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学科主题: 基础医学
题名:
Decorin-induced proliferation of avian myoblasts involves the myostatin/Smad signaling pathway
作者: Zeng, Q. J.1; Wang, L. N.1; Shu, G.1; Wang, S. B.1; Zhu, X. T.1; Gao, P.1; Xi, Q. Y.1; Zhang, Y. L.1; Zhang, Z. Q.2; Jiang, Q. Y.1
关键词: QM7 myoblast ; decorin ; myostatin ; cell proliferation ; Smad3
刊名: POULTRY SCIENCE
发表日期: 2014
DOI: 10.3382/ps.2013-03300
卷: 93, 期:1, 页:138-146
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Agriculture, Dairy & Animal Science
研究领域[WOS]: Agriculture
关键词[WOS]: GROWTH-FACTOR-BETA ; IN-VIVO ; DEVELOPMENTAL EXPRESSION ; TRANSCRIPTION FACTORS ; CONNECTIVE-TISSUE ; MUSCLE GROWTH ; DIFFERENTIATION ; MECHANISM ; MYOGENESIS ; CELLS
英文摘要:

Decorin, a small leucine-rich proteoglycan as a component of the extracellular matrix, plays an important role in the skeletal muscle development. It has been reported that decorin promoted proliferation and differentiation of muscle cells by restraining myostatin activity in rodents. However, the effects and mechanisms of decorin on avian myoblast proliferation are not understood clearly. Thus, in our research, decorin overexpressing and knocking-down quail myoblast-7 (QM7) myoblasts were established to explore the effects of decorin on avian myoblast proliferation by flow cytometry. The results showed that overexpression of decorin enhanced the proliferation of QM7 myoblasts, which was accompanied by the upregulation of follistatin and primary muscle regulatory factors (i.e., myogenic factor 5, myogenic factor 1, myogenin), and downregulation of myostatin expression, as well as the decreased phosphorylation level of SMAD family member 3 (Smad3). In line with expectations, decorin RNAi displayed an opposite effect on the proliferation and gene expression pattern of QM7 cells. In conclusion, our in vitro studies suggested the decorin-mediated myostatin/Smad signaling pathway might be involved in the regulation of avian myoblast proliferation.

语种: 英语
所属项目编号: 31101780 ; 2009CB941601 ; 20114404120001
项目资助者: National Natural Science Foundation for the Young Scholars of China (Beijing) ; National Basic Research Program of China-the 973 Program (Beijing) ; Research Fund for the Doctoral Program of Higher Education (Beijing)
WOS记录号: WOS:000334041800019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53892
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.South China Agr Univ, Coll Anim Sci, ALLTECH SCAU Anim Nutr Control Res Alliance, Guangzhou 510642, Guangdong, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Dept Cell Biol, Minist Educ,Key Lab Carcinogenesis & Translat Res, Beijing 100142, Peoples R China

Recommended Citation:
Zeng, Q. J.,Wang, L. N.,Shu, G.,et al. Decorin-induced proliferation of avian myoblasts involves the myostatin/Smad signaling pathway[J]. POULTRY SCIENCE,2014,93(1):138-146.
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