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学科主题临床医学
A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta-Glucans
Dong, Bilin1; Li, Dongsheng1; Li, Ruoyu2; Chen, Sharon C. -A.3; Liu, Weihuang4; Liu, Wei5; Chen, Liuqing1; Chen, Yao1; Zhang, Xu1; Tong, Zhongsheng1; Xia, Yun1; Xia, Ping1; Wang, Yan5; Duan, Yiqun1
刊名PLOS ONE
2014-12-09
DOI10.1371/journal.pone.0114113
9期:12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]HOST-DEFENSE ; CHROMOBLASTOMYCOSIS ; RECEPTOR ; INFECTION ; IMMUNITY ; FORMS ; RECOGNITION ; CYTOKINES ; AGENT ; FUNGI
英文摘要

Fonsecaea pedrosoi (F. pedrosoi), a major agent of chromoblastomycosis, has been shown to be recognized primarily by C-type lectin receptors (CLRs) in a murine model of chromoblastomycosis. Specifically, the beta-glucan receptor, Dectin-1, mediates Th17 development and consequent recruitment of neutrophils, and is evidenced to have the capacity to bind to saprophytic hyphae of F. pedrosoi in vitro. However, when embedded in tissue, most etiological agents of chromoblastomycosis including F. pedrosoi will transform into the sclerotic cells, which are linked to the greatest survival of melanized fungi in tissue. In this study, using immunocompetent and athymic (nu/nu) murine models infected subcutaneously or intraperitoneally with F. pedrosoi, we demonstrated that T lymphocytes play an active role in the resolution of localized footpad infection, and there existed a significantly decreased expression of Th17-defining transcription factor Ror gamma t and inefficient recruitment of neutrophils in chronically infected spleen where the inoculated mycelium of F. pedrosoi transformed into the sclerotic cells. We also found that Dectin-1-expressing histocytes and neutrophils participated in the enclosure of transformed sclerotic cells in the infectious foci. Furthermore, we induced the formation of sclerotic cells in vitro, and evidenced a significantly decreased binding capacity of human or murine-derived Dectin-1 to the induced sclerotic cells in comparison with the saprophytic mycelial forms. Our analysis of beta-glucans-masking components revealed that it is a chitin-like component, but not the mannose moiety on the sclerotic cells, that interferes with the binding of beta-glucans by human or murine Dectin-1. Notably, we demonstrated that although Dectin-1 contributed to the development of IL-17A-producing CD3+CD4+ murine splenocytes upon in vitro-stimulation by saprophytic F. pedrosoi, the masking effect of chitin components partly inhibited Dectin-1-mediated Th17 development upon in vitro-stimulation by induced sclerotic cells. Therefore, these findings extend our understanding of the chronicity of chromoblastomycosis.

语种英语
所属项目编号81301376
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000347515300017
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53963
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
2.1 Hosp Wuhan, Ctr Infect Skin Dis, Dept Dermatol, Wuhan, Peoples R China
3.Peking Univ, Res Ctr Med Mycol, Beijing 100871, Peoples R China
4.Wuhan Univ, Med Res Ctr, Wuhan 430072, Peoples R China
5.Univ Sydney, Westmead Hosp, ICPMR Pathol W, Ctr Infect Dis & Microbiol,Lab Serv, Westmead, NSW 2145, Australia
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Dong, Bilin,Li, Dongsheng,Li, Ruoyu,et al. A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta-Glucans[J]. PLOS ONE,2014,9(12).
APA Dong, Bilin.,Li, Dongsheng.,Li, Ruoyu.,Chen, Sharon C. -A..,Liu, Weihuang.,...&Duan, Yiqun.(2014).A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta-Glucans.PLOS ONE,9(12).
MLA Dong, Bilin,et al."A Chitin-Like Component on Sclerotic Cells of Fonsecaea pedrosoi Inhibits Dectin-1-Mediated Murine Th17 Development by Masking beta-Glucans".PLOS ONE 9.12(2014).
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