IR@PKUHSC  > 北京大学药学院
学科主题药学
Europium Complexes as Novel Indicators of Paracellular Diffusion
Xu, Zhihan; Zhang, Chengyue; Zhang, Yue; Yang, Xiaoda1
关键词Europium Complexes Paracellular Permeation Tight Junction Vanadium Toxicity
刊名CHEMISTRY & BIODIVERSITY
2012-09-01
DOI10.1002/cbdv.201100439
9期:9页:1916-1922
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TIGHT JUNCTIONS ; PERMEABILITY ; CACO-2 ; TRANSPORT ; ASSAYS ; CYTOTOXICITY ; VARIABILITY ; DELIVERY ; CHITOSAN ; DRUGS
英文摘要

Measurement of paracellular permeation is an important assay for tight-junction investigations of drug toxicity, especially for metal-based drugs, and routine validation of the integrity of cell monolayers for models of drug absorption. Great efforts have been made in discovery and validation of novel paracellular diffusion indicators. In the present work, we prepared three Eu complexes, i.e., [Eu(dtpa)] (dtpa=diethylenetriaminepentaacetic acid), [Eu(dtpa)(BSA)], and [Eu(dtpa)(PLL)] (PLL=poly(L-lysine)), and tested their permeation properties on Madin?Darby canine kidney (MDCK) cells. The experimental results showed that all three probes were nontoxic to MDCK cells, permeated across MDCK monolayer exclusively via the paracellular pathways, and responded well to the changes on tight junction with high correlation of Papp values to the decrease of trans-epithelial electric resistance (TEER). In addition, time-resolved fluorescence assays were conducted in a high-sensitivity and background-free mode. All these results confirmed the Eu complexes as novel and practical paracellular indicators.

语种英语
WOS记录号WOS:000308715800024
项目编号20971008
资助机构NSFC ; Higher Education of China
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53965
专题北京大学药学院
北京大学药学院_化学生物学系
作者单位1.Peking Univ, State Key Labs Nat & Biomimet Drugs, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xu, Zhihan,Zhang, Chengyue,Zhang, Yue,et al. Europium Complexes as Novel Indicators of Paracellular Diffusion[J]. CHEMISTRY & BIODIVERSITY,2012,9(9):1916-1922.
APA Xu, Zhihan,Zhang, Chengyue,Zhang, Yue,&Yang, Xiaoda.(2012).Europium Complexes as Novel Indicators of Paracellular Diffusion.CHEMISTRY & BIODIVERSITY,9(9),1916-1922.
MLA Xu, Zhihan,et al."Europium Complexes as Novel Indicators of Paracellular Diffusion".CHEMISTRY & BIODIVERSITY 9.9(2012):1916-1922.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xu, Zhihan]的文章
[Zhang, Chengyue]的文章
[Zhang, Yue]的文章
百度学术
百度学术中相似的文章
[Xu, Zhihan]的文章
[Zhang, Chengyue]的文章
[Zhang, Yue]的文章
必应学术
必应学术中相似的文章
[Xu, Zhihan]的文章
[Zhang, Chengyue]的文章
[Zhang, Yue]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。