IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题临床医学
DEVELOPMENT OF A MULTIPLEX PCR METHOD FOR DETECTION OF THE GENES ENCODING 16S rRNA, COAGULASE, METHICILLIN RESISTANCE AND ENTEROTOXINS IN STAPHYLOCOCCUS AUREUS
Fan, Yiling1,2; Pan, Feng1,2; Paoli, George C.3; Xiao, Yonghong4; Sheng, Haihui5; Shi, Xianming1,2
刊名JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY
2008-12-01
DOI10.1111/j.1745-4581.2008.00144.x
16期:4页:394-411
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Food Science & Technology
资助者Ministry of Science and Technology of China ; Science and Technology Commission of Shanghai municipality ; Ministry of Science and Technology of China ; Science and Technology Commission of Shanghai municipality
研究领域[WOS]Biotechnology & Applied Microbiology ; Food Science & Technology
关键词[WOS]POLYMERASE-CHAIN-REACTION ; MUPIROCIN RESISTANCE ; RAPID DETECTION ; TOXIN GENES ; MECA GENE ; ASSAY ; IDENTIFICATION ; INFECTIONS ; STRAINS ; VIRULENCE
英文摘要

A multiplex polymerase chain reaction (PCR) method was developed for simultaneous detection of the genes encoding methicillin resistance (mecA), SEs A, B and C (sea, seb and sec), coagulase (coa), and 16S rRNA. The primers for amplification of the 16S rRNA gene were specific for Staphylococcus spp., and the primers for coa were specific for Staphylococcus aureus. Based on the results, the multiplex PCR was accomplished at an optimal Mg(2) (+)concentration of 1.0 mM and at an annealing temperature of 56C. This multiplex PCR method was performed with 71 strains of S. aureus and 51 strains of six other bacterial species. Among the S. aureus strains tested, 40.0% (28/71) were found to contain the mecA gene. One of the 28 mecA(+)strains was not resistant to methicillin. The sea, seb and sec genes were present in 47.9% (34/71), 5.6% (4/71) and 8.5% (6/71) of S. aureus strains, respectively. The sensitivity of this multiplex PCR method was approximately 104.5 pg of genomic DNA per reaction, which was equivalent to an estimated 2.4 x 10(3) cfu of S. aureus or 3.64 x 10(4) copies of genome equivalent.

语种英语
所属项目编号2006BAK02A14 ; 071422011 ; 07dz19508
资助者Ministry of Science and Technology of China ; Science and Technology Commission of Shanghai municipality ; Ministry of Science and Technology of China ; Science and Technology Commission of Shanghai municipality
WOS记录号WOS:000261365100011
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53985
专题北京大学第一临床医学院_临床药理研究所
作者单位1.ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA
2.Shanghai Biochip Co Ltd, Shanghai, Peoples R China
3.Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Food Sci, Shanghai 200240, Peoples R China
4.Shanghai Jiao Tong Univ, Sch Agr & Biol, Bor Luh Food Safety Ctr, Shanghai 200240, Peoples R China
5.Peking Univ, Inst Clin Pharmacol, Beijing 100871, Peoples R China
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GB/T 7714
Fan, Yiling,Pan, Feng,Paoli, George C.,et al. DEVELOPMENT OF A MULTIPLEX PCR METHOD FOR DETECTION OF THE GENES ENCODING 16S rRNA, COAGULASE, METHICILLIN RESISTANCE AND ENTEROTOXINS IN STAPHYLOCOCCUS AUREUS[J]. JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY,2008,16(4):394-411.
APA Fan, Yiling,Pan, Feng,Paoli, George C.,Xiao, Yonghong,Sheng, Haihui,&Shi, Xianming.(2008).DEVELOPMENT OF A MULTIPLEX PCR METHOD FOR DETECTION OF THE GENES ENCODING 16S rRNA, COAGULASE, METHICILLIN RESISTANCE AND ENTEROTOXINS IN STAPHYLOCOCCUS AUREUS.JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY,16(4),394-411.
MLA Fan, Yiling,et al."DEVELOPMENT OF A MULTIPLEX PCR METHOD FOR DETECTION OF THE GENES ENCODING 16S rRNA, COAGULASE, METHICILLIN RESISTANCE AND ENTEROTOXINS IN STAPHYLOCOCCUS AUREUS".JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY 16.4(2008):394-411.
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