IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Steroid-Associated Hip Joint Collapse in Bipedal Emus
Zheng, Li-Zhen1; Liu, Zhong1; Lei, Ming2,6; Peng, Jiang1,4; He, Yi-Xin1; Xie, Xin-Hui1,7; Man, Chi-Wai1; Huang, Le1; Wang, Xin-Luan1,3; Fong, Daniel Tik-Pui1; Xiao, De-Ming2,6; Wang, Da-Ping2; Chen, Yang2; Feng, Jian Q.5; Liu, Ying5; Zhang, Ge1,8; Qin, Ling1,3
刊名PLOS ONE
2013-10-21
DOI10.1371/journal.pone.0076797
8期:10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]FEMORAL-HEAD OSTEONECROSIS ; ACUTE-RESPIRATORY-SYNDROME ; CORTICOSTEROID-INDUCED OSTEONECROSIS ; BONE-MARROW EDEMA ; NONTRAUMATIC OSTEONECROSIS ; PHYTOMOLECULE ICARITIN ; AVASCULAR NECROSIS ; SUBCHONDRAL BONE ; NATURAL-HISTORY ; DEFECT REPAIR
英文摘要

In this study we established a bipedal animal model of steroid-associated hip joint collapse in emus for testing potential treatment protocols to be developed for prevention of steroid-associated joint collapse in preclinical settings. Five adult male emus were treated with a steroid-associated osteonecrosis (SAON) induction protocol using combination of pulsed lipopolysaccharide (LPS) and methylprednisolone (MPS). Additional three emus were used as normal control. Post-induction, emu gait was observed, magnetic resonance imaging (MRI) was performed, and blood was collected for routine examination, including testing blood coagulation and lipid metabolism. Emus were sacrificed at week 24 post-induction, bilateral femora were collected for micro-computed tomography (micro-CT) and histological analysis. Asymmetric limping gait and abnormal MRI signals were found in steroid-treated emus. SAON was found in all emus with a joint collapse incidence of 70%. The percentage of neutrophils (Neut %) and parameters on lipid metabolism significantly increased after induction. Micro-CT revealed structure deterioration of subchondral trabecular bone. Histomorphometry showed larger fat cell fraction and size, thinning of subchondral plate and cartilage layer, smaller osteoblast perimeter percentage and less blood vessels distributed at collapsed region in SAON group as compared with the normal controls. Scanning electron microscope (SEM) showed poor mineral matrix and more osteo-lacunae outline in the collapsed region in SAON group. The combination of pulsed LPS and MPS developed in the current study was safe and effective to induce SAON and deterioration of subchondral bone in bipedal emus with subsequent femoral head collapse, a typical clinical feature observed in patients under pulsed steroid treatment. In conclusion, bipedal emus could be used as an effective preclinical experimental model to evaluate potential treatment protocols to be developed for prevention of ON-induced hip joint collapse in patients.

语种英语
WOS记录号WOS:000326032600011
项目编号GHP/001/08 ; ITS/451/09FP ; SG200810200101A/02/A ; DE018486
资助机构Hong Kong ITC ; Hong Kong ITF ; Shenzhen Science &amp ; Technology Fund ; NIDCR/NIH
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/54008
专题北京大学深圳医院
作者单位1.Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Hong Kong, Hong Kong, Peoples R China
2.Shenzhen Second Peoples Hosp, Dept Orthopaed & Traumatol, Shenzhen, Peoples R China
3.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Translat Med R&D Ctr, Shenzhen, Peoples R China
4.Chinese Peoples Liberat Army, Gen Hosp, Orthoped Res Inst, Beijing, Peoples R China
5.Univ Missouri, Baylor Coll Dent, Kansas City, MO 64110 USA
6.Beijing Univ, Shenzhen Hosp, Dept Orthopead, Shenzhen, Peoples R China
7.Southeast Univ, Zhongda Hosp, Dept Orthopaed, Nanjing, Jiangsu, Peoples R China
8.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Zheng, Li-Zhen,Liu, Zhong,Lei, Ming,et al. Steroid-Associated Hip Joint Collapse in Bipedal Emus[J]. PLOS ONE,2013,8(10).
APA Zheng, Li-Zhen.,Liu, Zhong.,Lei, Ming.,Peng, Jiang.,He, Yi-Xin.,...&Qin, Ling.(2013).Steroid-Associated Hip Joint Collapse in Bipedal Emus.PLOS ONE,8(10).
MLA Zheng, Li-Zhen,et al."Steroid-Associated Hip Joint Collapse in Bipedal Emus".PLOS ONE 8.10(2013).
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