学科主题基础医学
Short interfering RNA against the PDCD5 attenuates cell apoptosis and caspase-3 activity induced by Bax overexpression
Chen, LN; Wang, Y; Ma, DL; Chen, YY
关键词apoptosis caspase-3 mitochondria programmed cell death 5 (PDCD5) short interfering RNA (siRNA) TF-1 cell apoptosis related gene-19 (TFAR-19)
刊名APOPTOSIS
2006
DOI10.1007/s104195-005-3134-y
11期:1页:101-111
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]GENE-EXPRESSION PROFILES ; MAMMALIAN-CELLS ; IN-VIVO ; DEATH ; FAMILY ; TFAR19 ; PHOSPHATIDYLSERINE ; TRANSCRIPTOME ; ACTIVATION ; CARCINOMA
英文摘要

The programmed cell death 5 (PDCD5) protein plays an important apoptosis-accelerating role in cells undergoing apoptosis. Decreased expression of PDCD5 has been detected in various human carcinomas. Here we describe that one potent short interfering RNA (siRNA) against the PDCD5 (siPDCD5) specifically inhibits the expression of PDCD5 at both the mRNA and protein level. Cells with decreased PDCD5 expression displayed reduced sensitivity to an apoptotic stimulus induced by Bax overexpression in HeLa, HEK293 and 293T cell lines. Furthermore, we also show that siPDCD5 inhibited both caspase-3 activity and procaspase-3 cleavage. Suppressed expression of PDCD5 attenuates the release of cytochrome c from mitochondria to cytosol induced by Bax overexpression. This phenomenon is accompanied by the reduced translocation of Bax from the cytosol to mitochondria. MTT assay shows that targeted suppression of PDCD5 expression markedly promoted cell proliferation in Hela and HEK293 cell lines. Our data suggests that PDCD5 may exert its effects through pathway of mitochondria by modulating Bax translocation, cytochrome c release and caspase 3 activation directly or indirectly, and that decreased PDCD5 expression may be one of the mechanisms by which tumor cells achieve resistance to apoptotic stimulus induced by anticancer drugs.

语种英语
WOS记录号WOS:000234858200010
引用统计
被引频次:49[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53971
专题北京大学基础医学院_北京大学人类疾病基因研究中心
作者单位1.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
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Chen, LN,Wang, Y,Ma, DL,et al. Short interfering RNA against the PDCD5 attenuates cell apoptosis and caspase-3 activity induced by Bax overexpression[J]. APOPTOSIS,2006,11(1):101-111.
APA Chen, LN,Wang, Y,Ma, DL,&Chen, YY.(2006).Short interfering RNA against the PDCD5 attenuates cell apoptosis and caspase-3 activity induced by Bax overexpression.APOPTOSIS,11(1),101-111.
MLA Chen, LN,et al."Short interfering RNA against the PDCD5 attenuates cell apoptosis and caspase-3 activity induced by Bax overexpression".APOPTOSIS 11.1(2006):101-111.
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