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学科主题: 基础医学
题名:
Suberoylanilide hydroxamic acid (SAHA) inhibits EGF-induced cell transformation via reduction of cyclin D1 mRNA stability
作者: Zhang, Jingjie2; Ouyang, Weiming2; Li, Jingxia2; Zhang, Dongyun2; Yu, Yonghui2; Wang, York2; Li, Xuejun1; Huang, Chuanshu2
关键词: SAHA ; cyclin D1 mRNA stability ; Cell transformation
刊名: TOXICOLOGY AND APPLIED PHARMACOLOGY
发表日期: 2012-09-01
DOI: 10.1016/j.taap.2012.06.012
卷: 263, 期:2, 页:218-224
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: HISTONE DEACETYLASE INHIBITORS ; MOUSE SKIN CARCINOGENESIS ; HUMAN BREAST-CANCER ; KAPPA-B PATHWAY ; JB6 CL41 CELLS ; POSTTRANSCRIPTIONAL REGULATION ; HUMAN KERATINOCYTES ; FACTOR-ALPHA ; EXPRESSION ; ARSENITE
英文摘要:

Suberoylanilide hydroxamic acid (SAHA) inhibiting cancer cell growth has been associated with its downregulation of cyclin D1 protein expression at transcription level or translation level. Here, we have demonstrated that SAHA inhibited EGF-induced Cl41 cell transformation via the decrease of cyclin D1 mRNA stability and induction of G0/G1 growth arrest. We found that SAHA treatment resulted in the dramatic inhibition of EGF-induced cell transformation, cyclin D1 protein expression and induction of G0/G1 growth arrest. Further studies showed that SAHA downregulation of cyclin D1 was only observed with endogenous cyclin D1, but not with reconstitutionally expressed cyclin D1 in the same cells, excluding the possibility of SAHA regulating cyclin D1 at level of protein degradation. Moreover, SAHA inhibited EGF-induced cyclin d1 mRNA level, whereas it did not show any inhibitory effect on cyclin D1 promoter-driven luciferase reporter activity under the same experimental conditions, suggesting that SAHA may decrease cyclin D1 mRNA stability. This notion was supported by the results that treatment of cells with SAHA decreased the half-life of cyclin D1 mRNA from 6.95 h to 2.57 h. Consistent with downregulation of cyclin D1 mRNA stability, SAHA treatment also attenuated HuR expression, which has been well-characterized as a positive regulator of cyclin D1 mRNA stability. Thus, our study identifies a novel mechanism responsible for SAHA inhibiting cell transformation via decreasing cyclin D1 mRNA stability and induction of G0/G1 growth arrest in Cl41 cells. (c) 2012 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: CA112557 ; ES000260 ; ES010344 ; NSFC30928023 ; 30971516
项目资助者: NIH/NCI ; NIH/NIEHS ; NSFC
WOS记录号: WOS:000308058500010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53976
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.NYU, Nelson Inst Environm Med, Sch Med, Tuxedo Pk, NY 10987 USA
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Dept Pharmacol, Sch Basic Med Sci,Inst Syst Biomed, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Jingjie,Ouyang, Weiming,Li, Jingxia,et al. Suberoylanilide hydroxamic acid (SAHA) inhibits EGF-induced cell transformation via reduction of cyclin D1 mRNA stability[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2012,263(2):218-224.
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