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学科主题基础医学
5-Azacytidine induces early stage apoptosis and promotes in vitro maturation by changing chromosomal construction in murine oocytes
Zhao, F. Y.1; Shao, C. P.2; Li, Y.2; Ma, W. Y.1; Tian, N.1; Zheng, J. H.1
关键词Oocytes Mouse 5-azacytidine Decondensation Early Apoptosis Maturation In Vitro Hypomethylation
刊名REPRODUCTIVE TOXICOLOGY
2013-06-01
DOI10.1016/j.reprotox.2013.01.007
37页:56-61
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Reproductive Biology ; Toxicology
研究领域[WOS]Reproductive Biology ; Toxicology
关键词[WOS]GROWTH-DIFFERENTIATION FACTOR-9 ; BONE MORPHOGENETIC PROTEIN-15 ; BETA SUPERFAMILY MEMBERS ; DNA METHYLATION ; CELL-DEATH ; TGF-BETA ; OVARIAN-FUNCTION ; BOVINE OOCYTES ; STEM-CELLS ; CANCER
英文摘要

As an anticancer drug, 5-azacytidine (5-AzaC) has been widely used to treat various cancers. To investigate the effect of 5-AzaC on mouse oocytes cultured in vitro, we have performed morphological and molecular biology studies to examine the behavior of chromosomes and oocyte development. In 5-AzaC-treated oocytes, chromosomes were decondensed and unstable. The mRNA levels of Caspase3, Caspase8, and Caspase9 increased with the occurrence of early stage apoptosis in oocytes following 5-AzaC treatment. Furthermore, the mRNA levels of Gdf9 and Bmp15 also increased with the corresponding morphological changes in 5-AzaC-treated oocytes. In conclusion, 5-AzaC not only induced early apoptosis through both extrinsic and intrinsic pathways, but also had a positive effect on the developmental competence of mouse oocytes during in vitro maturation. These effects may be due to changes in chromosomal construction induced by DNA hypomethylation. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000317254100008
项目编号90919012 ; 10874099 ; 2007CB5119004 ; 20090002110065 ; 2010THZ01
资助机构National Nature Science Foundation of China ; Chinese National Basic Research Program ; Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53896
专题北京大学基础医学院
作者单位1.Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Histol & Embryol, Beijing 100191, Peoples R China
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GB/T 7714
Zhao, F. Y.,Shao, C. P.,Li, Y.,et al. 5-Azacytidine induces early stage apoptosis and promotes in vitro maturation by changing chromosomal construction in murine oocytes[J]. REPRODUCTIVE TOXICOLOGY,2013,37:56-61.
APA Zhao, F. Y.,Shao, C. P.,Li, Y.,Ma, W. Y.,Tian, N.,&Zheng, J. H..(2013).5-Azacytidine induces early stage apoptosis and promotes in vitro maturation by changing chromosomal construction in murine oocytes.REPRODUCTIVE TOXICOLOGY,37,56-61.
MLA Zhao, F. Y.,et al."5-Azacytidine induces early stage apoptosis and promotes in vitro maturation by changing chromosomal construction in murine oocytes".REPRODUCTIVE TOXICOLOGY 37(2013):56-61.
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