IR@PKUHSC  > 北京大学药学院
学科主题药学
Europium Complexes as Novel Indicators of Paracellular Diffusion
Xu, Zhihan; Zhang, Chengyue; Zhang, Yue; Yang, Xiaoda1
关键词Europium Complexes Paracellular Permeation Tight Junction Vanadium Toxicity
刊名CHEMISTRY & BIODIVERSITY
2012-09-01
DOI10.1002/cbdv.201100439
9期:9页:1916-1922
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
资助者NSFC ; Higher Education of China ; NSFC ; Higher Education of China
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TIGHT JUNCTIONS ; PERMEABILITY ; CACO-2 ; TRANSPORT ; ASSAYS ; CYTOTOXICITY ; VARIABILITY ; DELIVERY ; CHITOSAN ; DRUGS
英文摘要

Measurement of paracellular permeation is an important assay for tight-junction investigations of drug toxicity, especially for metal-based drugs, and routine validation of the integrity of cell monolayers for models of drug absorption. Great efforts have been made in discovery and validation of novel paracellular diffusion indicators. In the present work, we prepared three Eu complexes, i.e., [Eu(dtpa)] (dtpa=diethylenetriaminepentaacetic acid), [Eu(dtpa)(BSA)], and [Eu(dtpa)(PLL)] (PLL=poly(L-lysine)), and tested their permeation properties on Madin?Darby canine kidney (MDCK) cells. The experimental results showed that all three probes were nontoxic to MDCK cells, permeated across MDCK monolayer exclusively via the paracellular pathways, and responded well to the changes on tight junction with high correlation of Papp values to the decrease of trans-epithelial electric resistance (TEER). In addition, time-resolved fluorescence assays were conducted in a high-sensitivity and background-free mode. All these results confirmed the Eu complexes as novel and practical paracellular indicators.

语种英语
所属项目编号20971008
资助者NSFC ; Higher Education of China ; NSFC ; Higher Education of China
WOS记录号WOS:000308715800024
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53965
Collection北京大学药学院
作者单位1.Peking Univ, State Key Labs Nat & Biomimet Drugs, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Xu, Zhihan,Zhang, Chengyue,Zhang, Yue,et al. Europium Complexes as Novel Indicators of Paracellular Diffusion[J]. CHEMISTRY & BIODIVERSITY,2012,9(9):1916-1922.
APA Xu, Zhihan,Zhang, Chengyue,Zhang, Yue,&Yang, Xiaoda.(2012).Europium Complexes as Novel Indicators of Paracellular Diffusion.CHEMISTRY & BIODIVERSITY,9(9),1916-1922.
MLA Xu, Zhihan,et al."Europium Complexes as Novel Indicators of Paracellular Diffusion".CHEMISTRY & BIODIVERSITY 9.9(2012):1916-1922.
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