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学科主题: 临床医学
题名:
CMTM3 is reduced in prostate cancer and inhibits migration, invasion and growth of LNCaP cells
作者: Hu, F.1; Yuan, W.2; Wang, X.2; Sheng, Z.1; Yuan, Y.1; Qin, C.1; He, C.1; Xu, T.1
关键词: CMTM3 ; Prostate cancer ; MARVEL ; Tumor suppressor gene ; Gene therapy
刊名: CLINICAL & TRANSLATIONAL ONCOLOGY
发表日期: 2015-08-01
DOI: 10.1007/s12094-015-1288-9
卷: 17, 期:8, 页:632-639
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: SUSCEPTIBILITY LOCI ; EXPRESSION ; METHYLATION ; CARCINOMA ; RECEPTOR ; 16Q22.1 ; GENES
英文摘要:

A novel tumor suppressor gene CKLF-like MARVEL transmembrane domain-containing member 3 (CMTM3) is reduced or undetectable in many kinds of cancers and relates tumor malignant features. We detected its role in prostate cancer for possibility of target therapy as accumulating evidence has shown that CMTM3 is a promising tumor suppressor gene (TSG) for gene therapy.

The expression of CMTM3 detected in prostate tissue microarray, specimens and cell lines were evaluated by immunohistochemistry and semi-quantitative PCR and Western blot, respectively. After being transfected with CMTM3 adenovirus or vector (mock), the proliferation and migration and invasion of LNCaP cells were detected by transwell assay and matrigel assay, respectively. Furthermore, the effects of CMTM3 on tumor growth were performed in nude mice xenograft in vivo.

We found CMTM3 was reduced in PCa tissues and cells compared with BPH tissues, and its expression in PCa tissues was related to the Gleason score. Moreover, after being transfected with adenovirus, ectopic expression of CMTM3 in LNCaP cells led to significant inhibition of cell proliferation and migration and invasion compared with the control (P < 0.05), which may be attributed to decreased Erk1/2 activity as p-Erk1/2 was remarkably reduced when CMTM3 was overexpressed. Finally, restoration of CMTM3 significantly suppressed xenograft tumor growth in vivo (P < 0.01).

语种: 英语
所属项目编号: 31171341
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000358156300007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54033
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Urol, Beijing 100044, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Immunol, Beijing 100191, Peoples R China

Recommended Citation:
Hu, F.,Yuan, W.,Wang, X.,et al. CMTM3 is reduced in prostate cancer and inhibits migration, invasion and growth of LNCaP cells[J]. CLINICAL &amp; TRANSLATIONAL ONCOLOGY,2015,17(8):632-639.
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