Kong, Xiuli,Chang, Xiaohong,Cheng, Hongyan,Ma, RuiQiong,Ye, Xue,&Cui, Heng.(2014).Human Epididymis Protein 4 Inhibits Proliferation of Human Ovarian Cancer Cells Via the Mitogen-Activated Protein Kinase and Phosphoinositide 3-Kinase/AKT Pathways.INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,24(3),427-436.
MLA Kong, Xiuli,et al."Human Epididymis Protein 4 Inhibits Proliferation of Human Ovarian Cancer Cells Via the Mitogen-Activated Protein Kinase and Phosphoinositide 3-Kinase/AKT Pathways".INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 24.3(2014):427-436.
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