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学科主题: 临床医学
题名:
Molecular Characterization of Pathogenic Members of the Genus Fonsecaea Using Multilocus Analysis
作者: Sun, Jiufeng1,2,3; Najafzadeh, Mohammed J.3,4,5,6; van den Ende, Albertus H. G. Gerrits3; Vicente, Vania A.7; Feng, Peiying3,8; Xi, Liyan1; De Hoog, Gerrit S.1,3,4,9
刊名: PLOS ONE
发表日期: 2012-08-02
DOI: 10.1371/journal.pone.0041512
卷: 7, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: MELANIN-LIKE PIGMENTS ; YEAST-LIKE FUNGI ; IN-VITRO ; INFECTION ; CHROMOBLASTOMYCOSIS ; DERMATITIDIS ; DIVERSITY ; LACCASE ; CDC42 ; POLYMORPHISM
英文摘要:

Members of the fungal genus Fonsecaea causing human chromoblastomycosis show substantial geographic structuring. Genetic identity of clinical and environmental strains suggests transmission from plant debris, while the evolutionary processes that have led to spatially separated populations have remained unexplained. Sequences of ITS, BT2, ACT1, Cdc42, Lac and HmgA were analyzed, either by direct sequencing or by cloning. Thirty-seven clinical and environmental Fonsecaea strains from Central and South America, Asia, Africa and Europe were sequenced and possible recombination events were calculated. Phylogenetic trees of Cdc42, Lac and HmgA were statistically supported, but ITS, BT2 and ACT1 trees were not. The Standardized Index of Association (I-A(S)) did not detect recombination (I-A(S) = 0.4778), neither did the Phi-test for separate genes. In Fonsecaea nubica non-synonymous mutations causing functional changes were observed in Lac gene, even though no selection pressures were detected with the neutrality test (Tajima D test, p>0.05). Genetic differentiation of populations for each gene showed separation of American, African and Asian populations. Strains of clinical vs. environmental origin showed genetic distances that were comparable or lower than found in geographic differentiation. In conclusion, here we demonstrated clonality of sibling species using multilocus data, geographic structuring of populations, and a low functional and structural selective constraint during evolution of the genus Fonsecaea.

语种: 英语
所属项目编号: U0932009 ; CEVF-00007
项目资助者: National Natural Science Foundation of China ; Federation of European Biochemical Societies (FEBS) Chinese European Visiting Fellowships ; International Program of Project 985 at Sun Yat-sen University
WOS记录号: WOS:000307184700015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53944
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
2.Mashhad Univ Med Sci, Dept Parasitol & Mycol, Mashhad, Iran
3.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Dermatol, Guangzhou 510275, Guangdong, Peoples R China
4.Sun Yat Sen Univ, Key Lab Trop Dis Control, Minist Educ, Dept Parasitol,Zhongshan Sch Med, Guangzhou 510275, Guangdong, Peoples R China
5.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, NL-1012 WX Amsterdam, Netherlands
6.Mashhad Univ Med Sci, Sch Med, Ghaem Hosp, Canc Mol Pathol Res Ctr, Mashhad, Iran
7.Univ Fed Parana, Dept Basic Pathol, BR-80060000 Curitiba, Parana, Brazil
8.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Dermatol, Guangzhou 510275, Guangdong, Peoples R China
9.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China

Recommended Citation:
Sun, Jiufeng,Najafzadeh, Mohammed J.,van den Ende, Albertus H. G. Gerrits,et al. Molecular Characterization of Pathogenic Members of the Genus Fonsecaea Using Multilocus Analysis[J]. PLOS ONE,2012,7(8).
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