北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心  > 期刊论文
学科主题: 临床医学
题名:
Effect of Local Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor on Subcutaneously Allotransplanted Ovarian Tissue in Ovariectomized Mice
作者: Gao, Jiangman1,2; Huang, Ying1,2; Li, Min1; Zhao, Hongcui1,3; Zhao, Yue1,2; Li, Rong1,2,3; Yan, Jie1,3; Yu, Yang1,2; Qiao, Jie1,2,3
刊名: PLOS ONE
发表日期: 2015-07-24
DOI: 10.1371/journal.pone.0134035
卷: 10, 期:7
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: FACTOR-INDUCED ANGIOGENESIS ; IN-VITRO ; FOLLICULAR DEVELOPMENT ; FACTOR EXPRESSION ; VEGF ; TRANSPLANTATION ; APOPTOSIS ; PROMOTES ; SURVIVAL ; CELLS
英文摘要:

Objective

One of the major obstacles to ovarian tissue preservation is delayed angiogenesis that leads follicles lost after transplantation. The aim of the present study was to investigate the effects of bFGF and VEGF on heterotopic transplanted ovarian tissue using a mouse model.

Methods

Female mice underwent bilateral ovariectomy. Ovarian tissues encapsulated by fibrin hydrogels were transplanted subcutaneously into recipient mice, in which ovarian hormonal cyclicity was absent. The fibrinogen solution was mixed with bFGF, VEGF, or a mixture of bFGF and VEGF. The grafts were recovered 21 days after transplantation. Follicle morphology and follicle numbers were observed by H&E staining. Blood vessels were observed in transplanted intra-ovarian tissue by CD31 antibody IHC staining. Daily vaginal cytology was performed to determine estrous cycle and functional restoration of transplanted ovarian tissue. Blood was collected weekly and serum FSH levels were measured with a radioimmunoassay kit. Apoptosis analysis was performed by anti-AC-3 staining and survivin mRNA expression.

Results

The number of primordial follicles and secondary follicles in the bFGF+VEGF group was significantly higher than in the control group. The vascular density in the bFGF+VEGF groups were significantly higher than in the bFGF and the VEGF groups; there was no significant difference between the bFGF and VEGF groups. Estrous cycle was earlier in the bFGF+VEGF group compared with the control group; all mice in this group restored ovarian function. Serum FSH levels in the bFGF+VEGF group were significantly lower than in the control group by day 14 post-transplantation. The AC-3-positive in control group was significantly higher compared with bFGF group and VEGF group, and in bFGF+VEGF group was significantly lower than bFGF group and VEGF group. Survivin mRNA expression in bFGF+VEGF group was significantly higher than control group.

Conclusion

The combination of bFGF and VEGF has beneficial effects on follicle survival, angiogenesis, and resumption of estrous cycles.

语种: 英语
所属项目编号: 2011CB944504 ; 2014CB943203 ; 31371521 ; 31230047
项目资助者: Ministry of Science and Technology of China Grants (973 program) ; National Natural Science Funds for general program
WOS记录号: WOS:000358622000194
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53962
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Dept Obstet & Gynecol, Ctr Reprod Med, Hosp 3, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China

Recommended Citation:
Gao, Jiangman,Huang, Ying,Li, Min,et al. Effect of Local Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor on Subcutaneously Allotransplanted Ovarian Tissue in Ovariectomized Mice[J]. PLOS ONE,2015,10(7).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gao, Jiangman]'s Articles
[Huang, Ying]'s Articles
[Li, Min]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gao, Jiangman]‘s Articles
[Huang, Ying]‘s Articles
[Li, Min]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace