IR@PKUHSC  > 北京大学基础医学院  > 北京大学系统生物医学研究所
学科主题基础医学
Simultaneous Determination of Six Neurosteroids Hormones in Rat Brain Tissues by High Performance Liquid Chromatography-Tandem Mass Spectrometry
Liu Jia1; Zhang Meng2; Wan You2; Wu Hong-Hai3; Hou Yan-Ning3; Zheng Le-Min1; Yin Yu-Xin1
关键词Neurosteroids Liquid Chromatography-tandem Mass Spectrometry Multiple Reaction Monitoring Rat Brain
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2014-08-01
43期:8页:1118-1124
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Chemistry
关键词[WOS]ELECTROSPRAY-IONIZATION ; STEROIDS ; MS
英文摘要

An analytical method based on high performance liquid chromatography tandem mass spectrometry was developed for the simultaneous determination of 6 neurosteroids in rat brain tissues. The neurosteroids were separated on an Agilent XDB-C-18 column (50 mm x 4.6 mm, 1.8 mu m), using methanol-5 mmol/L ammonium acetate as the mobile phase. A gradient elution program with a cycle time of 10 min was used. The neurosteroids were detected by electrospray ionization mass spectrometry in positive mode with multiple reaction monitoring (MRM) scan mode. For 11-deoxycorticosterone (DOC) and progesterone (PROG), the linear range of calibration curve was from 0.10 ng/mL to 10 ng/mL, and for allopregnanolone (AP), pregnenolone (PREG), dehydroepiandrosterone (DHEA) and allotetrahydrodoc (THDOC), the linear range calibration curve was from 0.50 ng/mL to 10 ng/mL, both with the correlation coefficient more than 0.99. The mean recoveries at spiked levels of 0.4, 4.0 and 8.0 ng/mL were in the range of 91.2%-115.5%, and the RSDs were 0.9%-8.8% (n=6). The limits of detection and quantification were 0.04-0.20 ng/mL and 0.10-0.50 ng/mL, respectively.

语种中文
所属项目编号21305005
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000362824600002
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54003
Collection北京大学基础医学院_北京大学系统生物医学研究所
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Bethune Int Peace Hosp, Shijiazhuang 050082, Peoples R China
3.Peking Univ, Inst Syst Biomed, Hlth Sci Ctr, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Liu Jia,Zhang Meng,Wan You,et al. Simultaneous Determination of Six Neurosteroids Hormones in Rat Brain Tissues by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2014,43(8):1118-1124.
APA Liu Jia.,Zhang Meng.,Wan You.,Wu Hong-Hai.,Hou Yan-Ning.,...&Yin Yu-Xin.(2014).Simultaneous Determination of Six Neurosteroids Hormones in Rat Brain Tissues by High Performance Liquid Chromatography-Tandem Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,43(8),1118-1124.
MLA Liu Jia,et al."Simultaneous Determination of Six Neurosteroids Hormones in Rat Brain Tissues by High Performance Liquid Chromatography-Tandem Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 43.8(2014):1118-1124.
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