IR@PKUHSC  > 北京大学临床肿瘤学院  > 肿瘤外科
学科主题临床医学
The extent of inflammatory infiltration in primary cancer tissues is associated with lymphomagenesis in immunodeficient mice
Zhang, Lianhai1; Liu, Yiqiang2; Wang, Xiaohong1; Tang, Zhiyu3; Li, Shuangxi1; Hu, Ying1; Zong, Xianglong1; Wu, Xiaojiang1; Bu, Zhaode1; Wu, Aiwen1; Li, Ziyu1; Li, Zhongwu2; Huang, Xiaozheng2; Jia, Ling2; Kang, Qiang2; Liu, Yong3; Sutton, David3; Wang, Lai3; Luo, Lusong3; Ji, Jiafu1
刊名SCIENTIFIC REPORTS
2015-03-30
DOI10.1038/srep09447
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]EPSTEIN-BARR-VIRUS ; CELL LUNG-CANCER ; XENOGRAFTS ; MODELS ; ESTABLISHMENT
英文摘要

Xenotransplantation of human cancers into immunodeficient mice is a very useful approach for studying human tumor biology. However, the occasional occurrence of lymphomagenesis in some mice can spoil the model and must be investigated in detail. We found that a high percentage (32.5%, 26/80) of cancer patient-derived xenografts (PDXs) resembled lymphoma in NOD/SCID mice. Of the 26 xenografts, 23 were human-derived expressing human CD45 (hCD45+) and proved to be of the B-cell subtype (CD3-/CD20+), and they were all positive for Epstein - Barr virus (EBV). The remaining 3 xenografts proved to be mouse-derived for both hCD45-and negative amplification of a human gene. The most interesting finding is that gastric cancer had much higher rates (24/126, 19.0%) of lymphoma formation in the PDX model than did colorectal cancer (1/43, 2.3%). Statistical analysis revealed that cancer type and inflammation in the parent tumor are significantly associated with lymphomagenesis. Further validation discovered lymphomagenesis by inoculating only gastritis mucosa. Therefore, our findings suggest that it is necessary to take precautions when directly xenografting cancer tissues with remarkable baseline inflammation, such as gastric cancer into immunodeficient NOD/SCID strains. Further, the established xenograft models should be validated by both leukocyte markers and human gene signatures.

语种英语
WOS记录号WOS:000351938100001
项目编号2012AA02A203-B01 ; 2012AA02A504-B01 ; 2012AA020101 ; Z121100007512010 ; D131100005313010
资助机构National Key Technology RD Program ; Beijing Municipal Science &amp ; Technology Commission
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53947
专题北京大学临床肿瘤学院_肿瘤外科
北京大学临床肿瘤学院_胃肠肿瘤中心一病区
北京大学临床肿瘤学院_病理科
作者单位1.Peking Univ, Canc Hosp & Inst, Dept Surg, Key Lab Carcinogenesis & Translat Res,Minist Edu, Beijing 100871, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Dept Pathol, Beijing 100871, Peoples R China
3.BeiGene Beijing Co Ltd, Life Sci Pk, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Lianhai,Liu, Yiqiang,Wang, Xiaohong,et al. The extent of inflammatory infiltration in primary cancer tissues is associated with lymphomagenesis in immunodeficient mice[J]. SCIENTIFIC REPORTS,2015,5.
APA Zhang, Lianhai.,Liu, Yiqiang.,Wang, Xiaohong.,Tang, Zhiyu.,Li, Shuangxi.,...&Ji, Jiafu.(2015).The extent of inflammatory infiltration in primary cancer tissues is associated with lymphomagenesis in immunodeficient mice.SCIENTIFIC REPORTS,5.
MLA Zhang, Lianhai,et al."The extent of inflammatory infiltration in primary cancer tissues is associated with lymphomagenesis in immunodeficient mice".SCIENTIFIC REPORTS 5(2015).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Lianhai]的文章
[Liu, Yiqiang]的文章
[Wang, Xiaohong]的文章
百度学术
百度学术中相似的文章
[Zhang, Lianhai]的文章
[Liu, Yiqiang]的文章
[Wang, Xiaohong]的文章
必应学术
必应学术中相似的文章
[Zhang, Lianhai]的文章
[Liu, Yiqiang]的文章
[Wang, Xiaohong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。