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学科主题: 药学
题名:
Separation of 25R/S-ergostane triterpenoids in the medicinal mushroom Antrodia camphorata using analytical supercritical-fluid chromatography
作者: Qiao, Xue1; An, Rong2; Huang, Yun1; Ji, Shuai1; Li, Lang2; Tzeng, Yew-min3; Guo, De-an1; Ye, Min1
关键词: Supercritical-fluid chromatography ; Antrodia camphorata ; 25R/S-Ergostane ; Triterpenoid ; 4-Methylbenzoate cellulose ; 2-Ethylpyridine
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2014-09-05
DOI: 10.1016/j.chroma.2014.06.074
卷: 1358, 页:252-260
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CHIRAL STATIONARY PHASES ; MASS-SPECTROMETRY ; MOBILE-PHASE ; RETENTION ; LIQUID
英文摘要:

Ergostanes are major bioactive constituents of the medicinal mushroom Antrodia camphorata. These tetracyclic triterpenoids usually occur as 25R/S epimeric pairs, which renders their chromatographic separation difficult. In this study, we used analytical supercritical-fluid chromatography (SFC) to separate seven pairs of 25R/S-ergostanes from A. camphorata. The (R)- and (S)-forms for each of the seven pairs could be well resolved (R-s > 1.3) on a Chiralcel OJ-H column (4.6 x 250 mm, 5 mu m, chiral), eluted by 10% MeOH in CO2 at 2 mL/min with a back pressure of 120 bar and a column temperature of 40 degrees C Particularly, this chiral-SFC method could rapidly and efficiently separate low-polarity epimers like antcin A and antcin B, which were very difficult for RP-HPLC. A 3-min preparative-scale method was established to purify (25S)- and (25R)-antcin A for the first time. However, OJ-H column suffered from peak overlapping of different pairs of ergostanes. We found that Princeton 2-ethylpyridine column (2-EP, 4.6 x 250 mm, 3 mu m, achiral) could effectively separate different pairs, although the resolutions for 25-R/S forms of each epimeric pair were not as good as OJ-H column. Meanwhile, all the (25S)-forms showed stronger retentions than the corresponding (25R)-epimers on the 2-EP column. These results demonstrated different selectivity of chiral- and achiral-SFC in separating 25R/S-ergostane epimers. Aside from high separation efficiency, SFC also showed advantage over HPLC in short analysis time and low consumption of organic solvents. Finally, both OJ-H and 2-EP columns were used on analytical SFC to separate 25R/S-ergostanes in an extract of A. camphorata. (C) 2014 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81222054 ; 81303294 ; NCET-11-0019
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education
WOS记录号: WOS:000340979200030
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/54069
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Agilent Technol, Beijing 100102, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Chaoyang Univ Technol, Inst Biochem Sci & Technol, Taichung 41349, Taiwan

Recommended Citation:
Qiao, Xue,An, Rong,Huang, Yun,et al. Separation of 25R/S-ergostane triterpenoids in the medicinal mushroom Antrodia camphorata using analytical supercritical-fluid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1358:252-260.
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