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学科主题: 药学
题名:
Liquid chromatography-tandem mass spectrometry analysis of protocatechuic aldehyde and its phase I and II metabolites in rat
作者: Xu, Man; Zhang, Zichuan; Fu, Gang; Sun, Shifeng; Sun, Jianghao; Yang, Min; Liu, Aihua; Han, Jian; Guo, Dean
关键词: protocatechuic aldehyde ; metabolites ; HPLC-MS2 ; Salvia miltiorrhiza
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2007-09-01
DOI: 10.1016/j.jchromb.2007.05.042
卷: 856, 期:1-2, 页:100-107
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: GLYCINE CONJUGATION ; SALVIA-MILTIORRHIZA ; PHENOLIC-ACIDS ; CAPILLARY-ELECTROPHORESIS ; URINARY-EXCRETION ; ROSMARINIC ACID ; CAFFEIC ACID ; PLASMA ; EXTRACT ; ROOTS
英文摘要:

A method using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) analysis was established for the identification of metabolites in rat after oral administration of protocatechuic aldehyde, a major bioactive phenolic acid in the roots of Salvia miltiorrhiza. Eleven metabolites in rat plasma and urine were firstly identified as protocatechuic aldehyde, protocatechuic acid and their methylated, glucuronized or glycine conjugates on the basis of their MS fragmentation behaviors, while nine of these metabolites (except protocatechuic aldehyde and protocatechuic acid) were detected in rat bile. In addition, the possible metabolic pathway was proposed for the first time. In the phase I metabolism, protocatechuic aldehyde could be oxidized to protocatechuic acid. The conjugates would be formed in rat intestine, liver and kidney and excreted from rat urine and bile. Enthrohepatic circulation played an important role in the metabolism of protocatechuic aldehyde. The results proved that the established method was simple, reliable and sensitive, revealing that it could be used to rapid screen and identify the structures of active components responsible for pharmacological effects of protocatechuic aldehyde and to better understand its in vivo metabolism. (C) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000249615100016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53936
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, Ctr Hlth Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Xu, Man,Zhang, Zichuan,Fu, Gang,et al. Liquid chromatography-tandem mass spectrometry analysis of protocatechuic aldehyde and its phase I and II metabolites in rat[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,856(1-2):100-107.
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