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学科主题: 基础医学
题名:
Understanding three-dimensional spatial relationship between the mouse second polar body and first cleavage plane with full-field optical coherence tomography
作者: Zheng, Jing-gao1,2; Huo, Tiancheng1,2; Chen, Tianyuan1,2; Wang, Chengming1,2; Zhang, Ning1,2; Tian, Ning1,2; Zhao, Fengying1,2; Lu, Danyu3; Chen, Dieyan1,2; Ma, Wanyun1,2; Sun, Jia-lin1,2; Xue, Ping1,2
关键词: label-free imaging ; preimplantation mouse embryos ; full-field optical coherence tomography ; early patterning and polarity
刊名: JOURNAL OF BIOMEDICAL OPTICS
发表日期: 2013
DOI: 10.1117/1.JBO.18.1.010503
卷: 18, 期:1
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: EGG ; BLASTOMERES ; MICROSCOPY ; EMBRYO ; FATES
英文摘要:

The morphogenetic relationship between early patterning and polarity formation is of fundamental interest and remains a controversial issue in preimplantation embryonic development. We use a label-free three-dimensional (3-D) imaging technique of full-field optical coherence tomography (FF-OCT) successfully for the first time to study the dynamics of developmental processes in mouse preimplantation lives. Label-free 3-D subcellular time-lapse images are demonstrated to investigate 3-D spatial relationship between the second polar body (2PB) and the first cleavage plane. By using FF-OCT together with quantitative study, we show that only 25% of the predicted first cleavage planes, defined by the apposing plane of two pronuclei, pass through the 2PB. Also only 27% of the real cleavage planes pass through the 2PB. These results suggest that the 2PB is not a convincing spatial cue for the event of the first cleavage. Our studies demonstrate the feasibility of FF-OCT in providing new insights and potential breakthroughs to the controversial issues of early patterning and polarity in mammalian developmental biology. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.18.1.010503]

语种: 英语
所属项目编号: 2006AA02Z472 ; 2007CB51 1904 ; 001CB510307 ; 2009CB929400 ; 61227807 ; 2010THZ01
项目资助者: Ministry of Science and Technology of China ; National Natural Science Foundation of China ; Tsinghua Initiative Scientific Research Program
WOS记录号: WOS:000315157000003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53940
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Tsinghua Univ, Dept Phys, Beijing 100084, Peoples R China
2.State Key Lab Low Dimens Quantum Phys, Beijing 100084, Peoples R China
3.Peking Univ, Dept Anat & Histol & Embryol, Sch Basic Med Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zheng, Jing-gao,Huo, Tiancheng,Chen, Tianyuan,et al. Understanding three-dimensional spatial relationship between the mouse second polar body and first cleavage plane with full-field optical coherence tomography[J]. JOURNAL OF BIOMEDICAL OPTICS,2013,18(1).
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