北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
Understanding three-dimensional spatial relationship between the mouse second polar body and first cleavage plane with full-field optical coherence tomography
作者: Zheng, Jing-gao1,2; Huo, Tiancheng1,2; Chen, Tianyuan1,2; Wang, Chengming1,2; Zhang, Ning1,2; Tian, Ning1,2; Zhao, Fengying1,2; Lu, Danyu3; Chen, Dieyan1,2; Ma, Wanyun1,2; Sun, Jia-lin1,2; Xue, Ping1,2
关键词: label-free imaging ; preimplantation mouse embryos ; full-field optical coherence tomography ; early patterning and polarity
刊名: JOURNAL OF BIOMEDICAL OPTICS
发表日期: 2013
DOI: 10.1117/1.JBO.18.1.010503
卷: 18, 期:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: EGG ; BLASTOMERES ; MICROSCOPY ; EMBRYO ; FATES
英文摘要:

The morphogenetic relationship between early patterning and polarity formation is of fundamental interest and remains a controversial issue in preimplantation embryonic development. We use a label-free three-dimensional (3-D) imaging technique of full-field optical coherence tomography (FF-OCT) successfully for the first time to study the dynamics of developmental processes in mouse preimplantation lives. Label-free 3-D subcellular time-lapse images are demonstrated to investigate 3-D spatial relationship between the second polar body (2PB) and the first cleavage plane. By using FF-OCT together with quantitative study, we show that only 25% of the predicted first cleavage planes, defined by the apposing plane of two pronuclei, pass through the 2PB. Also only 27% of the real cleavage planes pass through the 2PB. These results suggest that the 2PB is not a convincing spatial cue for the event of the first cleavage. Our studies demonstrate the feasibility of FF-OCT in providing new insights and potential breakthroughs to the controversial issues of early patterning and polarity in mammalian developmental biology. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.18.1.010503]

语种: 英语
所属项目编号: 2006AA02Z472 ; 2007CB51 1904 ; 001CB510307 ; 2009CB929400 ; 61227807 ; 2010THZ01
项目资助者: Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Tsinghua Initiative Scientific Research Program
WOS记录号: WOS:000315157000003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53940
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China
2.State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
3.Peking Univ, Dept Anat & Histol & Embryol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zheng, Jing-gao,Huo, Tiancheng,Chen, Tianyuan,et al. Understanding three-dimensional spatial relationship between the mouse second polar body and first cleavage plane with full-field optical coherence tomography[J]. JOURNAL OF BIOMEDICAL OPTICS,2013,18(1).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zheng, Jing-gao]'s Articles
[Huo, Tiancheng]'s Articles
[Chen, Tianyuan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zheng, Jing-gao]‘s Articles
[Huo, Tiancheng]‘s Articles
[Chen, Tianyuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace