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学科主题: 临床医学
题名:
The extent of inflammatory infiltration in primary cancer tissues is associated with lymphomagenesis in immunodeficient mice
作者: Zhang, Lianhai1; Liu, Yiqiang2; Wang, Xiaohong1; Tang, Zhiyu3; Li, Shuangxi1; Hu, Ying1; Zong, Xianglong1; Wu, Xiaojiang1; Bu, Zhaode1; Wu, Aiwen1; Li, Ziyu1; Li, Zhongwu2; Huang, Xiaozheng2; Jia, Ling2; Kang, Qiang2; Liu, Yong3; Sutton, David3; Wang, Lai3; Luo, Lusong3; Ji, Jiafu1
刊名: SCIENTIFIC REPORTS
发表日期: 2015-03-30
DOI: 10.1038/srep09447
卷: 5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: EPSTEIN-BARR-VIRUS ; CELL LUNG-CANCER ; XENOGRAFTS ; MODELS ; ESTABLISHMENT
英文摘要:

Xenotransplantation of human cancers into immunodeficient mice is a very useful approach for studying human tumor biology. However, the occasional occurrence of lymphomagenesis in some mice can spoil the model and must be investigated in detail. We found that a high percentage (32.5%, 26/80) of cancer patient-derived xenografts (PDXs) resembled lymphoma in NOD/SCID mice. Of the 26 xenografts, 23 were human-derived expressing human CD45 (hCD45+) and proved to be of the B-cell subtype (CD3-/CD20+), and they were all positive for Epstein - Barr virus (EBV). The remaining 3 xenografts proved to be mouse-derived for both hCD45-and negative amplification of a human gene. The most interesting finding is that gastric cancer had much higher rates (24/126, 19.0%) of lymphoma formation in the PDX model than did colorectal cancer (1/43, 2.3%). Statistical analysis revealed that cancer type and inflammation in the parent tumor are significantly associated with lymphomagenesis. Further validation discovered lymphomagenesis by inoculating only gastritis mucosa. Therefore, our findings suggest that it is necessary to take precautions when directly xenografting cancer tissues with remarkable baseline inflammation, such as gastric cancer into immunodeficient NOD/SCID strains. Further, the established xenograft models should be validated by both leukocyte markers and human gene signatures.

语种: 英语
所属项目编号: 2012AA02A203-B01 ; 2012AA02A504-B01 ; 2012AA020101 ; Z121100007512010 ; D131100005313010
项目资助者: National Key Technology RD Program ; Beijing Municipal Science &amp ; Technology Commission
WOS记录号: WOS:000351938100001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53947
Appears in Collections:北京大学临床肿瘤学院_肿瘤外科_期刊论文

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作者单位: 1.Peking Univ, Canc Hosp & Inst, Dept Surg, Key Lab Carcinogenesis & Translat Res,Minist Edu, Beijing 100871, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Dept Pathol, Beijing 100871, Peoples R China
3.BeiGene Beijing Co Ltd, Life Sci Pk, Beijing, Peoples R China

Recommended Citation:
Zhang, Lianhai,Liu, Yiqiang,Wang, Xiaohong,et al. The extent of inflammatory infiltration in primary cancer tissues is associated with lymphomagenesis in immunodeficient mice[J]. SCIENTIFIC REPORTS,2015,5.
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