IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Synthesizing oncogenic signal-processing systems that function as both "signal counters′′ and "signal blockers′′ in cancer cells
Liu, Yuchen1,2,3; Huang, Weiren1,3; Zhou, Dexi2; Han, Yonghua1,3; Duan, Yonggang1,3; Zhang, Xiaoyue1,2,3; Zhang, Hu2,3; Jiang, Zhimao3; Gui, Yaoting3; Cai, Zhiming1,3
刊名MOLECULAR BIOSYSTEMS
2013
DOI10.1039/c3mb70093c
9期:7页:1909-1918
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
资助者Basic Research Program of Shenzhen ; promotion Program for Shenzhen Key Laboratory ; National High Technology Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; PhD Programs Foundation of Ministry of Education of China ; Basic Research Program of Shenzhen ; promotion Program for Shenzhen Key Laboratory ; National High Technology Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; PhD Programs Foundation of Ministry of Education of China
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]FACTOR-KAPPA-B ; SYNTHETIC BIOLOGY ; GENE NETWORKS ; BETA-CATENIN ; TRANSLATION ; EXPRESSION ; PROTEIN ; QUADRUPLEXES ; INTEGRATOR ; ACTIVATION
英文摘要

RNA-protein interaction plays a significant role in regulating eukaryotic translation. This phenomenon raises questions about the ability of artificial biological systems to take the advantage of protein-RNA interaction. Here, we designed an oncogenic signal-processing system expressing both a Renilla luciferase reporter gene controlled by RNA-protein interaction in its 50-untranslated region (5′-UTR) and a Firefly luciferase normalization gene. To test the ability of the designed system, we then constructed vectors targeting the nuclear factor-kappa B (NF-kappa B) or the beta-catenin signal. We found that the inhibition (%) of luciferase expression was correlated to the targeted protein content, allowing quantitative measurement of oncogenic signal intensity in cancer cells. The systems inhibited the expression of oncogenic signal downstream genes and induced bladder cancer cell proliferation inhibition and apoptosis without affecting normal urothelial cells. Compared to traditional methods (ELISA and quantitative immunoblotting), the bio-systems provided highly accurate, consistent, and reproducible quantification of protein signals and were able to discriminate between cancerous and non-cancerous cells. In conclusion, the synthetic systems function as both "signal counters′′ and "signal blockers′′ in cancer cells. This approach provides a synthetic biology platform for oncogenic signal measurement and cancer treatment.

语种英语
所属项目编号JCYJ20120614155650545 ; ZDSY20120615154448514 ; 2012AA02A208 ; 81100460 ; 20100001110100
资助者Basic Research Program of Shenzhen ; promotion Program for Shenzhen Key Laboratory ; National High Technology Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; PhD Programs Foundation of Ministry of Education of China ; Basic Research Program of Shenzhen ; promotion Program for Shenzhen Key Laboratory ; National High Technology Research and Development Program of China (863 Program) ; National Natural Science Foundation of China ; PhD Programs Foundation of Ministry of Education of China
WOS记录号WOS:000319882200035
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53933
专题北京大学深圳医院
作者单位1.Anhui Med Univ, Hefei 230032, Peoples R China
2.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen 518039, Peoples R China
3.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen PKU HKUST Med Ctr,Inst Urol, Shenzhen 518036, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yuchen,Huang, Weiren,Zhou, Dexi,et al. Synthesizing oncogenic signal-processing systems that function as both "signal counters′′ and "signal blockers′′ in cancer cells[J]. MOLECULAR BIOSYSTEMS,2013,9(7):1909-1918.
APA Liu, Yuchen.,Huang, Weiren.,Zhou, Dexi.,Han, Yonghua.,Duan, Yonggang.,...&Cai, Zhiming.(2013).Synthesizing oncogenic signal-processing systems that function as both "signal counters′′ and "signal blockers′′ in cancer cells.MOLECULAR BIOSYSTEMS,9(7),1909-1918.
MLA Liu, Yuchen,et al."Synthesizing oncogenic signal-processing systems that function as both "signal counters′′ and "signal blockers′′ in cancer cells".MOLECULAR BIOSYSTEMS 9.7(2013):1909-1918.
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