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Liquid chromatography-tandem mass spectrometry analysis of protocatechuic aldehyde and its phase I and II metabolites in rat
Xu, Man; Zhang, Zichuan; Fu, Gang; Sun, Shifeng; Sun, Jianghao; Yang, Min; Liu, Aihua; Han, Jian; Guo, Dean
关键词Protocatechuic Aldehyde Metabolites Hplc-ms2 Salvia Miltiorrhiza
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2007-09-01
DOI10.1016/j.jchromb.2007.05.042
856期:1-2页:100-107
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]GLYCINE CONJUGATION ; SALVIA-MILTIORRHIZA ; PHENOLIC-ACIDS ; CAPILLARY-ELECTROPHORESIS ; URINARY-EXCRETION ; ROSMARINIC ACID ; CAFFEIC ACID ; PLASMA ; EXTRACT ; ROOTS
英文摘要

A method using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) analysis was established for the identification of metabolites in rat after oral administration of protocatechuic aldehyde, a major bioactive phenolic acid in the roots of Salvia miltiorrhiza. Eleven metabolites in rat plasma and urine were firstly identified as protocatechuic aldehyde, protocatechuic acid and their methylated, glucuronized or glycine conjugates on the basis of their MS fragmentation behaviors, while nine of these metabolites (except protocatechuic aldehyde and protocatechuic acid) were detected in rat bile. In addition, the possible metabolic pathway was proposed for the first time. In the phase I metabolism, protocatechuic aldehyde could be oxidized to protocatechuic acid. The conjugates would be formed in rat intestine, liver and kidney and excreted from rat urine and bile. Enthrohepatic circulation played an important role in the metabolism of protocatechuic aldehyde. The results proved that the established method was simple, reliable and sensitive, revealing that it could be used to rapid screen and identify the structures of active components responsible for pharmacological effects of protocatechuic aldehyde and to better understand its in vivo metabolism. (C) 2007 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000249615100016
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53936
专题北京大学药学院
作者单位Peking Univ, Sch Pharmaceut Sci, Ctr Hlth Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
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Xu, Man,Zhang, Zichuan,Fu, Gang,et al. Liquid chromatography-tandem mass spectrometry analysis of protocatechuic aldehyde and its phase I and II metabolites in rat[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,856(1-2):100-107.
APA Xu, Man.,Zhang, Zichuan.,Fu, Gang.,Sun, Shifeng.,Sun, Jianghao.,...&Guo, Dean.(2007).Liquid chromatography-tandem mass spectrometry analysis of protocatechuic aldehyde and its phase I and II metabolites in rat.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,856(1-2),100-107.
MLA Xu, Man,et al."Liquid chromatography-tandem mass spectrometry analysis of protocatechuic aldehyde and its phase I and II metabolites in rat".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 856.1-2(2007):100-107.
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