北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
5-Azacytidine induces early stage apoptosis and promotes in vitro maturation by changing chromosomal construction in murine oocytes
作者: Zhao, F. Y.1; Shao, C. P.2; Li, Y.2; Ma, W. Y.1; Tian, N.1; Zheng, J. H.1
关键词: Oocytes ; Mouse ; 5-Azacytidine ; Decondensation ; Early apoptosis ; Maturation ; In vitro ; Hypomethylation
刊名: REPRODUCTIVE TOXICOLOGY
发表日期: 2013-06-01
DOI: 10.1016/j.reprotox.2013.01.007
卷: 37, 页:56-61
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Reproductive Biology ; Toxicology
研究领域[WOS]: Reproductive Biology ; Toxicology
关键词[WOS]: GROWTH-DIFFERENTIATION FACTOR-9 ; BONE MORPHOGENETIC PROTEIN-15 ; BETA SUPERFAMILY MEMBERS ; DNA METHYLATION ; CELL-DEATH ; TGF-BETA ; OVARIAN-FUNCTION ; BOVINE OOCYTES ; STEM-CELLS ; CANCER
英文摘要:

As an anticancer drug, 5-azacytidine (5-AzaC) has been widely used to treat various cancers. To investigate the effect of 5-AzaC on mouse oocytes cultured in vitro, we have performed morphological and molecular biology studies to examine the behavior of chromosomes and oocyte development. In 5-AzaC-treated oocytes, chromosomes were decondensed and unstable. The mRNA levels of Caspase3, Caspase8, and Caspase9 increased with the occurrence of early stage apoptosis in oocytes following 5-AzaC treatment. Furthermore, the mRNA levels of Gdf9 and Bmp15 also increased with the corresponding morphological changes in 5-AzaC-treated oocytes. In conclusion, 5-AzaC not only induced early apoptosis through both extrinsic and intrinsic pathways, but also had a positive effect on the developmental competence of mouse oocytes during in vitro maturation. These effects may be due to changes in chromosomal construction induced by DNA hypomethylation. (C) 2013 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 90919012 ; 10874099 ; 2007CB5119004 ; 20090002110065 ; 2010THZ01
项目资助者: National Nature Science Foundation of China ; Chinese National Basic Research Program ; Chinese Ministry of Education ; Tsinghua University Initiative Scientific Research Program
WOS记录号: WOS:000317254100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53896
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Tsinghua Univ, Dept Phys, State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Histol & Embryol, Beijing 100191, Peoples R China

Recommended Citation:
Zhao, F. Y.,Shao, C. P.,Li, Y.,et al. 5-Azacytidine induces early stage apoptosis and promotes in vitro maturation by changing chromosomal construction in murine oocytes[J]. REPRODUCTIVE TOXICOLOGY,2013,37:56-61.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, F. Y.]'s Articles
[Shao, C. P.]'s Articles
[Li, Y.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, F. Y.]‘s Articles
[Shao, C. P.]‘s Articles
[Li, Y.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace