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Calvarial defect healing by recruitment of autogenous osteogenic stem cells using locally applied simvastatin
Cui Yueyi1; Han Xiaoguang1; Wang Jingying1; Song Quansheng1; Tan Jie2; Fu Xin2; Xu Yingsheng3; Song Chunli1,2
关键词Simvastatin Bone Healing Mesenchymal Stem Cell Endothelial Progenitor Cells Bmp-2 Hif-1 Alpha
刊名BIOMATERIALS
2013-12-01
DOI10.1016/j.biomaterials.2013.08.060
34期:37页:9373-9380
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
资助者National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]ENDOTHELIAL PROGENITOR CELLS ; MARROW STROMAL CELLS ; SEGMENTAL BONE DEFECT ; MICE CEREBRAL INFARCT ; FUNCTIONAL RECOVERY ; GELATIN-HYDROGEL ; REPAIR ; MOBILIZATION ; RATS ; THERAPY
英文摘要

Local statins implant has been shown to promote bone healing, the underlying mechanisms are unclear. The purpose of this study was to test the effect of local simvastatin implant on bone defect healing; to evaluate the mobilization, migration, and homing of bone marrow-derived mesenchymal stem cells (BMSCs) and endothelial progenitor cells (EPCs) induced by simvastatin. We found that local simvastatin implant increased bone formation by 51.8% (week 6) and 64.8% (week 12) compared with polyglycolic acid controls (P < 0.01), as verified by X-ray, CT, and histology. Simvastatin increased migration capacity of BMSCs and EPCs in vitro (P < 0.05). Local simvastatin implant increased mobilization of EPCs to the peripheral blood by 127% revealed by FACS analysis (P < 0.01), and increased osteogenic BMSCs to the peripheral blood dramatically revealed by Alizarin Red-S staining for mineralized nodules formation. Pre-transplanted GFP-transfected BMSCs as a tracing cell and bioluminescence imaging revealed that local simvastatin implant recruited GFP-labeled BMSC. Also, local simvastatin implant induced the HIF-1 alpha and BMP-2 expression. In conclusion, local simvastatin implantation promotes bone defect healing, where the underlying mechanism appears to involve the higher expression of HIF-1 alpha and BMP-2, thus recruit autogenous osteogenic and angiogenetic stem cells to the bone defect area implanted with simvastatin. (C) 2013 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号81171693 ; 81100895 ; NCET-10-0202
资助者National Natural Science Foundation of China ; Program for New Century Excellent Talents in University ; National Natural Science Foundation of China ; Program for New Century Excellent Talents in University
WOS记录号WOS:000326901200014
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53900
专题北京大学第三临床医学院_骨科
作者单位1.Beijing Key Lab Spinal Dis, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Orthoped, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Neurol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Cui Yueyi,Han Xiaoguang,Wang Jingying,et al. Calvarial defect healing by recruitment of autogenous osteogenic stem cells using locally applied simvastatin[J]. BIOMATERIALS,2013,34(37):9373-9380.
APA Cui Yueyi.,Han Xiaoguang.,Wang Jingying.,Song Quansheng.,Tan Jie.,...&Song Chunli.(2013).Calvarial defect healing by recruitment of autogenous osteogenic stem cells using locally applied simvastatin.BIOMATERIALS,34(37),9373-9380.
MLA Cui Yueyi,et al."Calvarial defect healing by recruitment of autogenous osteogenic stem cells using locally applied simvastatin".BIOMATERIALS 34.37(2013):9373-9380.
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